Pre-Clinical Evaluation Core

临床前评估核心

基本信息

批准号：
10579204
负责人：
MANDI J. LOPEZ
金额：
$ 33.64万
依托单位：
LOUISIANA STATE UNIV A&M COL BATON ROUGE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-03-01 至 2026-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10579204
关键词：
3-Dimensional 3D Print Address Animal Cancer Model Animal Diseases Animal Model Antineoplastic Agents Apoptosis Biological Biological Assay Biology CRISPR/Cas technology Cancer Model Cell Culture System Cell Culture Techniques Cell Proliferation Cell Separation Cells Centers of Research Excellence Chronic Complement Consult Consultations Core Facility Data Analyses Development Disease Drug Kinetics Drug Screening Emerging Technologies Equipment Evaluation Exhibits Experimental Designs Extracellular Matrix Faculty Gene Expression Goals Human Human Cell Line Image Immunohistochemistry Immunologics In Vitro Lung Malignant Neoplasms Malignant neoplasm of liver Malignant neoplasm of prostate Measures Modeling Molecular Biology Mus Mutagenesis Outcome Pathology Patient-Focused Outcomes Patients Pharmacotherapy Population Postdoctoral Fellow Pre-Clinical Model Progressive Disease Reagent Regenerative Medicine Research Research Personnel Resource Development Resources Services Signal Pathway Structure System Technology Therapeutic Toxic effect Toxicology Training Training and Education Translational Research Update Validation anticancer research bioprinting cancer cell clinically relevant experience genetic manipulation graduate student human disease human model in vivo in vivo Model insight instrumentation malignant breast neoplasm mouse model novel therapeutic intervention novel therapeutics osteosarcoma pre-clinical preclinical evaluation scaffold screening three dimensional cell culture translational cancer research translational therapeutics tumor tumor microenvironment tumorigenesis

项目摘要

Summary Pre-Clinical Evaluation Core. The Center for Pre-Clinical Cancer Research (Cancer COBRE) aims to identify clinically relevant mechanisms of human cancer using models that closely reflect the disease state in the context of the tumor microenvironment to reveal insights into tumorigenesis and thus drive novel therapeutic discovery. Promising Junior Investigators (PJIs) address devastating and/or chronic human diseases that exhibit poor outcomes in patients and for which there are unmet therapeutic needs. The four PJI projects feature osteosarcoma, breast cancer, liver cancer, and prostate cancer as diseases that would benefit from pre-clinical models that are more predictive of mechanistic efficacy in humans. The PJI projects will use specialized in vivo mouse models and/or in vitro 3D tumor spheroids to identify or validate relevant mechanisms of tumorigenesis or for evaluation of novel therapeutic strategies. The Center will promote a unified and coordinated approach by establishing a new specialized core facility, Pre-Clinical Evaluation Core (PCEC), to provide scientific expertise and technical support for the pre-clinical evaluation of the PJI projects. Human and animal diseases are a major focus at the LSU SVM and on the LSU campus. The unique PCEC will provide a centralized approach that will support exploratory, as well as translational assessment of cancer disease mechanisms and therapeutic strategies in humans. To accomplish this approach, advanced 3D cancer cell culture systems (spheroids) will provide critical translational information. The Center has three Aims: Aim 1. To centralize management and coordination of pre-clinical evaluation of tumorigenesis diseases mechanisms and translational therapeutic approaches. Aim 2. To provide the COBRE PJIs and other biomedical investigators with resources for development and analysis of advanced preclinical cancer models, including 3D bioprinting and cell culture training, instrumentation, technical support, and consultation. Aim 3. To educate faculty, staff, postdoctoral fellows, and graduate students regarding pre-clinical evaluation using state-of-the-art in vitro 3D cell cultures to complement in vivo models.

临床前评估核心摘要。临床前癌症研究中心 (Cancer COBRE) 旨在临床识别使用密切反映疾病状态的模型来研究人类癌症的相关机制肿瘤微环境的背景，以揭示肿瘤发生的见解，从而推动新的研究治疗发现。有前途的初级研究者 (PJI) 致力于解决破坏性和/或慢性人类疾病对患者的治疗效果不佳，且治疗方法尚未得到满足治疗需求。四个 PJI 项目涉及骨肉瘤、乳腺癌、肝癌和前列腺癌作为疾病将受益于更具预测性的临床前模型人类的机械功效。 PJI 项目将使用专门的体内小鼠模型和/或体外 3D 肿瘤球体，以识别或验证肿瘤发生的相关机制或用于评估新的治疗策略。中心将推动统一协调通过建立新的专业核心设施，临床前评估核心（PCEC），为PJI的临床前评价提供科学知识和技术支持项目。人类和动物疾病是路易斯安那州立大学 SVM 和路易斯安那州立大学校园的主要关注点。独特的 PCEC 将提供一种集中式方法，支持探索性以及人类癌症疾病机制和治疗策略的转化评估。为了实现这一方法，先进的 3D 癌细胞培养系统（球体）将提供关键翻译信息。该中心有三个目标：目标 1. 集中管理肿瘤发生疾病机制的临床前评估和协调转化治疗方法。目标 2. 提供 COBRE PJI 和其他生物医学拥有开发和分析先进临床前癌症模型资源的研究人员，包括 3D 生物打印和细胞培养培训、仪器、技术支持和咨询。目标 3. 教育教职员工、博士后研究员和研究生关于使用最先进的体外 3D 细胞培养物进行临床前评估以补充体内模型。