Specific Pathogen Free Baboon Research Resource (SPFBRR) - A&R Administrative Supplement

无特定病原体狒狒研究资源 (SPFBRR) - A

• 批准号: 10402650
• 负责人: Joe H. Simmons
• 金额: $ 13.09万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10402650
• 关键词: Address; Administrative Supplement; Animals; Antigens; Award; Biological Assay; Biomedical Research; Breeding; Complex; DNA; Ensure; Equipment; Foundations; Funding; Goals; Individual; Intramural Research Program; Molecular Diagnostic Testing; Papio; Papio anubis; Parents; Pathogenesis; Pharmacologic Substance; Plasma; Privatization; RNA; Reagent; Research; Research Contracts; Research Personnel; Resources; Running; Saliva; Sampling; Sensitivity and Specificity; Serology; Serology test; Standardization; System; Testing; United States National Institutes of Health; Viral Pathogenesis; Virus; Virus Diseases; Work; digital; germ free condition; high throughput screening; improved; pathogen; synthetic polymer Bioplex

Project Summary The Specific Pathogen Free Baboon Research Resource (SPFBRR) is the only national research resource of SPF18 baboons available to NIH grantees; intramural research programs of federal agencies, including the FDA, NSF and NIH; and other sponsors of biomedical research (private foundations, pharmaceutical companies, and contract research organizations). Absolutely unique in the world, the olive baboons (Papio anubis) in the SPFBRR have an extensive bioexclusion list of 18 pathogens normally found in wild and captive baboon colonies. In fact, baboons from the SPFBRR are free of all known adventitious viruses that commonly infect wild and captive baboons. A major challenge during the first few years following relocation of the colony to the Keeling Center has been developing the extensive repertoire of serology and PCR testing assays and strategies to ensure that the SPF18 animals remain free of excluded pathogens. This A&R Administrative Supplement will allow us to purchase two pieces of equipment: a Bio-Plex 200 System to develop inhouse baboon specific multiplexed serology assays, and a QX200 ddPCR System elucidate the pathogenesis of incipient virus infections in naïve olive baboons. This request directly addresses Specific Aim 2 of the P40 Resource Core to expand the breeding resource and Specific Aim 1 of the Research Core to develop molecular diagnostic assays to support the SPFBRR. The proposed study is within the scope of the parent award and will stimulate additional activities leading to the progress of the overall goals of the parent award. The proposed study does not overlap with already funded work in the parent award.

项目概要 无特定病原体狒狒研究资源 (SPFBRR) 是唯一的国家级 向 NIH 受资助者提供 SPF18 狒狒研究资源； 联邦机构，包括 FDA、NSF 和 NIH 以及其他生物医学赞助者； 研究（私人基金会、制药公司和合同研究组织）。 SPFBRR 中的橄榄狒狒 (Papio anubis) 在世界上绝对独一无二 广泛的生物排除清单，包含通常在野生和圈养狒狒群体中发现的 18 种病原体。 事实上，来自SPFBRR的狒狒没有感染所有已知的外来病毒，这些病毒通常 感染野生和圈养狒狒是接下来几年的主要挑战。 殖民地搬迁到基林中心一直在开发广泛的曲目 血清学和 PCR 检测分析和策略，以确保 SPF18 动物保持自由 本 A&R 行政补充文件将允许我们购买两种病原体。 设备：Bio-Plex 200 系统，用于开发内部狒狒特异性多重检测 血清学检测和 QX200 ddPCR 系统阐明了初期病毒的发病机制 该请求直接针对 P40 的具体目标 2。 资源核心扩大育种资源，研究核心的具体目标 1 开发分子诊断分析来支持 SPFBRR 拟议的研究属于该研究范围。 家长奖励的范围，并将激发额外的活动，从而推动该项目的进展 拟议的研究与已资助的研究不重叠。 从事家长奖工作。