Metabolic Phenotyping in Live Models of Obesity and Diabetes

肥胖和糖尿病活体模型的代谢表型分析

基本信息

批准号：
10588794
负责人：
Carol Fuzeti Elias
金额：
$ 74.1万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-02-01 至 2028-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588794
关键词：
Accounting Advertising Animal Experiments Animal Model Animals Anxiety Area Basic Science Behavior assessment Behavioral Biological Biomedical Research Blood specimen Cannulations Chronic Circadian Dysregulation Consultations Core Facility Culex (Genus)Data Databases Development Diabetes Mellitus Educational workshop Experimental Designs Fiber Fostering Funding Genetic Germ-Free Glucose Glucose Clamp Goals Grant Health Health Care Costs Health system Homeostasis Hormone secretion Hormones Housing Human Hyperinsulinism Hypothalamic structure Indirect Calorimetry Individual Indwelling Catheter Institution Investments Laboratories Leadership Learning Lipids Liquid substance Medicine Mental Depression Metabolic Metabolic Diseases Metabolism Michigan Modeling Morbidity - disease rate Motor Activity Mus National Institute of Diabetes and Digestive and Kidney Diseases Obesity Operant Conditioning Operative Surgical Procedures Parabiosis Pathogenesis Pattern Phenotype Photometry Physical activity Physiological Physiology Portal vein structure Protocols documentation Reproducibility Research Research Personnel Resources Role Sampling Services Standardization Technology Temperature Testing Tracer Training Training Programs Translational Research Transportation Universities animal care arm base behavior measurement behavioral phenotyping behavioral response blood glucose regulation brain surgery burden of illness comorbidity cost dietary experience feeding glucose tolerance hedonic improved in vivo insulin tolerance member metabolic phenotype mortality mouse genetics mouse model novel novel therapeutic intervention obesity treatment optogenetics outreach response tool

项目摘要

Project Summary / Abstract – Overall Metabolic disorders, including obesity, diabetes and their complications are among the most pressing health issues worldwide accounting for a substantial national burden of morbidity, mortality, and healthcare costs. The University of Michigan Health System (now Michigan Medicine) has strategically invested in both human and physical resources over the past 20 years in a concerted attack on these problems. A key component of this strategy is to foster the development of core laboratories providing expertise and specialized services at accessible cost to researchers throughout the institution. The University of Michigan is also a member of the national consortium established in 2016 by the NIDDK for in-depth phenotyping tests of mouse models of obesity, diabetes, and related metabolic conditions, the Mouse Metabolic Phenotyping Centers (MMPC). The Michigan Metabolic Phenotyping in Live Models of Obesity and Diabetes (MPMOD) will build upon our stablished expertise to provide a unique pallet of standardized, high quality and state-of-the-art in vivo phenotyping services for researchers nationwide with focus on underrepresented PIs and small laboratories or institutions that have not received significant federal research funding. The MPMOD at Michigan will be organized in three distinct cores: The Administrative Core, the Animal Care and Germ-Free Mouse Core, and the Metabolic, Physiological and Behavioral (MPB) Phenotyping Core. The Administrative Core will provide overall scientific and fiscal leadership. It will coordinate phenotyping activities among the individual cores and will foster interaction with phenotyping centers members of the MPMOD Consortium and the Coordinating Unit (CU). The Animal Care and Germ-Free Mouse Core will be responsible for transportation, housing, and veterinary care of animals to be assessed at the MPB Phenotyping Core. The MPB Phenotyping Core will provide a comprehensive battery of in vivo assessments encompassing those provided by the former MMPC, i.e., glucose or lipid homeostasis (glucose tolerance, insulin tolerance, lipid tolerance, hyperinsulinemic- euglycemic clamps with tracers), whole-animal energy homeostasis (indirect calorimetry by CLAMS, SABLE, with dietary and environmental temperature challenges), ultradian hormone secretion (Culex platform for serial biological fluid sampling from free-moving mice without human presence), behavioral measurements (e.g., hedonic and homeostatic feeding responses, and obesity- and/or diabetes-associated changes in learning, anxiety and depression), etc. Additional in vivo services using highly specialized surgical procedures (e.g., chronic portal vein cannulation, parabiosis, and stereotaxic brain surgeries), optogenetic and fiber photometry technologies. Together, the Michigan MPMOD will provide access to a broad range of advanced in vivo phenotyping services, consultation and training taking advantage of our well-stablished metabolic phenotyping facilities and unique expertise on hypothalamic function, mouse genetics and specialized surgeries in experimental animals.

项目摘要 /摘要 - 总体新陈代谢疾病，包括肥胖，糖尿病及其并发症是最紧迫的健康之一全球范围内的发病率，死亡率和医疗保健成本的大量燃烧。密歇根大学卫生系统（现为密歇根州医学）已战略性地投资于人类和在过去20年中，对这些问题进行了一致攻击。一个关键组成部分战略是为了促进提供专业知识和专业服务的核心实验室的发展整个机构的研究人员的可及成本。密歇根大学也是 NIDDK于2016年成立的国家财团，用于对小鼠模型的深入表型测试肥胖，糖尿病和相关代谢状况，小鼠代谢表型中心（MMPC）。肥胖和糖尿病现场模型中的密歇根州代谢表型（mpmod）将建立在我们的基础上稳固的专业知识，提供独特的体内标准化，高质量和最先进的托盘全国研究人员的表型服务专注于代表性不足的PI和小型实验室或尚未获得大量联邦研究资金的机构。密歇根州的mpmod将是以三种不同的核心组织：行政核心，动物护理和无菌小鼠核心，以及代谢，生理和行为（MPB）表型核心。行政核心将提供总体科学和财政领导。它将协调单个核心之间的表型活动将促进与MPMOD联盟和协调单元的表型中心成员的相互作用（Cu）。动物护理和无菌小鼠核心将负责运输，住房和在MPB表型核心评估动物的兽医护理。 MPB表型核心将提供全面的体内评估电池，包括前MMPC提供的评估即葡萄糖或脂质稳态（葡萄糖耐受性，胰岛素耐受性，脂质耐受性，高胰岛素血症）带有示踪剂的Eugycemic夹具），全动物的能量稳态（通过蛤，黑貂的间接量热法，饮食和环境温度挑战），超级激素分泌（串行的Culex平台来自不存在人类的自由移动小鼠的生物流体采样），行为测量（例如，享乐和稳态喂养反应，以及肥胖和/或与糖尿病相关的学习变化，焦虑和抑郁）等。使用高度专业的手术程序（例如，慢性门静脉插管，抛物线和立体定位脑外科手术），光遗传学和纤维光度法技术。密歇根州MPMod一起将提供广泛的高级体内访问权限表型服务，咨询和培训利用了我们稳固的代谢表型在下丘脑功能，小鼠遗传学和专业手术方面的设施和独特的专业知识实验动物。