Quantifying Effector Functions of Anti-HIV IgG1 Antibodies In Vivo.

体内量化抗 HIV IgG1 抗体的效应器功能。

基本信息

批准号：
10866743
负责人：
DAVID D HO
金额：
$ 49.98万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10866743
关键词：
Address Animals Antibodies Antibody Response Antibody Therapy Antigen Presentation Applications Grants Area Binding Blood CD4 Positive T Lymphocytes Cells Complement-Dependent Cytotoxicity Complex Data Data Set Death Rate Disease Equation Fc Receptor HIV HIV Antibodies HIV Infections HIV vaccine Half-Life Human IgG Receptors IgG1 Immunoglobulin G Immunology In Vitro Infection Knowledge Macaca mulatta Mathematics Measures Mediating Monkeys Monoclonal Antibodies Mus Mutation NIH Program Announcements Patients Persons Plasma Population Productivity Property Series Specific qualifier value Vaccine Design Vaccines Viral Viral Load result Viral Physiology Viremia Virion Virus Virus Diseases antibody-dependent cell cytotoxicity antibody-dependent cellular phagocytosis antiretroviral therapy comparative expectation experimental study humanized mouse improved in vivo insight mathematical analysis mouse model neutralizing antibody particle response simian human immunodeficiency virus vaccine discovery vaccine-induced antibodies viral RNA ward

项目摘要

PROJECT SUMMARY/ABSTRACT There is an abundance of evidence demonstrating the importance of Fc-mediated effector function to the overall activity of an antibody in vivo. However, the relative contribution of virus neutralization versus effector functions to the antiviral effect of an antibody remains undefined. We have proposed a series of experiments that will quantify the contributions of Fc-mediated effector functions to the overall activity of an antibody. The quantitative experiments will be performed in the setting of antibody treatment of SHIV infection in rhesus macaques. These fundamental questions in immunology have yet to be answered, and the resultant information promises to provide important insights on how the two major properties (neutralization versus effector functions) of a vaccine- induced IgG response combine forces to ward off the establishment of HIV infection.

项目概要/摘要有大量证据表明 Fc 介导的效应器功能对整体的重要性抗体在体内的活性。然而，病毒中和与效应功能的相对贡献抗体的抗病毒作用仍不确定。我们提出了一系列实验量化 Fc 介导的效应功能对抗体整体活性的贡献。定量实验将在恒河猴 SHIV 感染的抗体治疗中进行。这些免疫学中的基本问题尚未得到解答，由此产生的信息有望提供有关疫苗的两个主要特性（中和作用与效应器功能）如何发挥作用的重要见解诱导的 IgG 反应联合起来阻止 HIV 感染的建立。