High Resolution LC-MS/MS System

高分辨率 LC-MS/MS 系统

• 批准号: 7595425
• 负责人: PETER LOBEL
• 金额: $ 50万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7595425
• 关键词: Area; Biological; Biomedical Research; Communicable Diseases; Funding; High Pressure Liquid Chromatography; Institution; Liquid Chromatography; Malignant Neoplasms; Mass Spectrum Analysis; Neurodegenerative Disorders; Post-Translational Protein Processing; Proteins; Proteomics; Request for Applications; Research; Research Personnel; Resolution; Solvents; Source; System; United States National Institutes of Health; Universities; Wood material; comparative; crosslink; instrument; instrumentation; mass spectrometer; medical schools; meetings; public health relevance; tandem mass spectrometry

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase instrumentation to conduct high-resolution liquid chromatography tandem mass spectrometry (LC-MS/MS). The instrument will be located in an existing biological mass spectrometry facility on the Piscataway campus of Robert Wood Johnson Medical School (RWJMS) and Rutgers University. A high resolution LC-MS/MS instrument will greatly expand the capabilities of the facility to meet the increasing research needs of 31 NIH-funded investigators. Funds from NIH along with institutions support will allow the acquisition of a ThermoFisher LTQ Orbitrap XL ETD mass spectrometer, a Proxeon nanospray source, and a Dionex Ultimate 3000 HPLC system. There are no comparable instruments at RWJMS and Rutgers. The instrument will be used for characterization of post-translational modifications on proteins, quantitative analysis/comparative proteomics, crosslinking analysis, solvent accessibility studies, and other application that require high-resolution LC-MS/MS capabilities. This will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer. PUBLIC HEALTH RELEVANCE: This is an application requesting funds to acquire a specialized mass spectrometer that will be used for projects being conducted by 31 NIH-supported investigators. The availability of this instrument will accelerate the pace of basic and applied biomedical research into important areas including neurodegenerative disease, infectious disease, and cancer.

描述（由申请人提供）：这是一项申请，要求资金购买仪器以进行高分辨率液相色谱串联质谱法（LC-MS/MS）。该乐器将位于罗伯特·伍德·约翰逊医学院（RWJMS）和罗格斯大学的Piscataway校园的现有生物质谱设施中。高分辨率LC-MS/MS仪器将大大扩展该设施的能力，以满足31位NIH资助的研究人员的研究需求。 NIH的资金以及机构的支持将允许收购Thermofisher LTQ Orbitrap XL ETD质谱仪，Proxeon Nanospray源和Dionex Ultimate 3000 HPLC系统。 RWJM和Rutgers没有可比的乐器。该仪器将用于表征对蛋白质的翻译后修饰，定量分析/比较蛋白质组学，交联分析，溶剂可访问性研究以及其他需要高分辨率LC-MS/MS功能的应用。这将加速基本和应用生物医学研究的速度，包括神经退行性疾病，传染病和癌症。公共卫生相关性：这是一项申请，要求资金获得专门的质谱仪，该质谱仪将用于由31位NIH支持的调查人员进行的项目。该仪器的可用性将加速基本和应用生物医学研究的速度，包括神经退行性疾病，传染病和癌症。