STRUCTURAL STUDIES OF AMINOACYL-TRNA SYNTHETASES

氨酰基-TRNA 合成酶的结构研究

• 批准号: 7597981
• 负责人: JOHN J. PERONA
• 金额: $ 0.91万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7597981
• 关键词: Address; Amino Acids; Amino Acyl-tRNA Synthetases; Catalysis; Characteristics; Complex; Computer Retrieval of Information on Scientific Projects Database; Cysteine-tRNA ligase; Enzymes; Escherichia coli; Funding; Genetic Code; Glutamine-tRNA ligase; Grant; Institution; Ligands; Ligase; Pathway interactions; Protein Engineering; Research; Research Personnel; Resources; Roentgen Rays; Role; Source; Specificity; Transfer RNA; United States National Institutes of Health; Work; base; novel; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The proposed experiments investigate the structural basis of enzyme-RNA recognition and catalysis. Experiments will encompass Xray crystallographic studies of novel liganded states and of modified complexes for both the glutaminyl-tRNA synthetase and cysteinyl-tRNA synthetase complexes from E. coli. Emphasis will be placed on elucidating the stereochemical pathways of cleavage and on Xray studies that will support parallel protein engineering work to alter amino acid specificity, thus offering the potential to expand the genetic code to include incorporation of nonstandard amino acids. Other experiments will address the complex roles of indirect readout and induced-fit conformational change in conferring the very high selectivities for transfer RNA and for amino acid that are characteristic of tRNA synthetases.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 所提出的实验研究了酶-RNA 识别和催化的结构基础。 实验将包括对来自大肠杆菌的谷氨酰胺酰-tRNA 合成酶和半胱氨酰-tRNA 合成酶复合物的新型配体状态和修饰复合物进行X射线晶体学研究。 重点将放在阐明切割的立体化学途径和 X 射线研究上，这些研究将支持平行蛋白质工程工作以改变氨基酸特异性，从而提供扩展遗传密码以包括非标准氨基酸掺入的潜力。 其他实验将解决间接读出和诱导拟合构象变化的复杂作用，从而赋予转移 RNA 和 tRNA 合成酶特征的氨基酸非常高的选择性。