DEVELOPING RAPID RESPONSE ION SELECTIVE ELECTRODES

开发快速响应离子选择电极

• 批准号: 7953840
• 负责人: PETER JS SMITH
• 金额: $ 3.36万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953840
• 关键词: Cells; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Data; Diffusion; Environment; Extracellular Space; Funding; Grant; Institution; Ion Channel; Ion-Selective Electrodes; Ions; Noise; Process; Reaction Time; Research; Research Personnel; Resources; Signal Transduction; Source; Testing; Time; United States National Institutes of Health; density; extracellular; improved; response; three-dimensional modeling

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This is a subproposal within one of the core aims of the Biocurrents Research Center. We are currently developing ion-selective electrodes (ISEs) with improved response time (<1ms) and signal-to-noise ratio in order to extract meaningful single ion channel characteristics from global extracellular ionic gradients. These characteristics include mean open time, conductance and channel density. Prior to collecting cellular data and testing the above hypothesis, it is necessary to determine the influence of diffusion processes on ion fluxes at a finite distance from the point of efflux or influx. Therefore, we have set out to model the 3D environment of the boundary layer of the cell and demonstrate whether the ISE can reliably resolve unitary ion currents from clusters of ion channels within the extracellular space.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这是Biocurrents研究中心的核心目标之一。我们目前正在开发具有改进的响应时间（<1ms）和信噪比的离子选择电极（ISE），以便从全局细胞外离子离子梯度中提取有意义的单个离子通道特性。这些特征包括平均开放时间，电导和通道密度。在收集细胞数据并检验上述假设之前，有必要确定扩散过程对从外排或流入点有限距离的离子通量的影响。因此，我们已经着手对单元边界层的3D环境进行建模，并证明ISE是否可以可靠地从细胞外空间内的离子通道簇中可靠地解析统一离子电流。