The National Center for In-situ Tomographic Ultramicroscopy (NCITU)

国家原位断层超显微术中心 (NCITU)

• 批准号: 10397367
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 24.66万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10397367
• 关键词: Cells; Electron Microscopy; Ensure; Equipment; Funding; Goals; In Situ; Liquid substance; Longevity; Methods; New York; Nitrogen; Preparation; Rod; Sampling; Services; Specimen; Time; Training; Training Programs; Vacuum; Visualization; base; career; design; method development; skills; structural biology; tomography; water vapor

Summary We propose to establish the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to be based at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCITU is dedicated to developing and providing access to both standard and advanced methods for in-situ cryoET specimen preparation, and to disseminating this expertise through a cryoET cross-training program designed to serve a wide variety of skill levels and career goals. The primary goal of the National Center for In-situ Tomographic Ultramicroscopy (NCITU) is to provide user access and service towards preparing specimen for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team provides direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. An already well established cross-training program is being expanded and extended to provide training in every aspect of the cryoET specimen preparation pipeline. This is a request for supplemental funding for the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to enhance the quality and throughput of the TFS Aquilos 2 cryo-FIB-SEM. This is a specific request for (i) a high-vacuum sample transfer rod, (ii) an in-chamber water vapor reduction upgrade, and (iii) a liquid nitrogen cooling longevity upgrade.

概括 我们建议建立国家原位层析成像超显镜（NCITU）的国家中心 Simons电子显微镜中心（SEMC），它是纽约结构生物学中心的一部分 （NYSBC），总部位于纽约，纽约。 NCITU致力于开发和提供对标准和访问的访问 原位冷冻标本准备的高级方法，并通过冷冻散发此专业知识 跨训练计划旨在服务于各种技能水平和职业目标。 国家原位断层扫描超显镜（NCITU）的主要目标是为用户提供 访问和服务，用于准备标本以直接可视化大分子关联和 组织，特别是在本地细胞中。一个高素质的技术团队提供直接的动手服务， 指导和协助，以确保以最高质量和中的样品和网格准备 最短的时间。一个已经建立的跨训练计划正在扩展并扩展到 在冷冻标本制备管道的各个方面提供培训。 这是国家原位断层扫描超显微镜中心补充资金的要求 （NCITU）增强TFS Aquilos 2 Cryo-Fib-Sem的质量和吞吐量。这是一个特定的请求 对于（i）高空VaCuum样品转移杆，（ii）培训内水蒸气还原升级和（iii）液体 氮冷却寿命升级。