NCCAT: National Center for CryoEM Access and Training

NCCAT：国家冷冻电镜访问和培训中心

• 批准号: 9924576
• 负责人: BRIDGET Olivia CARRAGHER
• 金额: $ 1234.24万
• 依托单位: NEW YORK STRUCTURAL BIOLOGY CENTER
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9924576
• 关键词: Adopted; Area; Cryoelectron Microscopy; Custom; Data; Data Collection; Derivation procedure; Educational Status; Educational workshop; Electron Microscopy; Ensure; Environment; Equipment; Generations; Goals; Human Resources; Immersion; Infrastructure; Intention; Laboratory Research; Macromolecular Complexes; Maps; Measures; Methods; Microscope; Modeling; New York; Phase; Positioning Attribute; Preparation; Process; Production; Quality Control; Records; Research Personnel; Resolution; Resources; Robot; Running; Sampling; Science; Services; Site; Specimen; Structure; System; Time; Titan; Training; Training Activity; Training Programs; Training and Infrastructure; base; biological systems; career; computerized data processing; data acquisition; density; detector; expectation; image processing; innovation; instrumentation; operation; particle; reconstruction; screening; skills; structural biology; tomography

Summary We propose to establish the National Center for CryoEM Access and Training (NCCAT) to be housed at the Simons Electron Microscopy Center (SEMC), which is a part of the New York Structural Biology Center (NYSBC), based in New York, NY. NCCAT will provide access to state-of-the-art equipment, including specimen preparation robots, high-end microscopes and direct detectors, screening microscopes, and all the other ancillary equipment required to solve structures to the highest possible resolution using cryo electron microscopy (cryoEM) methods. A highly qualified technical team will provide direct support, guidance and assistance to ensure that structures of the highest quality are produced in the shortest possible time. An already well established cross-training program will be expanded and extended to provide training across a wide variety of skill levels and career goals. NCCAT will adopt an open and transparent application process to ensure equal-opportunity nationwide access. The operations at NCCAT will build on already established practices at SEMC/NYSBC and benefit from the overall vibrant environment as well as the expertise of many of the highly trained staff already in place. NCCAT will be housed in new, custom-built space that will provide optimal, environmentally stable conditions. The major instrumentation will consist of 3 Titan Krios TEMs equipped with direct detectors, energy filters and phase plates, and 3 screening microscopes equipped with CMOS cameras. Streamlined workflows, already well established, will expanded to ensure that we collect the highest quality data as efficiently as possible. A user service operation will provide on-site, remote-access, and mail-in data collection services with fair and equal access to a nationwide user base. We will guide and assist users with all aspects of cryoEM, including sample preparation, data collection and image processing. A training program will encompass a full range of expectations, from the basics required to ensure that users produce valid structures, through more detailed training for users seeking independence, to fully embedded researchers who will be expected to graduate to running their own cryoEM research laboratories. While we anticipate that we will initially be called to serve and train mostly in the area of single particle cryoEM, we are well positioned to provide support in the area of cryoET that we predict will grow rapidly. While the goals of NCCAT are not innately innovative it is our intention to set the standard as one of the first world class, nationally accessible cryoEM access and training facilities that is freely and fairly available to all researchers in the US, and to continually strive to enable science of the highest quality and value.

概括 我们建议建立国家冷冻访问和培训中心（NCCAT） Simons电子显微镜中心（SEMC），该中心是纽约结构生物学中心的一部分 （NYSBC），总部位于纽约，纽约。 NCCAT将提供使用最新设备的访问 标本准备机器人，高端显微镜和直接检测器，筛选显微镜以及所有 使用冷冻电子将结构求解为最高分辨率所需的其他辅助设备 显微镜（冷冻）方法。高素质的技术团队将提供直接的支持，指导和 帮助确保最高质量的结构在最短的时间内产生。一个 已经建立了良好的跨训练计划将进行扩展和扩展，以提供跨越的培训 各种各样的技能水平和职业目标。 NCCAT将采用开放透明的申请流程 确保全国范围内的同等运动。 NCCAT的运营将基于SEMC/NYSBC已经建立的实践，并从中受益 总体充满活力的环境以及许多训练有素的员工的专业知识已经到位。 NCCAT 将安置在新的定制空间中，将提供最佳的环境稳定条件。这 主要的仪器将由配备直接探测器，能量过滤器和 相板和3个配备CMOS摄像机的筛选显微镜。已经简化了工作流程 建立良好的人将扩大，以确保我们尽可能有效地收集最高质量的数据。一个 用户服务操作将通过公平和 同等地访问全国用户群。我们将指导和协助用户制作冷冻的各个方面，包括 样本准备，数据收集和图像处理。培训计划将包括 期望，从确保用户生成有效结构的基础知识，通过更详细的 培训寻求独立的用户，以完全嵌入的研究人员的培训 运行自己的冷冻研究实验室。尽管我们预计我们最初将被要求服务，并且 我们大多在单个粒子冷冻的区域训练，我们在 我们预测的冷冻物将迅速增长。 尽管NCCAT的目标不是天生的创新，但我们打算将标准设定为第一个标准之一 世界一流，全国访问的冷冻访问和培训设施，可自由且公平地为所有人使用 美国的研究人员，并不断努力实现最高质量和价值的科学。