CORE--GENETIC MARKERS

核心——遗传标记

• 批准号: 6450040
• 负责人: RICHARD C DAVIS
• 金额: $ 23.48万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6450040
• 关键词: biomedical facility; computer assisted sequence analysis; familial hyperlipoproteinemia; genetic mapping; genetic markers; genotype; high throughput technology; human genetic material tag; laboratory mouse; nucleic acid sequence; polymerase chain reaction; quantitative trait loci

State-of-the-art genotyping, with its reliance on high throughput PCR and partial automation, is most efficiently carried our in a core laboratory that contains appropriate specialized equipment and staffed by people experienced with the relevant protocols and problems and that is organized for this work. Concentration of genotyping in Core B will also decrease supply costs for the overall Program Project by encouraging bulk purchases of reagents and supplies for use in quantitative trait locus (QTL)1 mapping projects for complex polygenic traits, for construction of primary genetic maps and for sequencing. As gene mapping focuses on specific candidate regions, the work to identify genes underlying atherosclerosis will increasing depend on DNA sequencing to characterize clones, to identify sequence homologies between human and rodent models, and to locate sequence variants leading to differences in gene function. The role for this core is to support all the projects in their requirements for gene mapping and sequencing. Support will be provided by several means with emphasis placed on: 1) High volume scoring of PCR polymorphisms in humans and mice; and 2) the processing and analysis of dideoxy=terminated sequencing reactions produced by the individual projects. In addition, the core will aid the projects in the preparation of genotype and sequence data in formats most useful for further analysis. Human mapping data will be formatted for forwarding to the Biostatistics Core (Core D). For mouse genotype data, the core will assist the individual projects in linkage analysis by Map Manager.

最先进的基因分型依赖于高吞吐量PCR和部分自动化，最有效地将我们带到了一个核心实验室中，该实验室包含适当的专业设备，并由经验丰富的相关协议和问题的人组成，并且该协议和问题是为了组织而组织的，并且是为此而组织的。这项工作。核心B中基因分型的浓度还将通过鼓励大量购买试剂和供应来降低整体程序项目的供应成本，以用于定量性状基因座（QTL）1映射项目，用于构建主要的遗传图和用于测序的基本遗传图和测序。由于基因映射着重于特定候选区域，因此鉴定动脉粥样硬化基因的工作将增加取决于DNA测序以表征克隆，以鉴定人与啮齿动物模型之间的序列同源性，并定位序列变体，从而导致基因功能的差异。该核心的作用是支持所有项目对基因映射和测序的要求。将通过几种重点放在：1）人类和小鼠中PCR多态性的高体积评分； 2）单个项目产生的dideoxy =终止测序反应的处理和分析。此外，核心将帮助项目制备基因型和序列数据的格式，最有用。人体映射数据将格式化以转发到生物统计学核心（核心D）。对于鼠标基因型数据，核心将帮助单个项目通过MAP MANARAR进行链接分析。