Quantifying Effector Functions of Anti-HIV IgG1 Antibodies In Vivo.

体内量化抗 HIV IgG1 抗体的效应器功能。

• 批准号: 10239076
• 负责人: DAVID D HO
• 金额: $ 24.77万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10239076
• 关键词: Address; Animals; Antibodies; Antibody Response; Antibody Therapy; Antigen Presentation; Antiviral Agents; Applications Grants; Area; Binding; Blood; CD4 Positive T Lymphocytes; Cells; Complement-Dependent Cytotoxicity; Complex; Data; Data Set; Death Rate; Disease; Ensure; Equation; Fc Receptor; HIV; HIV Antibodies; HIV Infections; HIV vaccine; Half-Life; Human; IgG Receptors; IgG1; Immunoglobulin G; Immunology; In Vitro; Infection; Knowledge; Macaca mulatta; Mathematics; Measures; Mediating; Monkeys; Mus; NIH Program Announcements; Patients; Persons; Plasma; Property; Series; Specific qualifier value; Vaccine Design; Vaccines; Viral; Viral Antibodies; Viral Load result; Viremia; Virion; Virus; Virus Diseases; antibody-dependent cell cytotoxicity; antibody-dependent cellular phagocytosis; antiretroviral therapy; comparative; expectation; experimental study; humanized mouse; improved; in vivo; insight; mathematical analysis; mouse model; neutralizing antibody; particle; response; simian human immunodeficiency virus; vaccine discovery; vaccine-induced antibodies; viral RNA; ward

PROJECT SUMMARY/ABSTRACT There is an abundance of evidence demonstrating the importance of Fc-mediated effector function to the overall activity of an antibody in vivo. However, the relative contribution of virus neutralization versus effector functions to the antiviral effect of an antibody remains undefined. We have proposed a series of experiments that will quantify the contributions of Fc-mediated effector functions to the overall activity of an antibody. The quantitative experiments will be performed in the setting of antibody treatment of SHIV infection in rhesus macaques. These fundamental questions in immunology have yet to be answered, and the resultant information promises to provide important insights on how the two major properties (neutralization versus effector functions) of a vaccine- induced IgG response combine forces to ward off the establishment of HIV infection.

项目摘要/摘要 有很多证据表明FC介导的效应子功能对整体的重要性 体内抗体的活性。但是，病毒中和与效应子功能的相对贡献 抗体的抗病毒作用仍然不确定。我们提出了一系列实验 量化FC介导的效应子功能对抗体的整体活性的贡献。定量 实验将在恒河猕猴中SHIV感染的抗体治疗中进行。这些 免疫学中的基本问题尚未得到回答，结果信息有望 就疫苗的两个主要特性（中和与效应函数）如何提供重要见解。 诱导的IgG反应结合了力量来阻止艾滋病毒感染的建立。