MIRA: The biological basis of paternal care in stickleback fish

MIRA：刺鱼父系照顾的生物学基础

基本信息

批准号：
10543160
负责人：
Alison Marie Bell
金额：
$ 38.56万
依托单位：
UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-01-05 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10543160
关键词：
Affect Affective Amygdaloid structure Area Argipressin Behavior Behavioral Biological Biology Brain Brain region California Callithrix Caring Cells Child Compensation Decision Making Elements Exhibits Family Fathers Fishes Gasterosteidae Gene Expression Profiling Gene Transfer Techniques Genes Genetic Genome Genomics Goals Health Human Hypothalamic structure Lateral Maternal Behavior Medial Mediating Microtus Modeling Modernization Molecular Mothers Mus Neurobiology Neurons Neuropeptides Oxytocin Parents Pathway interactions Peromyscus Play Preoptic Areas Provider Recommendation Research Signal Transduction Societies Study models System Testing Text Variant Vertebral column Vertebrates Viral Work brain circuitry caregiving cell type cognitive development experimental study fascinate innovation model organism neuronal circuitry offspring pharmacologic prairie vole social tool

项目摘要

Enter the text here that is the new abstract information for your application. This section must be no longer than 30 lines of text. The Abstract is unchanged. Parental care is critical to the health of both parents and offspring, yet little is known about how molecular substrates act within brain areas and neuronal circuits to alter parental behavior, and this gap is especially wide for paternal care, i.e. the care-giving behavior of fathers. The proposed research capitalizes on natural variation in paternal care within a single species (threespined stickleback fish) to uncover the biological basis of paternal care in vertebrates. The current models for paternal care have biparental care, which makes it difficult to disentangle the behavior of mothers and fathers. Three-spined stickleback fish are a promising new model for paternal care because fathers are solely responsible for providing care. Moreover, the molecular mechanisms related to care are deeply conserved in vertebrates, the stickleback genome is small and compact and there are a growing number of functional tools available to study them. The goals of this proposal are to define the molecular elements responsible for differences in paternal care within a single species and to characterize the neurobiological pathways involved in such variation in behavior. There are two objectives: 1) Identify genes that contribute to differences in paternal care and test their function; 2) Define the ways in which neuropeptide signaling regulates paternal care. The project combines brain gene expression profiling at the bulk and single cell level, viral mediated transgenesis experiments to establish mechanism and pharmacological manipulations of oxytocin and arginine vasopressin signaling. The proposed work offers an innovative solution to the challenge of dissecting the biological basis of paternal care by using a powerful new model for fathering, and by capitalizing on natural variation in paternal care within a single species.

在此处输入文本，这是您应用程序的新摘要信息。本节必须不超过30行文本。摘要没有变化。父母的护理对父母和后代的健康至关重要，但是对于分子底物如何在大脑区域和神经元回路中起作用以改变父母行为的知识知之甚少，对于父亲护理，即父亲的护理行为，这一差距尤为广泛。拟议的研究利用了单个物种（三杆粘鱼鱼）内父亲护理的自然变异，以揭示脊椎动物中父亲护理的生物学基础。当前的父亲护理模型具有二元护理，这使得很难解除母亲和父亲的行为。三尖粘鱼是父亲护理的有前途的新模式，因为父亲完全负责提供护理。此外，与护理有关的分子机制在脊椎动物中是深处的，粘性基因组小巧紧凑，并且可以使用越来越多的功能工具来研究它们。该提案的目标是定义负责单个物种内父亲护理差异的分子元素，并表征与这种行为变化有关的神经生物学途径。有两个目标：1）确定导致父亲护理差异并测试其功能的基因； 2）定义神经肽信号传导调节父亲护理的方式。该项目结合了大脑基因表达分析在整体和单细胞水平，病毒介导的转基因实验，以建立催产素和精氨酸加压素信号传导的机理和药理操作。拟议中的工作为通过使用强大的父母模型并利用单个物种内的父亲护理的自然变异来阐明父亲护理生物护理生物学的挑战提供了创新的解决方案。