Acquisition of Xray Diffraction Instrumentation

购置 X 射线衍射仪器

• 批准号: 7219644
• 负责人: JOHN J. PERONA
• 金额: $ 47.91万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7219644
• 关键词: Advisory Committees; Animal Model; Anodes; Area; Arts; Biochemistry; Biocompatible Materials; Caenorhabditis elegans Proteins; Cells; Charge; Chemistry; Collagen; Complex; Copper; Development; Developmental Biology; Devices; Engineering; Environment; Floor; Frequencies; Funding; Gene Expression Regulation; Growth; Health; Housing; Image; Incubators; Laboratories; Laboratory Research; Maintenance; Mediating; Medical; Microprocessor; Molecular; Molecular Structure; Nucleic Acids; Numbers; Optics; Personal Satisfaction; Process; Proteins; Research; Research Personnel; Resolution; Resource Sharing; Roentgen Rays; Science; Signal Transduction; System; Therapeutic; Therapeutic Intervention; X-Ray Diffraction; base; cost; detector; foot; instrument; instrumentation; microbial disease; novel; programs; research study; response; tool; tumorigenesis

DESCRIPTION (provided by applicant): A Bruker X-ray high-intensity instrumentation system is requested to enable single crystal highresolution Xray diffraction experiments for the determination of the molecular structures of proteins, nucleic acids, and higher-order assemblies. The system combines a high frequency microprocessor-controlled microfocus rotating anode X-ray generator, high-brilliance multilayer optics providing very high monochromaticity, and a 4K charged-couple device (CCD) area detector with high resolution phosphor suitable for copper wavelengths. This instrumentation will provide NIH-funded investigators with a state-of-the-art system capable of delivering the highest intensity available in an in-house Xray instrument. The new system will provide a powerful tool for structural studies to enable the research of PI's in the Chemistry and Biochemistry Department, Molecular, Cellular and Developmental Biology Department, and Materials Research Laboratory at UCSB. The instrument will be housed in a 2000 square-foot laboratory located on the fourth floor of the Chemistry and Biochemistry Department, and will replace an existing Rigaku image-plate system that was purchased in 1994 and is well behind existing state-of-the-art in the field. This laboratory includes incubators for crystal growth and laboratory space for optimal examination and manipulation of crystals prior to the diffraction experiment, and serves as a further shared resource. The investigators will utilize the instrument to conduct research in broad areas of basic medical science, including structural studies of protein-nucleic acid complexes crucial to gene regulation, of unusual collagens with important biomaterials engineering applications, of proteins central to the unfolding of the developmental program in the model organism C. elegans, and of proteins involved in two-component signaling networks mediating the responses of cells to their environments. The instrument will be managed by a staff researcher as a shared facility, under the direction of an internal advisory committee composed of a number of the PI's listed in this proposal. A recharge system will be set up to generate the funds used to cover maintenance costs. Statement of Relevance: The acquisition of a rotating anode generator and state-of-the-art CCD detector will provide a crucial tool to enable the research of a large number of NIH-funded investigators at UCSB. These projects have fundamental health relevance including the development of novel protein-based therapeutics and potential therapeutic interventions into microbial diseases and the process of oncogenesis.

描述（由申请人提供）：需要布鲁克 X 射线高强度仪器系统来进行单晶高分辨率 X 射线衍射实验，以确定蛋白质、核酸和高阶组装体的分子结构。该系统结合了高频微处理器控制的微焦点旋转阳极 X 射线发生器、提供极高单色性的高亮度多层光学器件以及具有适用于铜波长的高分辨率磷光体的 4K 电荷耦合器件 (CCD) 区域探测器。该仪器将为 NIH 资助的研究人员提供最先进的系统，能够提供内部 X 射线仪器中可用的最高强度。新系统将为结构研究提供强大的工具，使 UCSB 化学与生物化学系、分子、细胞和发育生物学系以及材料研究实验室的 PI 能够进行研究。该仪器将安装在化学与生物化学系四楼 2000 平方英尺的实验室中，并将取代现有的 Rigaku 图像板系统，该系统于 1994 年购买，远远落后于现有的最先进技术。领域的艺术。该实验室包括用于晶体生长的培养箱和用于在衍射实验之前对晶体进行最佳检查和操作的实验室空间，并作为进一步的共享资源。研究人员将利用该仪器在基础医学的广泛领域进行研究，包括对基因调控至关重要的蛋白质-核酸复合物的结构研究、具有重要生物材料工程应用的不寻常胶原蛋白、对发育程序的展开至关重要的蛋白质的结构研究在模式生物秀丽隐杆线虫中，以及参与介导细胞对其环境反应的双组分信号网络的蛋白质。该仪器将由一名研究人员作为共享设施进行管理，并在由本提案中列出的一些 PI 组成的内部咨询委员会的指导下进行管理。将建立一个充值系统来产生用于支付维护费用的资金。相关性声明：购买旋转阳极发生器和最先进的 CCD 探测器将为 UCSB 的大量 NIH 资助的研究人员的研究提供重要的工具。这些项目具有基本的健康相关性，包括开发基于蛋白质的新型疗法以及对微生物疾病和肿瘤发生过程的潜在治疗干预措施。