EARLY BONE FORMATION AT BONE BIOMATERIAL INTERFACE

骨生物材料界面的早期骨形成

• 批准号: 6863766
• 负责人: JOO L. ONG
• 金额: $ 14.75万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-02-01 至 2006-08-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6863766
• 关键词: X ray crystallography; biomaterial interface interaction; biomaterials; calcium ion; cell differentiation; cell proliferation; dental implants; fibronectins; flow cytometry; hydroxyapatites; infrared spectrometry; interferometry; laboratory rabbit; laboratory rat; medical implant science; nucleic acid probes; oligonucleotides; osteoblasts; osteogenesis; osteopontin; phosphates; polymerase chain reaction; titanium

DESCRIPTION (Verbatim from the Applicant): The goal of this research is to gain a better understanding of the biological basis for successful orthopaedic and dental implant therapy by elucidating the early phenomena that govern osseointegration. In this proposal, the effect of sputtered hydroxyapatite (HA) crystallinity on early bone cell activity in vitro and in vivo will be investigated under highly controlled and defined conditions. Our overall hypothesis is that, under conditions where other variables are controlled, the degree of crystallinity of the HA surface directly affects early bone cell activity in vitro and the rate of development of osseointegration in vivo. The objective of this study is to correlate the effect of characterized HA crystallinity to dissolution and protein adsorption, bone cell response in vitro and early cell activities in vivo. In this proposal, Aim 1 will be to determine the relationship between crystalline content of well-characterized HA surfaces and 1) the adsorption of specific extracellular matrix proteins, fibronectin and osteopontin, and 2) the rate of dissolution of the surface. The HA and Ti coatings will be produced using sputter coating. The rationale for using the sputtering technology is due to the high coating-metal adhesion strength compared to plasma spraying. Protein adsorption and dissolution of the coatings will be measured over time. Aim 2 will determine the extent to which the crystalline content of HA surfaces effects osteoblast proliferation, differentiation, and metabolism in vitro. It is hypothesized in this aim that because osteoblast proliferation and differentiation may be affected by either the adsorption of specific extracellular matrix proteins, fibronectin and osteopontin, or the rate of dissolution of the surface, or both; metabolic activity leading to mineral formation will vary with the crystalline content of the HA surface. Implicit in this hypothesis is there exists an optimal crystalline content of an HA surface for the promotion of bone formation activity. Aim 3 will evaluate the extent to which the crystalline content of HA surfaces affects osseointegration in vivo. Early bone activity will be evaluated using histology, mechanical strength and immunohistochemistry in this aim. Data generated from this study will provide information on the early maturation of bone cells in the presence of implant biomaterials and will provide a correlation between biomaterial properties and bone cell responses in vitro and in vivo. Additionally, information generated will contribute to the development of an ideal implant surface, thereby reducing long-term implant failures.

描述（逐字病申请人）：这项研究的目的是获得 更好地理解成功骨科的生物学基础 牙科植入疗法通过阐明治理的早期现象 骨整合。在此提案中，溅射羟基磷灰石的作用（HA） 体外和体内早期骨细胞活性的结晶度将是 在高度控制和定义的条件下进行了调查。我们的整体 假设是，在控制其他变量的条件下 HA表面的结晶度直接影响早期骨细胞 体外活性和体内骨整合的发育速率。这 这项研究的目的是关联特征HA的影响 对溶解和蛋白质吸附的结晶度，骨细胞反应 体内的体外和早期细胞活性。在此提案中，目标1将是 确定特征良好的HA的结晶含量之间的关系 表面和1）特定细胞外基质蛋白的吸附， 纤连蛋白和骨桥蛋白，以及2）表面溶解速率。这 HA和TI涂层将使用溅射涂层生产。理由 使用溅射技术是由于高涂层金属粘附 与血浆喷涂相比强度。蛋白质吸附和溶解 涂料将随着时间的推移进行测量。 AIM 2将确定多大程度的程度 HA表面的结晶含量会影响成骨细胞增殖， 分化和代谢体外。在这个目标中假设 因为成骨细胞增殖和分化可能会受到任何一个 特定细胞外基质蛋白，纤连蛋白和 骨桥蛋白，或表面溶解速率，或两者兼而有之；代谢 导致矿物形成的活动将随着晶体含量而变化 HA表面。在这个假设中隐含的是最佳的 HA表面的结晶含量以促进骨形成 活动。 AIM 3将评估HA的结晶含量的程度 表面会影响体内骨整合。早期的骨骼活动将是 使用组织学，机械强度和免疫组织化学评估 目的。这项研究产生的数据将提供有关早期的信息 在植入物生物材料存在下骨细胞的成熟，将 在生物材料特性与骨细胞反应之间提供相关性 体内和体内。此外，生成的信息将有助于 开发理想的植入物表面，从而减少长期植入物 失败。