Live cell, high speed and resolution, spectral confocal microscope

活细胞、高速、高分辨率、光谱共焦显微镜

• 批准号: 7838644
• 负责人: PETER JS SMITH
• 金额: $ 153.58万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-06 至 2011-05-05
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7838644
• 关键词: Award; Biological; Categories; Cells; Computer software; Funding; Grant; Housing; Image; Individual; Institutes; Institution; Laboratories; Lasers; Life; Marines; Microscope; Microscopy; Noise; Occupations; Postdoctoral Fellow; Principal Investigator; Recording of previous events; Research; Research Personnel; Research Project Grants; Resolution; Signal Transduction; Speed; System; Training; United States National Institutes of Health; Visit; Wood material; cellular imaging; design; detector; improved; instrument; instrumentation; member; programs

DESCRIPTION (provided by applicant): This application, from the Marine Biological Laboratory (MBL), Woods Hole, MA, requests funding for the purchase of a high end confocal microscope. We present our case for the Zeiss LSM-710 NLO/LSM Live DUO. The proposed instrument will be equipped with an array of accessories dictated by the needs of our user group - in particular our major users. The LSM-710 will replace a Zeiss LSM-510 in the Central Microscopy Facility (CMF) that was purchased in 2002. The new system takes advantage of recent advancements in lasers, detectors and software components. Spectral imaging, for example, is accomplished at a much faster rate than the LSM-510. Sensitivity and signal-to-noise are also improved. The 710 is, in short, more efficient in many ways. We further propose that the 710 has significant advantages over competing instruments and discuss these in detail. However, recognizing that this is a rapidly changing field we would reappraise the situation for significant changes if awarded this grant. The majority of our proposed research focuses on live cell imaging and cell dynamics. The submitted research projects are subdivided into four groups. The first comprises NIH funded resident and visiting re- search and include our 5 major user projects. Four of these major projects are supported by researchers' holding a total of nine relevant individual investigator NIH grants (1 Merit Award and 8 R01s). Resident research includes applications from two of the institutes within the Woods Hole Consortium; the Marine Biological Laboratory (MBL) and the Woods Hole Oceanographic Institution (WHOI). Both undertake a broad range of re- search, with strong biomedical components. Visiting research in this first category covers MBL Whitman investigators and adjunct members of the MBL Cellular Dynamics Program. The Principal Investigator directs the latter. We include 11 projects in this first category. Our second user category includes applications from investigators funded by other federal agencies (4 projects: NSF and ONR). Our third category includes visiting investigators with non-federal funding (2 projects). Lastly, in category 4, we include short descriptions for 5 relevant MBL courses, offered at the post-doctoral to professorial level, which would make use of the new instrument. Two of these are also supported by NIH grants. The Zeiss 710 would be housed in the CMF, which has an exemplary history of maintaining high end instrumentation, making it available to a broad spectrum of users, and training researchers in the instrument's optimal use. There is a long history of support from commercial entities installing instrumentation at the MBL, notably for this application from Carl Zeiss Inc. The efficiency of the newer design yields several environmental advantages and a number of jobs are intimately connected with the purchase of this high end instrument. These are discussed in detail.

描述（由申请人提供）：此申请，来自马萨诸塞州伍兹霍尔的海洋生物实验室（MBL），请求购买高端共聚焦显微镜的资金。我们介绍了Zeiss LSM-710 NLO/LSM Live Duo的案例。所提出的仪器将配备由用户组的需求（尤其是我们的主要用户）规定的一系列配件。 LSM-710将取代2002年购买的中央显微镜设施（CMF）中的Zeiss LSM-510。新系统利用了激光，探测器和软件组件的最新进步。例如，光谱成像的速度比LSM-510快得多。灵敏度和信噪比也得到改善。简而言之，710在许多方面都更有效。我们进一步建议，710比竞争工具具有显着优势，并详细讨论了这些工具。但是，认识到这是一个快速变化的领域，如果授予这笔赠款，我们将重新评估局势的重大变化。 我们提出的大多数研究都集中于活细胞成像和细胞动力学。提交的研究项目被细分为四组。第一个由NIH资助的居民和访问搜索组成，并包括我们的5个主要用户项目。这些主要项目中有四个得到了研究人员的支持，共有9名相关的个人研究员NIH赠款（1项优异奖和8个R01）。居民研究包括来自伍兹洞财团内两个机构的应用；海洋生物学 实验室（MBL）和伍兹孔海洋学机构（WHOI）。两者都有广泛的搜索，具有强大的生物医学组成部分。第一类的访问研究涵盖了MBL惠特曼研究者和MBL蜂窝动力学计划的辅助人员。主要研究人员指挥后者。我们在这一类别中包括11个项目。我们的第二个用户类别包括来自其他联邦机构资助的调查人员的应用程序（4个项目：NSF和ONR）。我们的第三类包括具有非联邦资金的访问调查员（2个项目）。最后，在第4类中，我们在博士后至教授水平上提供了5个相关MBL课程的简短描述，这些课程将利用新工具。其中两个也得到了NIH赠款的支持。 Zeiss 710将安置在CMF中，该CMF具有维持高端仪器的典范历史，可供广泛的用户使用，并培训研究人员以进行最佳使用。在MBL上安装工具的商业实体的支持很长，尤其是对于Carl Zeiss Inc.的申请。新设计的效率带来了一些环境优势，并且与购买此高端仪器的购买密切相关。这些将详细讨论。