Core 1: Tumor Processing and Single Cell RNA sequencing Core

核心1：肿瘤处理和单细胞RNA测序核心

基本信息

批准号：
10684050
负责人：
Kai W Wucherpfennig
金额：
$ 34.98万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-03 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10684050
关键词：
Animal Model Blood Brain Neoplasms Cell Separation Cells Clinical Clinical Trials Clonal Expansion Combination immunotherapy Complement Complex DNA Sequencing Facility Data Data Set Diagnosis Ecosystem Ensure Epigenetic Process Equipment Experimental Models Flow Cytometry Gene Expression Genetic Genetic Transcription Genomics Genotype Glioblastoma Goals Heterogeneity Human Human Resources Immune Immunologics Immunosuppression Immunotherapy Individual Knowledge Length Malignant Neoplasms Methods Molecular Immunology Myeloid Cells Operative Surgical Procedures Pathway interactions Patients Peptide Vaccines Phase Population Premalignant Cell Quality Control Reagent Recurrence Recurrent tumor Regulatory Pathway Relapse Reproducibility Research Personnel Resolution Resources Sampling Services Specimen Standardization T cell infiltration T-Lymphocyte Techniques Technology Testing Transcript anti-PD1 antibodies antigen-specific T cells cancer cell cancer infiltrating T cells conventional therapy data sharing genetic information mouse model neoantigen vaccine neoantigens novel therapeutic intervention participant enrollment programs single cell technology single-cell RNA sequencing success technology platform tool transcriptome tumor

项目摘要

Abstract Single cell RNA-sequencing (scRNA-seq) has emerged as a powerful approach to dissect tumors and their ecosystems by determining the state of individual cells and inferring partial genetic information from expressed transcripts. For infiltrating T cells, this provides a comprehensive approach to define T cell states in human tumors. For malignant cells, scRNA-seq paves the way to characterizing cellular states and their associated genotypes at cellular resolution. The Molecular Immunology Core (Core 1) provides standardized and reproducible methods to profile and characterize immune cells and malignant cells by scRNA-seq in glioblastoma in both patients and mouse models. Core 1 also provides expert personnel, facilitates the sharing of knowledge within the program, and maintain the sophisticated equipment necessary to generate data for all Projects. The Molecular Immunology Core will be led Dr. Mario Suvà and Dr. Kai Wucherpfennig who will contribute their expertise in single-cell technologies and immune cell isolation.

抽象的单细胞 RNA 测序 (scRNA-seq) 已成为解剖肿瘤和癌症的有效方法。通过确定单个细胞的状态并推断部分遗传信息来了解其生态系统对于浸润性 T 细胞，这提供了一种定义 T 的综合方法。对于人类肿瘤中的细胞状态，scRNA-seq 为表征细胞铺平了道路。细胞分辨率下的状态及其相关基因型。提供标准化和可重复的方法来分析和表征免疫细胞还通过 scRNA-seq 对患者和小鼠模型中的胶质母细胞瘤中的恶性细胞进行了研究。提供专家人员，促进项目内的知识共享，并维持为所有项目生成数据所需的先进设备。由 Mario Suvà 博士和 Kai Wucherpfennig 博士领导，他们将贡献他们在单细胞方面的专业知识技术和免疫细胞分离。