NCITU: National Center for In-situ Tomographic Ultramicroscopy. Supplement

NCITU：国家原位断层显微术中心。

• 批准号: 10579763
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 24.24万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10579763
• 关键词: Cells; Computer software; Data Analyses; Electron Microscopy; Ensure; Equipment; Funding; Goals; In Situ; Methods; New York; Preparation; Sampling; Services; Specimen; Time; Training; Training Programs; Visualization; base; career; design; method development; skills; structural biology; tomography

Summary The National Center for In-situ Tomographic Ultramicroscopy (NCITU) is based at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCITU is dedicated to developing and providing access to both standard and advanced methods for in-situ cryoET specimen preparation, and to disseminating this expertise through a cryoET cross-training program designed to serve a wide variety of skill levels and career goals. The primary goal of the National Center for In-situ Tomographic Ultramicroscopy (NCITU) is to provide user access and service towards preparing specimen for direct visualization of macromolecular associations and organization, particularly in native cells. A highly qualified technical team provides direct hands-on service, guidance, and assistance to ensure that samples and grids are prepared with the highest quality and in the shortest time possible. An already well established cross-training program is being expanded and extended to provide training in every aspect of the cryoET specimen preparation pipeline. This is a request for supplemental funding for the National Center for In-situ Tomographic Ultramicroscopy (NCITU) to enhance the Center's data analysis and CLEM capabilities. This is a specific request for (i) a dedicated cryoET processing workstation, (ii) a Zeiss LSM 900 software upgrade, and (iii) a second Linkam CMS196 cryo CLEM stage.

概括 国家原位断层扫描超显镜（NCITU）的国家中心位于Simons Electron 显微镜中心（SEMC）是纽约结构生物学中心（NYSBC）的一部分，该中心位于新的 约克，纽约。 NCITU致力于开发和提供对标准和高级方法的访问 原位冷冻标本准备，并通过冷冻交叉训练传播此专业知识 计划旨在服务于各种技能水平和职业目标。 国家原位断层扫描超显镜（NCITU）的主要目标是为用户提供 访问和服务，用于准备标本以直接可视化大分子关联和 组织，特别是在本地细胞中。一个高素质的技术团队提供直接的动手服务， 指导和协助，以确保以最高质量和中的样品和网格准备 最短的时间。一个已经建立的跨训练计划正在扩展并扩展到 在冷冻标本制备管道的各个方面提供培训。 这是国家原位断层扫描超显微镜中心补充资金的要求 （NCITU）增强中心的数据分析和CLEM功能。这是（i） 专用的冷冻处理工作站，（ii）Zeiss LSM 900软件升级，以及（iii）第二个Linkam CMS196冷冻CLEM阶段。