HYBRID SENSORS & INTEGRATED PLATFORMS

混合传感器

• 批准号: 7953829
• 负责人: PETER JS SMITH
• 金额: $ 5.6万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-12-01 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953829
• 关键词: Argon; Calcium; Cells; Computer Retrieval of Information on Scientific Projects Database; Coupling; Data Collection; Detection; Diffusion; Ensure; Epithelial; Fiber; Fiber Optics; Fluorescein; Fluoresceins; Frequencies; Funding; Grant; Hybrids; Institution; Ion-Selective Electrodes; Ionophores; Ions; Lasers; Light; Measures; Microelectrodes; Microscope; Molecular; Optics; Protons; Reporter; Research; Research Personnel; Resolution; Resources; Role; Source; Techniques; Technology; United States National Institutes of Health; base; detector; light emission; new technology; optical fiber; patch clamp; prototype; sensor; voltage

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As part of the BRC's role as an integrated technology resource, we strive to bring together different experimental approaches, providing parallel data collection from single cells. This effort encompasses hybrid sensors, single microelectrodes combining electrochemical and optic approaches, as well as the construction of integrated platforms. Hybrid Sensors Ion-selective electrodes used in the self-referencing configuration (SERIS probes) are highly sensitive, noninvasive detectors of slow diffusion gradients of ions such as protons or calcium from epithelial sheets or even from single cells. The molecular mechanisms of secretion, however, are usually controlled from within the cell, a domain into which a conventional ion-selective electrode cannot penetrate. To gain simultaneous information about the amount of ion secretion and the mechanisms controlling secretion, a dual probe is under construction. The dual probe consists of an ion-selective electrode with a fiber-optic light carrier inserted through the tip, for delivery of excitation light to an arbitrary reporter molecule. A prototype probe has been constructed, following resolution of several technical issues. To avoid destroying the selectivity of the ionophore, the optical fiber had to be silanized. After silanization, selectivity of the ionophore was unchanged, as measured with both calcium- and proton-selective ionophores. Coupling of excitation light into the optical fiber required vibrating the fiber loading end at high (20 kHz) frequencies, to ensure even delivery of optical modes and reduce laser speckle. The microscope was equipped with fluorescein-based filters to allow delivery of blue laser light (argon ion laser) and detection of green emission light. Integrated Platforms New technologies are more generally accepted when they are combined with existing techniques operating in parallel and provide mutual corroboration. The BRC already maintains combined electrochemical and electrophysiological platforms, the later encompassing single microelectrode, voltage, current and patch clamp.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 作为BRC作为集成技术资源的作用的一部分，我们努力将不同的实验方法汇总在一起，从而提供了单个单元格的并行数据收集。 这项工作涵盖了混合传感器，结合电化学和光学方法的单个微电极以及集成平台的构建。 混合传感器 自引用构型（血清探针）中使用的离子选择性电极是高度敏感的，无创的探测器，这些检测器的慢速扩散梯度（例如质子或来自单个细胞的钙）的缓慢扩散梯度（例如质子或钙）。 但是，分泌的分子机制通常是由细胞内部控制的，该域是传统的离子选择电极无法穿透的结构域。 为了获得有关离子分泌量和控制分泌的机制的同时信息，正在建造双重探针。 双重探针由一个离子选择电极组成，该电极具有插入尖端的纤维光载体，以将激励光传递到任意报告基因分子中。 在解决了几个技术问题之后，已经构建了原型探针。 为避免破坏离子载体的选择性，必须将光纤硅烷化化。 硅烷化后，通过钙和质子选择性离子载体测量的离子载体的选择性不变。 将激发光耦合到光纤中需要振动纤维载荷端在高（20 kHz）频率下，以确保什至传递光学模式并减少激光斑点。 显微镜配备了基于荧光素的过滤器，以允许递送蓝色激光（氩离子激光）并检测绿色发射光。 集成平台 当新技术与并行运行并提供相互佐证的现有技术相结合时，它们就会被普遍接受。 BRC已经维持了组合的电化学和电生理平台，后来涵盖了单个微电极，电压，电流和斑块夹。