Prevention of Hip Dysplasia with Thermal Energy

用热能预防髋关节发育不良

• 批准号: 6747585
• 负责人: MANDI J. LOPEZ
• 金额: $ 11.01万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6747585
• 关键词: articular cartilage; biomarker; biomechanics; chondroitin sulfates; collagen; computed axial tomography; disease /disorder prevention /control; dogs; enzyme linked immunosorbent assay; gene expression; hip; hyperthermia therapy; joint disorder; longitudinal animal study; mucopolysaccharides; nonhuman therapy evaluation; osteoarthritis; polymerase chain reaction; proteoglycan; radiography; radioimmunoassay; radiowave radiation; synovial fluid; viscosity

The fundamental goals of this research proposal are to test the hypotheses that: 1) application of thermal energy at 65 degrees C using radio-frequency energy to the coxofemoral joint capsule of puppies with radiographic evidence of joint laxity will result in normal hip congruity, 2) will prevent the development of the lameness and the cartilagenous and bony changes characteristic of CHD, and 3) will not have detrimental effects on the biomechanical or viscoelastic properties of the joint capsular tissue. These investigations will yield insights into the fundamental mechanisms of thermal modification of musculoskeletal soft tissue. This research program is designed to determine whether thermal modification of coxocofemoral joint capsule of 16-week-old puppies with excessive joint: laxity will prevent CHD. Ultimately, this model will serve to evaluate the potential of thermal energy to reduce excessively lax coxofemoral joints in neonatal children and thereby help prevent DDH. The candidate of this application graduated magna cum laude from Humboldt State University with a B.S. in cellular-molecular biology and a minor in chemistry. She worked as a molecular biologist at Amgen, Thousand Oaks, CA, before undertaking and; completing her D.V.M. at the University of California-Davis. Dr. Lopez was awarded 5 scholarships and 2 research grants while a D.V.M. student. She completed an internship at Kansas State University and a large animal surgery residency/Master's degree at they University of Wisconsin- Madison. She is a board certified large animal surgeon. Dr. Lopez's Master's thesis was entitled "Effects of Radio-frequency Energy on Ovine Stifle Joint Capsule", and she performed much of the early radio- frequency work in the Comparative Orthopaedic Research Laboratory. She was awarded an NRSA in 1998 and has performed the background research and preliminary, studies that form the basis for this proposal. She passed her preliminary examinations "With Honors" in November, 1999, and is scheduled to complete her PhD in June 2001. Her ultimate goal is to join the faculty of an academic institution as a well-trained scientist and productive researcher specializing in integrative, translational research. The training environment will be guided toward mentoring the candidate while teaching valuable research techniques necessary to begin an independent research career. The majority of supervision will be provided by Mark D. Markel, an internationally known and respected investigator in the area of thermal tissue modification, Professor of Surgery, and Professor and Chairman of Medical Sciences. The advisory committee will consist of well-respected and highly experienced individuals within the University. The Comparative Orthopaedic Research Laboratory is a well established laboratory that houses multi- disciplinary research programs. The University of Wisconsin provides an exceptional environment for interaction with experts in a variety of fields, and it is well known for its exceptional research programs, especially in the medical sciences.

本研究提案的基本目标是测试以下假设：1) 使用射频能量将 65 摄氏度的热能应用于具有关节松弛的放射学证据的幼犬的髋股关节囊，将导致正常的髋关节一致性，2 ) 将防止先天性心脏病 (CHD) 的跛行以及软骨和骨变化特征的发展，并且 3) 不会对生物力学或粘弹性特性产生有害影响关节囊组织。这些研究将深入了解肌肉骨骼软组织热改性的基本机制。该研究项目旨在确定对关节过度松弛的 16 周龄幼犬的髋股关节囊进行热改造是否可以预防冠心病。最终，该模型将用于评估热能减少新生儿过度松弛的髋股关节的潜力，从而有助于预防 DDH。此申请的候选人以优异的成绩毕业于洪堡州立大学，并获得了理学学士学位。主修细胞分子生物学，辅修化学。在从事以下工作之前，她曾在加利福尼亚州千橡市的安进公司担任分子生物学家；完成她的 D.V.M.在加州大学戴维斯分校。洛佩兹博士获得 5 项奖学金和 2 项研究补助金，同时获得 D.V.M.学生。她在堪萨斯州立大学完成了实习，并在威斯康星大学麦迪逊分校完成了大型动物外科住院医师实习/硕士学位。她是经过委员会认证的大型动物外科医生。洛佩兹博士的硕士论文题为“射频能量对羊膝关节囊的影响”，她在比较骨科研究实验室进行了大部分早期射频工作。她于 1998 年被授予 NRSA 并进行了背景研究和初步研究，这些研究构成了本提案的基础。她于 1999 年 11 月“以优异的成绩”通过了初步考试，并计划于 2001 年 6 月完成博士学位。她的最终目标是加入学术机构，成为一名训练有素的科学家和富有成效的研究人员，专门从事综合、转化研究。培训环境将指导候选人，同时教授开始独立研究生涯所需的宝贵研究技术。大部分监督将由马克·D·马克尔 (Mark D. Markel) 提供，他是热组织改造领域国际知名且受人尊敬的研究者、外科教授、医学科学教授兼主席。咨询委员会将由大学内备受尊敬且经验丰富的人士组成。比较骨科研究实验室是一个完善的实验室，拥有多学科研究项目。威斯康星大学为与各个领域的专家互动提供了优越的环境，并以其卓越的研究项目而闻名，尤其是在医学科学领域。