IMMUNOHISTOCHEMICAL IDENTIFICATION OF NONHUMAN PRIMATE ANTIGENS

非人灵长类抗原的免疫组织化学鉴定

• 批准号: 7958291
• 负责人: Shawn Patrick O'Neil
• 金额: $ 6.23万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958291
• 关键词: Acquired Immunodeficiency Syndrome; Antibodies; Antigens; Cells; Comparative Pathology; Computer Retrieval of Information on Scientific Projects Database; Development; Diagnostic; Experimental Pathology; Formalin; Freezing; Funding; Grant; Housing; Human; Immunohistochemistry; Infectious Agent; Institution; Macaca; New England; Paraffin Embedding; Pathology; Primates; Protocols documentation; Research; Research Personnel; Resources; Source; Testing; Tissues; United States National Institutes of Health; Work; base; improved; molecular pathology; nonhuman primate; research and development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to improve our diagnostic pathology capabilities, we systematically evaluate commercially available antibodies directed against human cells/tissues and infectious agents for application in the nonhuman primate species housed at the NEPRC. Antibodies are selected for potential applications in diagnostic and/or experimental pathology, and for reactivity in either snap-frozen or formalin-fixed, paraffin-embedded tissues. In addition, the Division of Comparative Pathology receives multiple requests each year to test antibodies and develop IHC protocols for investigators in our Division, other NEPRC Divisions, and collaborating investigators. Thus, research and development to identify antibodies for immunohistochemistry in nonhuman primates is an important activity of the Molecular Pathology Core, and has contributed to the development of a diverse repertoire of IHC antibodies that work consistently in macaque tissue. AIDS related.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 为了提高我们的诊断病理学能力，我们系统地评估了针对人类细胞/组织和感染因子的市售抗体，以应用于 NEPRC 饲养的非人类灵长类动物。 选择抗体用于诊断和/或实验病理学中的潜在应用，以及在速冻或福尔马林固定、石蜡包埋的组织中的反应性。 此外，比较病理学部门每年都会收到多项请求，要求我们部门、其他 NEPRC 部门和合作研究人员测试抗体并制定 IHC 方案。 因此，研究和开发在非人灵长类动物中鉴定免疫组织化学抗体是分子病理学核心的一项重要活动，并有助于开发在猕猴组织中持续发挥作用的多种 IHC 抗体。 艾滋病相关。