DEVELOPING RAPID RESPONSE ION SELECTIVE ELECTRODES

开发快速响应离子选择电极

• 批准号: 7953840
• 负责人: PETER JS SMITH
• 金额: $ 3.36万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953840
• 关键词: Cells; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Data; Diffusion; Environment; Extracellular Space; Funding; Grant; Institution; Ion Channel; Ion-Selective Electrodes; Ions; Noise; Process; Reaction Time; Research; Research Personnel; Resources; Signal Transduction; Source; Testing; Time; United States National Institutes of Health; density; extracellular; improved; response; three-dimensional modeling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a subproposal within one of the core aims of the Biocurrents Research Center. We are currently developing ion-selective electrodes (ISEs) with improved response time (<1ms) and signal-to-noise ratio in order to extract meaningful single ion channel characteristics from global extracellular ionic gradients. These characteristics include mean open time, conductance and channel density. Prior to collecting cellular data and testing the above hypothesis, it is necessary to determine the influence of diffusion processes on ion fluxes at a finite distance from the point of efflux or influx. Therefore, we have set out to model the 3D environment of the boundary layer of the cell and demonstrate whether the ISE can reliably resolve unitary ion currents from clusters of ion channels within the extracellular space.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 这是生物电流研究中心核心目标之一内的一项子提案。我们目前正在开发具有改进的响应时间（<1ms）和信噪比的离子选择电极（ISE），以便从全局细胞外离子梯度中提取有意义的单离子通道特征。这些特性包括平均开放时间、电导和通道密度。在收集细胞数据并测试上述假设之前，有必要确定扩散过程对距流出或流入点有限距离处的离子通量的影响。因此，我们着手对细胞边界层的 3D 环境进行建模，并证明 ISE 是否能够可靠地解析来自细胞外空间内离子通道簇的单一离子电流。