VIRAL LOCALIZATION BY IN SITU HYBRIDIZATION

通过原位杂交进行病毒定位

• 批准号: 7958290
• 负责人: Shawn Patrick O'Neil
• 金额: $ 6.23万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958290
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Anti-Retroviral Agents; Asians; Cells; Computer Retrieval of Information on Scientific Projects Database; Cytomegalovirus; DNA Sequence; Funding; Grant; HIV; HIV Infections; Human; In Situ Hybridization; Infection; Infection prevention; Institution; Macaca; Measures; Modeling; Molecular Probes; Monkeys; New England; Parvovirus; Pathogenesis; Primates; RNA; Research; Research Personnel; Resources; SIV; SRV-1; Saimiri; Simian virus 40; Source; System; Tissues; United States National Institutes of Health; Viral; Virus; base; design; pathogen; simian human immunodeficiency virus; vaccine efficacy; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The infection of Asian macaque monkeys with simian immunodeficiency virus (SIV) and SIV/HIV chimeric viruses (SHIVs) serves as an important animal model for studying the pathogenesis of HIV infection and AIDS. In addition, this model provides a challenge system for evaluating the efficacy of vaccines and antiretroviral agents designed to prevent infection or AIDS in humans. We have designed several molecular probes for localizing viral RNA in tissues of monkeys infected with various isolates and clones of SIV and SHIV. These probes are used to measure the quantity and distribution of infected cells in the tissues of infected monkeys. We also have probes that hybridize with target RNA or DNA sequences of several other viral pathogens, including HIV, SRV-1, CMV, SV40, EBV, Herpes saimiri, and parvovirus.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 感染猿猴免疫缺陷病毒（SIV）和SIV/HIV嵌合病毒（SHIV）的亚洲猕猴是研究HIV感染和艾滋病发病机制的重要动物模型。 此外，该模型还提供了一个挑战系统，用于评估旨在预防人类感染或艾滋病的疫苗和抗逆转录病毒药物的功效。 我们设计了几种分子探针，用于定位感染 SIV 和 SHIV 的各种分离株和克隆的猴子组织中的病毒 RNA。 这些探针用于测量受感染猴子组织中受感染细胞的数量和分布。 我们还有与其他几种病毒病原体的目标 RNA 或 DNA 序列杂交的探针，包括 HIV、SRV-1、CMV、SV40、EBV、塞米里疱疹病毒和细小病毒。