Flow Cytometry

流式细胞术

基本信息

批准号：
9924566
负责人：
David S. Bradley
金额：
$ 16.49万
依托单位：
UNIVERSITY OF NORTH DAKOTA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至 2021-08-04
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9924566
关键词：
Area Analyses Basic Science Birds Cell Separation Cell Survival Cells Centers of Research Excellence Collaborations Communicable Diseases Communities Computer software Consult Consultations Containment of Biohazards Contract Services Core Facility Data Discipline Educational workshop Ensure Environment Equipment Experimental Designs Faculty Faculty Recruitment Floor Flow Cytometry Fostering Funding Future Goals Grant Growth Human Immune response Infectious Diseases Research Institution Investments Laboratories Lead Leadership Maintenance Methods Microbe North Dakota Nutritional Study Postdoctoral Fellow Productivity Research Research Activity Research Institute Research Personnel Resolution Resources Role Scholarship Services Sorting - Cell Movement Speed Students Support Contracts Supporting Cell Technology Therapeutic Time Training Training and Education United States National Institutes of Health Universities Update base experience foot graduate student innovation instrumentation lectures meetings member mid-career faculty pathogen posters professor programs repaired success undergraduate student

项目摘要

Project Summary Summary: The Flow Cytometry and Cell Sorter Core Facility, is equipped with a BD LSR II and a BD FACSAria III flow cytometer, which provide the investigators high speed, high resolution, high purity and viability of cell sorting to fit the application of our COBRE investigators. This core facilitates end users on their research involving the study of infectious disease. The overall goal of Flow Cytometry and Cell Sorter Core Facility is to provide various levels of support on comprehensive flow cytometric analysis and sorting, to ensure the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators. The establishment of this core will also foster collaboration and innovation among investigators and members of the academic research community in North Dakota. This core will also educate our undergraduate students and graduate students with the application and the current advances in flow cytometry and cell sorter technology by workshop, lectures and lab-based courses. According to the primary goal of ensuring the success of the Center of Biomedical Research Excellence in the Host-Pathogen Interactions and of our COBRE junior investigators, establishment of the Flow Cytometry and Cell Sorter Core is therefore essential to meet the current and future needs of UND and neighboring institutions, and will be of great importance for the overall success of this grant. Therefore, The Flow Cytometry and Cell Sorting Core is planned as a permanent research core in the UND SMHS that we will establish, grow, and transit to independent core over a 15-year period of COBRE support. The major roles of this Flow Cytometry and cell sorting Core Facility are: Role 1: To provide our COBRE and other biomedical investigators with various levels of support on comprehensive flow cytometric analysis and sorting including instrumentation, technical support and consultation. Role 2: To educate faculty, staff, especially graduate students about the application and the current advances in flow cytometry and cell sorter technology.

项目概要摘要：流式细胞仪和细胞分选仪核心设施配备了 BD LSR II 和 BD FACSAria III 流式细胞仪，为研究人员提供高速、高分辨率、高纯度和细胞分选的可行性，以适应我们 COBRE 研究人员的应用。该核心有助于最终用户他们的研究涉及传染病的研究。流式细胞术和细胞分选仪的总体目标核心设施是为综合流式细胞术分析和分选提供各种级别的支持，以确保卓越生物医学研究中心在宿主-病原体相互作用方面取得成功我们的 COBRE 初级调查员。该核心的建立也将促进协作和创新北达科他州的调查人员和学术研究界的成员。该核心还将向我们的本科生和研究生介绍应用程序和当前的进展通过研讨会、讲座和实验室课程介绍流式细胞术和细胞分选技术。根据确保宿主病原体生物医学研究卓越中心取得成功的主要目标我们的 COBRE 初级研究人员的互动，流式细胞仪和细胞分选仪的建立因此，核心对于满足联合国开发计划署和邻近机构当前和未来的需求至关重要，并将对于本次赠款的整体成功非常重要。因此，流式细胞术和细胞分选核心计划作为 UND SMHS 的永久研究核心，我们将建立、发展并过渡到该核心独立核心超过 15 年的 COBRE 支持。流式细胞术和细胞的主要作用排序核心设施是：角色 1：为我们的 COBRE 和其他生物医学研究人员提供各种对综合流式细胞术分析和分类的支持水平，包括仪器、技术支持和咨询。作用 2：对教职员工，特别是研究生进行有关申请的教育以及流式细胞术和细胞分选技术的最新进展。