Sacrificial templated grafts to encourage bone healing through mechanotransduction

牺牲模板移植物通过机械传导促进骨愈合

基本信息

批准号：
10811305
负责人：
JOO L. ONG
金额：
$ 41.17万
依托单位：
UNIVERSITY OF TEXAS SAN ANTONIO
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-18 至 2025-09-17
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10811305
关键词：
3D Print Affect Anatomy Autologous Biocompatible Materials Biological Biomechanics Bioreactors Blood Vessels Bolus Infusion Bone Regeneration Bone Tissue Bone Transplantation Bone callus Bone plates Breathing Calcium Cancerous Cell Density Cells Chemotactic Factors Clinical Collagen Congenital Abnormality Coupled Coupling Data Defect Development Disease Distraction Osteogenesis Engineering Ensure Environment Esthetics Excision Exhibits Face Film Goals Growth Factor Head Health Care Costs Hematopoietic stem cells Implantation procedure In Situ In Vitro Individual Injury Intervention Jaw Liquid substance Maintenance Mandible Mastication Mechanical Stimulation Mechanics Mesenchymal Stem Cells Modeling Morbidity - disease rate Natural regeneration Neck Operative Surgical Procedures Osteogenesis Pain Pathway interactions Patients Perfusion Periodicity Physiological Polymers Porosity Printing Process Psychological Stress Quality of life Rattus Research Savings Shapes Signal Transduction Site Skeleton Speech Surface Surgeon System Techniques Thick Time Tooth Extraction Traumatic injury Vascularization Work angiogenesis bone bone fracture repair bone healing bone loss calcium phosphate cancer therapy cell motility craniofacial craniofacial bone craniomaxillofacial design efficacy study functional restoration healing improved in vivo innovation instrumentation mechanical load mechanical properties mechanotransduction meetings novel osteogenic particle public health relevance recruit regenerative response restoration sample fixation scaffold substantia spongiosa

项目摘要

Sacrificial Templated Grafts to Encourage Bone Healing Through Mechanotransduction Project Summary Loss of large volumes of bone tissue often occurs as a result of traumatic injuries or cancerous resection. Such injuries, especially in the head, jaws, and neck are challenging to surgeons and the long- and short-term complications as well as the quality of life for these patients are often affected due to alteration in facial aesthetics and features, speech, mastication, and breathing. To overcome these complications, current clinical alternatives include grafting of bone with associated vascular bundles obtained from a secondary site. All these treatments require multiple clinical interventions and often result in increased pain or donor site morbidity for the patients. In this study, single-surgical step synthetic graft alternatives with controlled mechano-modulation through compliance changes within the scaffold as well as a concerted delivery of cell migration factors to enhance bone regeneration will be developed. As such, the 2 aims for this project are 1) to develop a chemoattractant incorporated bone graft substitute with engineered degradation zones while retaining its mechanical integrity for functional stabilization, and 2) to assess controlled temporal degradation under physiologically relevant conditions in vitro and mechanisms of osteogenic and angiogenic signaling through mechanotransduction and Ca2+ coupled pathways. Data collected from the in vitro and in vivo studies will guide the development of this novel graft and provide an established platform to study the effects of local micromechanical factors and release of recruitment factors to promote fracture healing and bone regeneration without need for extensive bone fixation instrumentation.

牺牲模板的移植物通过机械转移来鼓励骨骼愈合项目摘要大量骨组织的损失通常是由于创伤性损伤或癌性切除而导致的。这样的伤害，尤其是头部，下巴和颈部，对外科医生和短期和短期都充满挑战由于面部美学的改变，这些患者的并发症以及这些患者的生活质量通常受到影响以及功能，语音，咀嚼和呼吸。为了克服这些并发症，当前的临床替代方案包括将骨骼与从次级部位获得的相关血管束的接枝。所有这些治疗方法需要多种临床干预措施，通常会导致患者的疼痛或供体部位发病。在这项研究中，单手术步骤合成移植物具有通过脚手架内的合规性变化以及细胞迁移因子的一致传递以增强骨骼将开发再生。因此，该项目的两个目标是1）开发趋化剂与工程降解区合并的骨移植替代品，同时保留其机械完整性功能稳定和2）评估生理上相关的受控时间降解通过机械转导的成分和血管生成信号传导的体外条件和机制 Ca2+耦合途径。从体外和体内研究收集的数据将指导这一发展新颖的移植物，并提供了研究局部微力因素的影响并释放的既定平台招募因素以促进骨折愈合和骨骼再生，而无需广泛的骨骼固定仪器。