Michigan Mouse Metabolic Phenotyping Center

密歇根小鼠代谢表型中心

基本信息

批准号：
10411881
负责人：
Carol Fuzeti Elias
金额：
$ 54.35万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-02 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10411881
关键词：
16S ribosomal RNA sequencing Anaerobic Bacteria Animals Area Atherosclerosis Award Behavior Biochemical Bioinformatics Biological Biological Assay Biological Models Blindness Blood Pressure Blood Vessels Cardiac Cardiovascular Physiology Cardiovascular system Caring Clinical Chemistry Collaborations Complications of Diabetes Mellitus Culex (Genus)Data Data Set Databases Development Diabetes Mellitus Diabetic Angiopathies Disease Echocardiography Educational workshop Electrocardiogram Endocrine Exercise Tolerance Fostering Functional disorder Funding Gastrectomy Generations Genes Genomics Germ-Free Glucose Glucose Clamp Gnotobiotic Goals Grant Health Care Costs Healthcare Heart failure Histopathology Homeostasis Hormone secretion Housing Human Image In Vitro Indirect Calorimetry Institution Kidney Diseases Kidney Failure Laboratories Leadership Libraries Liquid substance Literature Maintenance Measurement Metabolic Metabolic Diseases Metabolism Michigan Microvascular Dysfunction Modeling Motor Activity Mouse Strains Mus Myocardial Ischemia Neuropathy Obesity Operant Conditioning Pathogenesis Pattern Phenotype Physiological Physiology Protocols documentation Publishing Reproducibility Research Research Personnel Resources Retinal Diseases Rewards Ribosomes Sampling Sensitivity and Specificity Services Surgical Models Telemetry Testing Tissues Training Programs Ultrasonography Universities Wild Type Mouse animal care bariatric surgery behavior measurement biobehavior blood glucose regulation burden of illness cost diabetic dietary experience glucose tolerance gut microbiome human model in vivo insulin tolerance knowledge base macrovascular disease medical schools member metabolic phenotype microbial microbial genome microbiome microbiome analysis mouse model nerve damage novel operation phenotypic data pressure programs tool training opportunity transcriptome sequencing transcriptomics transmission process

项目摘要

Project Summary / Abstract – Overall Metabolic disorders and their vascular complications are among the most pressing healthcare issues worldwide. The University of Michigan Medical School has strategically invested in both human and physical resources over the past 15 years under the umbrella of the Michigan Comprehensive Diabetes Center in a concerted attack on this problem. One component of the strategy has been to foster the development of core laboratories within the university to provide key expertise and specialized phenotyping services at reasonable cost to researchers throughout the institution. We now propose to coalesce a quartet of these phenotyping cores into the Michigan MMPC to continue providing comprehensive and state of the art services internally, while expanding their availability to researchers on a national level, and continuing the development of more sensitive and more specific tools to probe the pathogenesis and biochemical consequences of diabetes and obesity in mouse models. The Michigan MMPC will provide services in four major areas. The Metabolism, Bariatric Surgery and Behavior Core will perform a variety of in vivo physiological assessments encompassing glucose homeostasis (glucose tolerance, insulin tolerance, hyperinsulinemic/euglycemic clamps), energy homeostasis (indirect calorimetry by CLAMS, dietary challenge), ultradian hormone secretion (Culex platform for serial biological fluid sampling from unrestrained mice), behavioral measurements (locomotor activity, meal pattern analysis, operant conditioning) and generation of bariatric surgery models. The Cardiovascular Pathophysiology Core will assess cardiovascular function (exercise tolerance, blood pressure, ECG, telemetry, echocardiogram), offer mouse surgical models of cardiac ischemia and pressure overload and imaging using ultrasound. The Microvascular Complications Core will provide basic and advanced assessment of diabetes complications including neuropathy, nephropathy, and retinopathy. The Microbiome Core will provide anaerobic microbial culture, microbiome genomic and transcriptomic libraries, 16S ribosomal gene and RNA sequencing with associated bioinformatic analysis. Mouse importation, housing, veterinary care, clinical chemistry and histopathology services will be provided by the Animal Care Core. In addition, the latter core hosts a germ-free mouse facility and will interact with the Microbiome core to produce and distribute germ-free and gnotobiotic mouse models. The Administrative Core will manage all Center activities, oversee financial operations, provide training opportunities, and collaborate with the other phenotyping centers and the Central Coordinating and Bioinformatics Unit of the MMPC Consortium by providing all collected phenotyping data and protocols and participating in the Pilot and Feasibility award and MICROMouse funding programs. Together, our Core resources will provide in a single Center access to a broad range of advanced phenotyping capabilities, including our unique focus and expertise on microvascular complications, microbiome analysis and germ-free mice and bariatric surgery models.

项目总结/摘要——总体代谢紊乱及其血管并发症是最紧迫的医疗保健问题之一密歇根大学医学院在全球范围内对人类和身体进行了战略投资。过去 15 年在密歇根综合糖尿病中心的保护下的资源该战略的一个组成部分就是促进核心技术的发展。大学内的实验室以合理的价格提供关键专业知识和专业表型服务我们现在建议合并这些表型分析的四个部分。核心进入密歇根 MMPC，继续在内部提供全面且最先进的服务，同时在全国范围内扩大研究人员的可用性，并继续开发更多敏感和更具体的工具来探讨糖尿病的发病机制和生化后果密歇根 MMPC 将在代谢、肥胖等四个主要领域提供服务。减肥手术和行为核心将进行各种体内生理评估，包括葡萄糖稳态（葡萄糖耐量、胰岛素耐量、高胰岛素/正常血糖钳夹）、能量体内平衡（通过 CLAMS 间接测热、饮食挑战）、超电激素分泌（Culex 平台用于从不受约束的小鼠中连续采集生物液体样本）、行为测量（运动活动、进餐模式分析、操作条件反射）和减肥手术模型的生成。病理生理学核心将评估心血管功能（运动耐量、血压、心电图、遥测、超声心动图），提供心脏缺血和压力超负荷的小鼠手术模型以及使用成像微血管并发症核心将提供糖尿病的基本和高级评估。微生物组核心将提供包括神经病、肾病和视网膜病在内的并发症。厌氧微生物培养、微生物基因组和转录组文库、16S 核糖体基因和 RNA 测序及相关的生物信息学分析。小鼠输入、饲养、兽医护理、临床。此外，动物护理核心将提供化学和组织病理学服务。拥有无菌小鼠设施，并将与微生物组核心互动以生产和分销无菌产品行政核心将管理中心的所有活动，监督财务。操作，提供培训机会，并与其他表型中心和中央合作 MMPC 联盟的协调和生物信息学单位提供所有收集的表型数据和协议并参与试点和可行性奖以及 MICROMuse 资助计划。我们的核心资源将在一个中心提供广泛的高级表型分析能力，包括我们对微血管并发症、微生物组分析的独特关注和专业知识以及无菌小鼠和减肥手术模型。