A Mass Spectrometry System for Quantitative Proteomics

用于定量蛋白质组学的质谱系统

基本信息

批准号：
8640415
负责人：
PETER LOBEL
金额：
$ 56.13万
依托单位：
RBHS-ROBERT WOOD JOHNSON MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2015-06-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): This is an application requesting funds to purchase a Q Exactive mass spectrometer and liquid chromatography system that will be used for quantitative proteomics research. The instrument will be integrated into the Robert Wood Johnson Medical School/Rutgers University Biological Mass Spectrometry Facility. Current instrumentation in the Facility is used at maximal capacity and we require additional capabilities to conduct quantitative proteomics experiments for thirteen NIH-funded investigators. Our user group will apply quantitative mass spectrometry to most efficiently address a wide range of important biomedical research areas including neurological disease, bacterial and viral infection, cancer, anaphylaxis, diabetes, digestive disease, and sleep disorders. Specific experiments include quantification of changes in levels and post-translational modifications of clock proteins throughout the circadian cycle, a systems biology approach to understand the lysosome and its role in human neurodegenerative disease, persistence of epitopes on protein food allergens under conditions that simulate gastric digestion, dynamics of post- translational modification of tumor suppressors during oncogenesis and analysis of pathogen-host interactions at different stages in their life cycle for hepatitis C virus, retroviruses, and chlamydia.. The requested instrument will therefore represent a significant asset to our research community and will accelerate the pace of basic and applied NIH-funded health-research at Rutgers University and the Robert Wood Johnson Medical School of UMDNJ.

描述（由申请人提供）：这是一项申请，要求资金购买Q精确的质谱仪和液相色谱系统，该系统将用于定量蛋白质组学研究。该乐器将集成到罗伯特·伍德·约翰逊医学院/罗格斯大学生物学质谱设施中。该设施中的当前仪器以最大能力使用，我们需要其他功能来为13名NIH资助的研究人员进行定量蛋白质组学实验。我们的用户组将使用定量质谱法来最有效地解决广泛的重要生物医学研究领域，包括神经系统疾病，细菌和病毒感染，癌症，过敏反应，糖尿病，消化系统疾病和睡眠障碍。具体的实验包括量化水平的变化和整个昼夜节律的平时蛋白质的变化和翻译后的修饰，一种系统生物学方法，以了解溶酶体及其在人类神经退行性疾病中的作用及其在蛋白质食物过敏剂对蛋白质过敏剂的持久性在胃挖掘和分析剂量的抑制作用后，蛋白质食物过敏剂的持久性，在蛋白质过敏剂中进行了分析。因此，在其生命周期中，丙型肝炎病毒，逆转录病毒和衣原体。因此，所请求的工具将代表我们的研究社区的重要资产，并将加速Rutgers University和Robert Wood Johnson Medical School of Umdnj的基本和应用NIH资助的健康研究的步伐。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

A multipronged approach unravels unprecedented protein-protein interactions in the human 2-oxoglutarate dehydrogenase multienzyme complex.

多管齐下的方法揭示了人类 2-酮戊二酸脱氢酶多酶复合物中前所未有的蛋白质-蛋白质相互作用。

DOI：
10.1074/jbc.ra118.005432
发表时间：
2018
期刊：
The Journal of biological chemistry
影响因子：
0
作者：
Zhou,Jieyu;Yang,Luying;Ozohanics,Oliver;Zhang,Xu;Wang,Junjie;Ambrus,Attila;Arjunan,Palaniappa;Brukh,Roman;Nemeria,NataliaS;Furey,William;Jordan,Frank
通讯作者：
Jordan,Frank

Elemental sulfur reduction in the deep-sea vent thermophile, Thermovibrio ammonificans.

深海喷口嗜热菌、氨化热弧菌中元素硫的还原。