ShEEP Request for A Namocell PALA Single CellDispenser

ShEEP 请求 Namocell PALA 单细胞分配器

• 批准号: 10739131
• 负责人: Joseph Anthony Garcia
• 金额: --
• 依托单位: JAMES J PETERS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10739131
• 关键词: Acceleration; Address; Aging; Alzheimer' s Disease; Anemia due to Chronic Disorder; Animal Disease Models; Animal Experiments; Animal Model; Antibodies; Arkansas; Biochemical; Biomedical Research; Blood; CRISPR/Cas technology; Cell Culture Techniques; Cell Line; Cell Separation; Cell Survival; Cell model; Cells; Clone Cells; Cloning; Clustered Regularly Interspaced Short Palindromic Repeats; Contract Services; DNA; Data; Diabetes Mellitus; Disease; Engineering; Equipment; Evaluation; Flow Cytometry; Funding Opportunities; Generations; Genetic; Genetic Engineering; Genetic study; Genomic Library; Glean; Goals; Health; Human; Human Genetics; Industry; Infectious Agent; Kidney; Kidney Diseases; Lasers; Liquid substance; Malignant Neoplasms; Medical; Metabolic; Metabolic Diseases; Microfluidics; Mind; Modeling; Modernization; Modification; Molecular; Mus; N phosphonoacetyl L aspartate; National Institute of Drug Abuse; Nature; Neurodegenerative Disorders; Neurologic; Noise; Organ; Patients; Pharmaceutical Preparations; Population; Proteins; RNA; Recovery; Reproducibility; Research; Research Personnel; Rodent; Rodent Model; Sheep; Shipping; Signal Transduction; Sorting; Spinal cord injury; Structure; Study models; System; Techniques; Therapeutic; Tissues; Training; Transfection; Transformed Cell Line; Traumatic Brain Injury; United States National Institutes of Health; Update; Variant; Veterans; Virus; applied biomedical research; cell transformation; cell type; diet-induced obesity; human disease; human model; improved; induced pluripotent stem cell; innovation; insight; instrument; interest; microfluidic technology; miniaturize; multidisciplinary; multiple omics; novel; operation; precision medicine; preservation; pressure; programs; rapid test; single cell analysis; spinal cord and brain injury; synthetic biology

We are requesting a Namocell PALA Single Cell Dispenser, a benchtop instrument that combines innovative microfluidics technology with flow cytometry and liquid dispensing. It can sort and dispense single cells into 96 or 384-well plates in minutes. Its low-pressure operation (<2 psi) preserves cell viability as well as integrity. The ability to isolate specific cell populations is invaluable for modern basic and applied biomedical research. Microfluidic droplet dispensers provide for miniaturized, high-throughput isolation of single cells in a microenvironment, which can be leveraged for ultra-sensitive and rapid assays. These units are ideal for a wide range of single cell applications including cell cloning with improved outgrowth, generation of high-yield single cell genomics libraries, rapid bulk sorting of specific target cells or rare cell populations, and recovery of single cells from as few as 100 cells. Single cell dispensers are currently in use in several academic and industry settings. Recent acquisition of Namocell Single Cell Dispensers by federally supported facilities include NIH/NIDA and Arkansas Little Rock VA. Data obtained with the PALA Single Cell Dispenser will have applications for both cell culture as well as rodent model studies of human disease. As such, we expect it will aid us in deciphering how these diseases develop or progress as well as help in identifying new treatment opportunities for Veterans with these diseases. The expected research applications for the Namocell PALA Single Cell Dispenser at JJP VAMC will range from bulk sorting of homogenous cell populations to single cell isolation. Bulk sorting is useful for initial interrogations of highly enriched, specific cell types. We expect JJP VAMC investigators who use rodent models of human diseases including those with neurological, renal, metabolic, or cancer relevance will use the PALA for bulk sorting of specific or rare cell populations, particularly those residing in organs with heterogenous cell types [Major Users: JG, CC, GP, RR]. Bulk sorting is also useful for identifying cells manipulated in culture that are marked by fluorescent activity, such as transfected cells expressing specific fluorescent proteins (FP). The dual laser system in the PALA will allow for selection of cells expressing dual exogenous proteins with distinct FP surrogates without the need for prolonged drug selection, which otherwise can introduce confounding factors [Major Users: JG, CC, RR]. The Namocell PALA Single Cell Dispenser will also be used for purification of single cells. Single-cell analysis can discriminate signal from noise in a heterogeneous cell population and thereby facilitate multiomic studies of rare cell populations obtained from tissues or blood of healthy or disease animal models [Major Users: JG, CC, GP, RR]. The PALA will also facilitate high-throughput isolation of single cells in a defined microenvironment, which can be leveraged for ultra-sensitive and rapid assays [Major Users: JG, GP, RR]. Finally, we anticipate great interest in the use of the PALA for cloning of precision genetically engineered transformed cells or induced pluripotent stem cells (iPSC), which may offer insights into structure-function studies as well as human disease contributions from nonsynonymous variants identified in human genetic studies, respectively [Major Users: JG, CC, GP, RR].

我们要求使用Namocell Pala单细胞分配器，这是一种台式仪器 将创新的微流体技术与流式细胞仪和液体分配结合在一起。它可以 在几分钟内将单个细胞和分配单元分为96或384孔板。其低压操作（<2 PSI）保留细胞活力和完整性。分离特定细胞群体的能力是 对于现代基础和应用生物医学研究而言，无价。微流体液滴分配器 提供微型细胞在微环境中的微型，高通量分离， 可以利用超敏感和快速测定。这些单元非常适合广泛 单细胞应用，包括细胞克隆，有改善的生长，高产的产生 单细胞基因组学库，特定靶细胞或稀有细胞群的快速分类， 并从少于100个细胞中恢复单个细胞。 单细胞分配器目前正在几个学术和行业环境中使用。最近的 通过联邦支持的设施收购Namocell单细胞分配器包括 NIH/NIDA和Arkansas Little Rock VA。使用帕拉单细胞分配器获得的数据将 在细胞培养物以及人类疾病的啮齿动物模型研究中都有应用。像这样， 我们希望这将有助于我们破译这些疾病如何发展或进展以及帮助 确定这些疾病的退伍军人的新治疗机会。 JJP VAMC的Namocell Pala单细胞分配器的预期研究应用 从均质细胞群体到单细胞分离的批量排序范围。批量排序是 对于高度富集的特定细胞类型的初始询问有用。我们希望JJP VAMC 使用人类疾病的啮齿动物模型的研究人员，包括神经，肾脏， 代谢或癌症相关性将使用pala进行特定或稀有细胞的批量排序 种群，特别是那些居住在具有异质细胞类型的器官中的人群[主要用户：JG， CC，GP，RR]。批量排序也可用于识别在培养中操纵的细胞，这些细胞被标记为 通过荧光活性，例如表达特定荧光蛋白（FP）的转染细胞。 pala中的双激光系统将允许选择表达双重外源的细胞 具有不同FP代理的蛋白质无需长时间的药物选择，这 否则可以引入混杂因素[主要用户：JG，CC，RR]。 Namocell Pala单细胞分配器也将用于纯化单细胞。 单细胞分析可以将信号与异质细胞群中的噪声区分开，并且 从而促进了从组织或血液中获得的罕见细胞群体的多媒体研究 健康或疾病动物模型[主要用户：JG，CC，GP，RR]。帕拉也将有助于 在定义的微环境中的单个细胞的高通量隔离，可以利用它 对于超敏感和快速测定[主要用户：JG，GP，RR]。最后，我们期待很棒 对使用Pala用于克隆精确基因转化细胞的兴趣 或诱导多能干细胞（IPSC），可以提供对结构功能研究的见解 以及人类遗传中鉴定出的非同义变体的人类疾病贡献 研究分别[主要用户：JG，CC，GP，RR]。