Epigenetic mechanisms and consequences of fathering in sticklebacks

刺鱼的表观遗传机制和父亲的后果

• 批准号: 9146911
• 负责人: Alison Marie Bell
• 金额: $ 33.68万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9146911
• 关键词: Adoption; Affect; Animal Model; Attention; Behavior; Behavioral; Behavioral Mechanisms; Biological Models; Brain; Caring; DNMT3a; Data; Development; Environment; Epigenetic Process; Family; Fathers; Female; Fishes; Gasterosteidae; Gene Expression; Generations; Genes; Genome; Goals; Health; Health behavior; Human; Individual; Life; Link; Mediating; Mission; Modeling; Modification; Molecular; Mothers; Organism; Outcome; Parents; Paternal Behavior; Pathway interactions; Personal Satisfaction; Physiology; Provider; Public Health; Research; Shapes; Social Behavior; Social Environment; Stress; System; Time; Variant; Work; base; behavior influence; externalizing behavior; genome sequencing; improved; innovation; male; offspring; protective effect; success; therapy development

 DESCRIPTION (provided by applicant): Our understanding of fathering has lagged far behind our understanding of mothering, yet fathers have a substantial impact on the well-being of their offspring. The overall objective of this application is to understand the behavioral, molecular and epigenetic mechanisms that contribute to the effects of fathering on offspring. The central hypothesis is that fathering, like mothering, has long-lasting, transgenerational consequences for offspring behavior that are mediated via epigenetic changes in the genome that influence brain gene expression. The rationale that underlies the proposed research is that studying natural variation in social behavior in a tractable vertebrate system with sophisticated paternal behaviors is likely to have success identifying the causes and consequences of fathering in other organisms, including humans. There are two specific aims: 1: Identify the sensitive period and the molecular mechanisms underlying the effect of fathering on offspring. The working hypothesis for this aim is that fathering influences offspring gene expression during a sensitive period in development that has consequences for offspring behavior later in life. 2: Identify the epigenetic modifications caused by fathering. The working hypothesis for this aim is that fathering causes epigenetic modifications to the genome that influence offspring brain gene expression, behavior and stress physiology. The expected outcome of this project is a detailed understanding of the basic mechanisms by which family environments created by different fathering styles exert protective effects on offspring. These outcomes are expected to have an important positive impact because understanding the impacts of fathers on offspring could enhance the development of interventions that promote the adoption of healthy behaviors. The contribution of the proposed research is expected to be a detailed understanding of the behavioral, molecular and epigenetic mechanisms by which fathers influence their offspring using a new model system for fathering. This contribution will be significant because it will reveal if fathering, like mothering, can influence subsequent generations via epigenetic modifications to the genome. The proposed research is innovative because it utilizes a new genomically-enabled model organism in which fathers are the sole providers of parental care (threespined stickleback fish). The benefit of using a system with sole paternal care such as sticklebacks is that we can isolate the effects of fathering from the effects of mothering.

 描述（由应用程序提供）：我们对父亲的理解远远落后于我们对母亲的理解，但是父亲对他们后代的福祉产生了重大影响。该应用的总体目的是了解行为，分子和 有助于父亲对后代的影响的表观遗传机制。中心假设是，像母亲一样，父亲对后代行为具有长期的变革后果，这些后代是通过影响脑基因表达的基因组的表观遗传变化而介导的。拟议研究基础的基本原理是，研究具有复杂的父亲行为的可寓言脊椎动物系统中社会行为的自然变化很可能成功地识别了包括人类在内的其他生物体中父亲的原因和后果。有两个具体的目的：1：确定父亲对后代作用的敏感时期和分子机制。这个目标的工作假设是，父亲在发育敏感时期影响后代基因的表达，这对后代后代的后代行为产生了影响。 2：确定父亲引起的表观遗传修饰。这个目的的工作假设是，父亲对影响后代脑基因表达，行为和压力生理的基因组引起表观遗传修饰。该项目的预期结果是对不同父亲风格创造的家庭环境对后代的保护作用的基本机制的详细理解。预计这些结果将产生重要的积极影响，因为了解父亲对后代的影响可以增强促进采用健康行为的干预措施的发展。拟议研究的贡献预计将是对父亲使用新的父亲模型系统影响其后代的行为，分子和表观遗传机制的详细理解。这项贡献将是重要的，因为它将揭示父亲是否像母亲一样可以通过对基因组的表观遗传学修改影响后代。拟议的研究具有创新性，因为它利用了一种新的基因组模型生物体，在该模型中，父亲是父母护理的唯一提供者（三杆粘背鱼）。使用具有唯一父亲护理的系统（例如Sticklebacks）的好处是，我们可以将父亲的效果隔离出来。