MIRA: The biological basis of paternal care in stickleback fish

MIRA：刺鱼父系照顾的生物学基础

基本信息

批准号：
10582361
负责人：
Alison Marie Bell
金额：
$ 3.29万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-01-05 至 2025-12-31
项目状态：
未结题

项目摘要

PROJECT SUMMARY/ABSTRACT Parental care is critical to the health of both parents and offspring, yet little is known about how molecular substrates act within brain areas and neuronal circuits to alter parental behavior, and this gap is especially wide for paternal care, i.e. the care-giving behavior of fathers. The proposed research capitalizes on natural variation in paternal care within a single species (threespined stickleback fish) to uncover the biological basis of paternal care in vertebrates. The current models for paternal care have biparental care, which makes it difficult to disentangle the behavior of mothers and fathers. Threespined stickleback fish are a promising new model for paternal care because fathers are solely responsible for providing care. Moreover, the molecular mechanisms related to care are deeply conserved in vertebrates, the stickleback genome is small and compact and there are a growing number of functional tools available to study them. The goals of this proposal are to define the molecular elements responsible for differences in paternal care within a single species and to characterize the neurobiological pathways involved in such variation in behavior. There are two objectives: 1) Identify genes that contribute to differences in paternal care and test their function; 2) Define the ways in which neuropeptide signaling regulates paternal care. The project combines brain gene expression profiling at the bulk and single cell level, viral mediated transgenesis experiments to establish mechanism and pharmacological manipulations of oxytocin and arginine vasopressin signaling. The proposed work offers an innovative solution to the challenge of dissecting the biological basis of paternal care by using a powerful new model for fathering, and by capitalizing on natural variation in paternal care within a single species.

项目摘要/摘要父母的护理对父母和后代的健康至关重要，但对分子的方式知之甚少底物在大脑区域和神经元电路内作用以改变父母的行为，尤其是这种差距宽阔的父亲护理，即父亲的照料行为。拟议的研究资源是自然的单个物种内的父亲护理的变化（三杆粘鱼鱼）以发现生物学基础脊椎动物的父亲护理。当前的父亲护理模型具有双方护理，这使得很难解开母亲和父亲的行为。三杆粘鱼是一种有希望的新的父亲护理的模型是因为父亲完全负责提供护理。而且，分子与护理有关的机制在脊椎动物中深处保守，粘性基因组很小且紧凑并且有越来越多的功能工具可用于研究它们。该提议的目标是定义负责单个物种内父亲护理差异的分子元素和表征与这种行为变化有关的神经生物学途径。有两个目标：1）确定导致父亲护理差异并测试其功能的基因； 2）定义的方式神经肽信号传导调节父亲护理。该项目结合了大脑基因表达分析散装和单细胞水平，病毒介导的转基因实验，以建立机制和催产素和精氨酸加压素信号传导的药理操作。拟议的工作提供了创新的解决方案，通过使用强大的新型新的挑战，以剖析父亲护理的生物学基础育种模型，并通过利用单个物种内的父亲护理的自然变异。