Flow Cytometry Core

流式细胞术核心

• 批准号: 10270976
• 负责人: David S. Bradley
• 金额: $ 14.81万
• 依托单位: UNIVERSITY OF NORTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-13 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10270976
• 关键词: Advisory Committees; Applications Grants; Biomedical Research; Biotechnology; Calibration; Cell Separation; Cells; Centers of Research Excellence; Collection; Communicable Diseases; Computer software; Consultations; Data; Data Analyses; Educational workshop; Ensure; Experimental Designs; Fee-for-Service Plans; Fees; Financial compensation; Floor; Flow Cytometry; Fluorochrome; Funding; Human; Immunology; Institution; Instruction; Laboratories; Licensing; Maintenance; Malignant Neoplasms; Microbiology; North Dakota; Pathology; Periodicity; Phase; Research Personnel; Schedule; Services; Site; Software Design; Sorting - Cell Movement; Stains; Training; Universities; Update; cell preparation; design; experimental study; instrument; instrumentation; medical schools; member; nutrition; operation; pathogen; webinar

Summary The North Dakota Flow Cytometry and Cell Sorting (ND-FCCS) Core was established to provide investigators and their laboratory staff associated with this COBRE, the North Dakota IDeA Networks of Biomedical Research Excellence (INBRE), and the Dakota Cancer Collaborative on Translational Activity (DaCCoTA) CTR, as well as researchers across the state of North Dakota, the following: consultation in the design of fluorescent cell analysis or sorting experiments including fluorochrome selection and parameter calibration; training and oversight on the instrumentation to collect flow cytometry data; flow cytometric cell sorting; consultation on data analysis and usage of analysis software; as well as maintenance, upkeep, and when appropriate, upgrading of the instrumentation and analysis software. During Phase 1, the Core was utilized heavily by all of the researchers holding COBRE projects as well as most of the laboratories associated with the larger infectious disease group, investigators in the ND INBRE at UND, NDSU, and Minot State University, and other non-associated investigators at UND, NDSU, and the USDA Human Nutrition Center. During Phase 1 the Core was able to replace the obsolete BD LSR2 with a BD Symphony with funds from both this COBRE and the ND INBRE, and later to add a third flow cytometer, a Sony MA900 Cell Sorter, with funds from the DaCCoTA CTR, as well as to increase the number of licenses for FlowJo analytical software held by the Core for use predominately by the HPI COBRE investigators. The Core will continue with the services provided moving into Phase 2, as well as initiating full service for those investigators wanting the Core to perform the collection of flow cytometric data on their prepared/stained cells, as well as continued fluorescent cell sorting for the COBRE, INBRE, and CTR investigators, as well as non-associated researchers across North Dakota. To ensure ongoing sustainability, a fee for service or instrument usage fee will be implemented during the Phase 2 period of the COBRE for all users of the Core.

概括 北达科他州流式细胞术和细胞分选 (ND-FCCS) 核心的建立是为了向研究人员提供 以及与此 COBRE（北达科他州 IDeA 生物医学网络）相关的实验室工作人员 卓越研究 (INBRE) 和达科他癌症转化活动合作组织 (DaCCoTA) CTR 以及北达科他州的研究人员提出以下建议：设计咨询 荧光细胞分析或分选实验，包括荧光染料选择和参数校准； 对收集流式细胞术数据的仪器进行培训和监督；流式细胞术细胞分选； 数据分析及分析软件使用咨询；以及维护、保养和何时 适当升级仪器和分析软件。在第一阶段，使用了核心 持有 COBRE 项目的所有研究人员以及大多数相关实验室的大力支持 更大的传染病小组、UND、NDSU 和迈诺特州立大学 ND INBRE 的研究人员， 以及 UND、NDSU 和 USDA 人类营养中心的其他非相关研究人员。阶段期间 1 Core 能够用 BD Symphony 替换过时的 BD LSR2，资金来自 COBRE 和 ND INBRE，后来又添加了第三台流式细胞仪，一台索尼 MA900 细胞分选仪，资金来自 DaCCoTA CTR，以及增加核心持有的 FlowJo 分析软件许可证数量 主要由 HPI COBRE 研究人员使用。核心将继续提供服务 进入第二阶段，并为那些希望核心执行任务的调查人员提供全面服务 收集其制备/染色细胞的流式细胞术数据，以及持续的荧光细胞分选 供 COBRE、INBRE 和 CTR 研究人员以及北达科他州的非相关研究人员使用。 为确保持续的可持续性，活动期间将收取服务费或仪器使用费 适用于核心所有用户的 COBRE 第 2 阶段。