Relationship of Blood Esterases, Pesticide Exposure and Cardiovascular Disease

血酯酶、农药暴露与心血管疾病的关系

基本信息

批准号：
7906342
负责人：
Janice Elaine Chambers
金额：
$ 24.76万
依托单位：
MISSISSIPPI STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-04 至 2010-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7906342
关键词：
African American Agriculture Agrochemicals Alabama Arterial Fatty Streak Arylesterase Beds Bile fluid Biochemical Biological Assay Biological Markers Biological Monitoring Blood Blood Vessels Blood specimen Carboxylic Ester Hydrolases Cardiology Cardiovascular Diseases Cardiovascular system Characteristics Cholesterol Cholesterol Esters Clinic Clinical Clinical Research Clinical Trials Communication Coronary Arteriosclerosis Country County Data Data Set Deep South Detection Development Diagnosis Disease Doctor of Philosophy Economics Educational Background Environmental Health Environmental Risk Factor Enzymes Epidemiologic Studies Epidemiologist Epidemiology Etiology Evaluation Excretory function Exposure to Faculty Farming environment Genetic Polymorphism Grant Harvest Health High Density Lipoproteins Hospitals Human Hydrolysis Individual Insecticides Interdisciplinary Study Intervention Isoenzymes Knowledge Laboratories Laboratory Animals Life Liver Medical center Methods Mississippi Modeling Monitor Organophosphates Outcome Patients Pesticides Phenotype Physicians Pilot Projects Population Positioning Attribute Poverty Predisposition Prevalence Principal Investigator Questionnaires Records Recruitment Activity Research Research Personnel Research Project Grants Risk Factors Role Rural Rural Hospitals Sampling Scientist Socioeconomic Status Stroke Study Subject Transport Process United States Universities Urine Visit aryldialkylphosphatase base carboxylesterase design detoxication diagnostic catheterization esterase experience health disparity indexing interest member mortality novel organophosphorus insecticide oxidized low density lipoprotein particle pesticide exposure programs racial difference reverse cholesterol transport tool urinary

项目摘要

DESCRIPTION (provided by applicant): This application for an R21 grant is designed to solidify an interdisciplinary team of basic and clinical researchers in the Center for Environmental Health Sciences at Mississippi State University for research into the environmental factors contributing to the higher mortality of cardiovascular disease (CVD) in the Deep South and among African Americans, and to position this team for participation in larger-scale on- going multi-institutional epidemiological studies. This health disparity in CVD is logically related to risk factors more prevalent in the South (which has a higher proportion of African Americans than other regions in the country.) The South is rural and highly agricultural, so the Southern populations would be expected to be routinely exposed to higher levels of pesticides than populations in many other regions. We hypothesize the following: The effects of pesticide exposure contribute to the development of CVD and are more pronounced in the African American population. Two enzymes that we have studied for many years because of their involvement in the detoxication of many pesticides are paraoxonase (PON1) and carboxylesterase (CaE). The former is known to be associated with HDL particles and the latter has recently been identified as a cholesteryl ester hydrolase that is involved in reverse cholesterol transport; therefore, both of these enzymes are involved in vascular health, and are potential biomarkers of susceptibility to CVD. We have experience biomonitoring urinary pesticide metabolites as an index of current pesticide exposure. We have developed a novel geospatial analysis of pesticide harvested crop records that predicts pesticide exposure to individuals for the previous 35 years of their lives. We have experience in cardiology and access to patients in a cardiology practice. We propose a pilot study to investigate the above listed parameters (i.e., PON1 and CaE in the blood and pesticide metabolites in the urine) of subjects recruited from the cardiology practice, and to calculate strength of associations among these factors, the degree of cardiovascular disease and the pesticide exposure estimated in the geospatial analysis model. This pilot study will be conducted following a year of planning, solidifying the interdisciplinary team, optimizing assays, and developing interactions with the project's consultants. The pilot study will position us to participate in a far more extensive study through interactions with our consultants at the University of Alabama at Birmingham. In addition, we anticipate that we will develop more simplified assay methods for PON1 and CaE that will be more amenable for use as a clinical biomarker.

描述（由申请人提供）：R21赠款的申请旨在巩固密西西比州立大学环境健康科学中心的基本和临床研究人员的跨学科团队，用于研究环境因素的研究，从而有助于较高的南方人以及在非洲人中的心血管疾病（CVD）的较高死亡率，并在非洲人和较大的企业中，以替代了较大的参与者，以实施更大的参与者，以实现更多的参与者，以实现更多的参与者，以实现较大的参与者，以实施较大的参与度，以实现较大的参与。研究。 CVD中的这种健康差异在逻辑上与危险因素有关（非裔美国人比例比该国其他地区的比例更高。）南方是农村和高度农业，因此，预计南部人口将常规地暴露于许多其他地区的野生情况。我们假设以下内容：农药暴露的影响有助于CVD的发展，在非裔美国人人口中更为明显。我们多年来研究的两种酶，由于它们参与许多农药的解毒为二氧氧酶（PON1）和羧酸酯酶（CAE）。已知前者与HDL颗粒相关，后者最近被确定为参与反向胆固醇转运的胆固醇酯水解酶。因此，这两种酶都参与血管健康，并且是对CVD敏感性的潜在生物标志物。我们有生物监测的尿中农药代谢物作为当前农药暴露的指数。我们已经对农药收获的农作物记录进行了新的地理空间分析，该分析可预测其前35年的农药暴露于个人。在心脏病学实践中，我们有心脏病学和患者的经验。我们提出了一项试点研究，以调查上述参数（即尿液中的血液和农药代谢物中的PON1和CAE）对从心脏病学实践募集的受试者中的血液和农药代谢产物）进行了研究，并计算了这些因素之间的关联性强度，心血管疾病的程度，估计的载有脂肪的程度。这项试点研究将在一年的计划之后进行，巩固跨学科团队，优化测定法，并与项目顾问进行互动。试点研究将使我们通过与阿拉巴马大学伯明翰大学的顾问进行互动来参加更广泛的研究。此外，我们预计将针对PON1和CAE开发更多简化的测定方法，这些方法将更适合用作临床生物标志物。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Racial Differences in Paraoxonase-1 (PON1): A Factor in the Health of Southerners?

DOI：
10.1289/ehp.0900569
发表时间：
2009-08-01
期刊：
ENVIRONMENTAL HEALTH PERSPECTIVES
影响因子：
10.4
作者：
Davis, Kimberly A.;Crow, J. Allen;Chambers, Janice E.
通讯作者：
Chambers, Janice E.

Relationship of human paraoxonase-1 serum activity and genotype with atherosclerosis in individuals from the Deep South.

DOI：
10.1097/fpc.0b013e32834cebc6
发表时间：
2011-12
期刊：
Pharmacogenetics and genomics
影响因子：
2.6
作者：
Coombes RH;Crow JA;Dail M;Chambers HW;Wills RW;Barry D. Bertolet for CARe;Chambers JE
通讯作者：
Chambers JE

The association of serum trans-nonachlor levels with atherosclerosis.