Specific Pathogen Free 18 Baboon Research Resource (SPF18BRR): Applied Research

无特定病原体 18 狒狒研究资源 (SPF18BRR)：应用研究

• 批准号: 10627138
• 负责人: Joe H. Simmons
• 金额: $ 6.05万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2028-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627138
• 关键词: 15 year old; 3 year old; Adult; Age; Aging; Alzheimer' s disease related dementia; Animal Model; Animals; Applications Grants; Applied Research; Biological Markers; Biomedical Research; Breeding; Cerebrospinal Fluid; Cognition; DNA Methylation; Diabetes Mellitus; Doctor of Philosophy; Epigenetic Process; Female; Foundations; Genetic; Human; Intramural Research Program; Measures; Neurosciences; Papio; Papio anubis; Pharmacologic Substance; Plasma; Population Genetics; Primates; Privatization; Research; Research Contracts; Resources; System; Training; United States National Institutes of Health; Virus; age group; animal breeding; cognitive testing; genome sequencing; germ free condition; human disease; human model; improved; male; model development; nonhuman primate; offspring; pathogen; social; whole genome

PROJECT SUMMARY: APPLIED RESEARCH COMPONENT The SPF18BRR is the only national research resource of adventitious virus free olive baboons (Papio anubis) that are available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the entire world, the baboons in the SPF18BRR have an extensive bioexclusion list of 18 pathogens normally found in other wild and captive nonhuman primate colonies. The applied research proposed in this grant application has been developed to strengthen and improve the resource. The first two specific aims are intended to further develop the resource and to demonstrate the value of the SPF18 baboons as an animal model of human Alzheimer’s disease and related dementias (AD/ADRD). Specific aim one will develop and place hardware for automated cognitive testing in socially living baboons. Specific aim two expands on the AD/ADRD model development by calculating a DNA methylation clock for baboons in the SPF18BRR and by measuring a panel of 30 biomarkers on all animals 3 years of age and older at two different timepoints. Additionally, we will collect cerebrospinal fluid from animals 15 years of age and older and will use it to measure a panel of 18 human neuroscience biomarkers. In specific aim three we will perform whole genome sequencing (WGS) on nine olive baboons that were bred at the Southwest National Primate Research Center that we would like to add to our conventional baboon breeding colony to diversify the population genetics before we close the SPF18 colony to new animal introductions. We will also perform targeted WGS on additional animals to further expand the understanding of the genetics of the SPF18BRR.

项目摘要：应用研究部分 SPF18BRR 是唯一国家级无外来病毒橄榄狒狒 (Papio anubis) 研究资源 可供 NIH 受资助者使用的联邦机构内部研究项目，包括 FDA、NSF 和 NIH；以及生物医学研究的其他赞助商（私人基金会、制药公司和 SPF18BRR 中的狒狒在全世界绝对独一无二。 广泛的生物排除清单，包含通常在其他野生和圈养非人类灵长类动物中发现的 18 种病原体 本拨款申请中提出的应用研究旨在加强和加强殖民地。 前两个具体目标旨在进一步开发资源并 证明 SPF18 狒狒作为人类阿尔茨海默病及相关疾病动物模型的价值 具体目标是开发和放置用于自动认知测试的硬件。 具体目标二是通过计算 DNA 来扩展 AD/ADRD 模型的开发。 SPF18BRR 中狒狒的甲基化时钟，并通过测量所有动物的一组 30 个生物标记物 3 此外，我们将在两个不同的时间点收集脑脊液。 15 岁及以上的动物，并将用它来测量一组 18 种人类神经科学生物标志物。 在具体目标三中，我们将对培育的九只橄榄狒狒进行全基因组测序（WGS） 在西南国家灵长类研究中心，我们想将其添加到我们的传统狒狒中 在我们关闭 SPF18 群体新动物之前，繁殖群体使群体遗传学多样化 我们还将对其他动物进行有针对性的全基因组测序，以进一步扩大了解。 SPF18BRR 的遗传学。