NCCAT: National Center for CryoEM Access and Training- Supplement for Screening

NCCAT：国家冷冻电镜访问和培训中心 - 筛查补充资料

• 批准号: 10601312
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 27.31万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10601312
• 关键词: Cryoelectron Microscopy; Ensure; Equipment; Funding; Goals; Macromolecular Complexes; Methods; Microscope; Modeling; New York; Preparation; Production; Resolution; Resources; Robot; Specimen; Structure; Support Groups; Time; Training; Training Programs; base; biological systems; career; detector; experience; particle; screening; screening services; skills; structural biology; supplemental screening

Project Summary/Abstract The National Center for CryoEM Access and Training (NCCAT), based at the New York Structural Biology Center, provides access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team provides direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. A cross-training program provides training to users across a wide variety of skill levels and career goals. This is a request for supplemental funding to support a grid preparation and screening (GPS) service for cryo- TEM at NCCAT. The objective is to provide urgently needed support to groups who have limited experience with the methods of cryoEM and limited access to local facilities. The goal will be to support these groups to get them started while also training them in all possible aspects so that they can make best possible use of every resource available to them as soon as possible.

项目概要/摘要 国家冷冻电镜访问和培训中心 (NCCAT) 位于纽约结构生物学中心， 提供最先进的设备，包括标本制备机器人、高端显微镜 以及直接探测器、筛选显微镜以及解决结构问题所需的所有其他辅助设备 使用冷冻电子显微镜 (cryoEM) 方法获得尽可能高的分辨率。拥有一支高素质的技术队伍 团队提供直接支持、指导和帮助，以确保最高质量的结构 在最短的时间内生产出来。交叉培训计划为各种用户提供培训 技能水平和职业目标。 这是一项补充资金请求，以支持冷冻的网格准备和筛选（GPS）服务 NCCAT 的 TEM。目标是为经验有限的群体提供急需的支持 使用冷冻电镜方法和有限的当地设施。目标是支持这些团体 让他们开始，同时在所有可能的方面对他们进行培训，以便他们能够最好地利用 尽快为他们提供所有可用资源。