Flow Cytometry Shared Resource

流式细胞术共享资源

• 批准号: 10661659
• 负责人: Remi J Creusot
• 金额: $ 12.54万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-04 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10661659
• 关键词: Cancer Biology; Cancer Center Support Grant; Cell Separation; Cell surface; Cells; Color; Complex; Computer software; Computers and Advanced Instrumentation; Conceptions; Consultations; Data; Data Analyses; Dedications; Development; Doctor of Philosophy; Education; Equipment; Event; Flow Cytometry; Flow Cytometry Shared Resource; Funding; Goals; Hematopoietic; Herbert Irving Comprehensive Cancer Center; Hour; Human Resources; Immune; Immunology; Infiltration; Infrastructure; Intracellular Signaling Proteins; Journals; Lasers; Leadership; Lymphoid Cell; Maintenance; Malignant Neoplasms; Manuscripts; Metabolic Marker; Myeloid Cells; Nature; Needs Assessment; Peer Review; Population; Preparation; Protocols documentation; Publications; Qualifying; Reproducibility; Research; Research Personnel; Research Project Grants; Research Support; Sampling; Scientist; Secure; Services; Solid; Sorting; Speed; Stains; Techniques; Technology; Time; Training; Training and Education; Trees; United States National Institutes of Health; Walking; anticancer research; cancer cell; cancer immunotherapy; cancer therapy; complex data; cost effective; cost effectiveness; data acquisition; density; design; detector; empowerment; experience; high dimensionality; high standard; high throughput screening; indexing; insight; instrument; instrumentation; leukemia/lymphoma; member; multidimensional data; neoplastic cell; new technology; novel; programs; prospective; service providers; stem cells; success; technology development; technology validation; tool; transcription factor; translational immunology; tumor; tumor immunology

FLOW CYTOMETRY SHARED RESOURCE: PROJECT SUMMARY The Flow Cytometry Shared Resource (FCSR) gives access to flow cytometry analysis and cell sorting instruments and services to Herbert Irving Comprehensive Cancer Center (HICCC) members. Under the leadership of Remi Creusot, PhD, the FCSR provides: (1) multi-color panel flow data acquisition and analysis using flow cytometry analyzers, including high throughput screening (HTS), (2) flow cytometry cell sorting (four- way or six-way or plate) using high speed cell sorters by dedicated operators of FCSR, including single cell index sorting, and (3) comprehensive consultation, panel design, hands-on training, troubleshooting, and data analysis services by PhD-level FCSR scientists. This broad and inclusive package of services enables HICCC investigators to analyze tumor cell populations and their microenvironment, including the tumor-infiltrating lymphoid and myeloid cells at the single cell level. During the current project period (2014-2019), the FCSR has been integrated with the flow cytometry core of the Columbia Center for Translational Immunology (CCTI). The merging of these facilities has secured access to a broader range of state-of-the-art instrumentation and brought advanced expertise in immunology to the research of HICCC flow cytometry users. HICCC members now have access to nine instruments, including five analyzers and four sorters. In addition, two of the existing instruments/analyzers have been upgraded to 18-20 detectors. Moreover, a new Influx sorter has been acquired to expand the capacity of sorting services. Upgrades in consultation services have further empowered the research of HICCC members who now benefit from face-to-face consultation on panel design, protocol optimization, and hands-on training on data acquisition, analysis, and experimental troubleshooting essential to maximize the impact of new advanced instruments for high dimensional multi-color panels. Based on its advanced instrumentation, highly qualified staff, broad portfolio of specialized services, easy accessibility, and cost-effective services, the FCSR is the flow cytometry service provider of choice for HICCC members. The FCSR will continue to incorporate the latest technologies and to provide high quality cell sorting, analytical and consultation services to HICCC members to empower their understanding of cancer biology and to enable new breakthroughs in cancer therapy. Over the current project period, the capabilities of the FCSR have been utilized by 81 HICCC members, provided key data and insights to support the success of more than 46 HICCC member peer-reviewed publications, including 23 contributions in journals with impact factor >10, of which 12 were in journals with an impact factor >20 (Nature, Cell Stem Cell, Cancer Cell, Cancer Discovery), and currently support research for 21 NIH-funded research grants (11 from NCI).

流式细胞仪共享资源：项目摘要 流式细胞仪共享资源（FCSR）可访问流式细胞仪分析和细胞分类 赫伯特·欧文（Herbert Irving）综合癌症中心（HICCC）成员的仪器和服务。在 FCSR的Remi Creusot博士领导才提供：（1）多色面板流数据获取和分析 使用流式细胞仪分析仪，包括高吞吐量筛选（HTS），（2）流式细胞仪细胞分类（四 使用FCSR的专用操作员使用高速单元格拉术器的方式或六向或板），包括单细胞指数 分类和（3）全面咨询，面板设计，动手培训，故障排除和数据分析 博士级FCSR科学家的服务。这种广泛而包容的服务包使HICCC 研究人员分析肿瘤细胞群体及其微环境，包括肿瘤浸润 单细胞水平的淋巴样和髓样细胞。在当前项目期（2014- 2019年）中，FCSR有 与哥伦比亚转化免疫学中心（CCTI）的流式细胞仪核心集成。这 这些设施的合并已确保获得更广泛的最先进的仪器，并带来了 HICCC流式细胞仪使用者研究的免疫学高级专业知识。 HICCC成员现在有 访问九种乐器，包括五个分析仪和四个分类器。此外，两个现有 仪器/分析仪已升级到18-20个探测器。此外，已经获得了一个新的涌入分把 扩大分类服务的能力。咨询服务中的升级进一步授权 HICCC成员的研究，这些成员现在受益于面板设计的面对面咨询，协议 优化以及对数据获取，分析和实验故障排除至关重要的实践培训 最大化新的高级仪器对高维多色面板的影响。基于它的 高级仪器，高素质的员工，广泛的专业服务组合，易于访问性，以及 FCSR是具有成本效益的服务，是HICCC成员首选流式细胞仪服务提供商。这 FCSR将继续融合最新的技术，并提供高质量的细胞分类，分析和 向HICCC成员咨询服务，以增强他们对癌症生物学的理解并启用新的 癌症治疗的突破。在当前的项目期间，FCSR的功能已被利用 由81位HICCC成员提供了关键数据和见解，以支持超过46位HICCC成员的成功 经过同行评审的出版物，包括影响因子> 10的期刊中的23个捐款，其中12个在 具有影响因子> 20的期刊（自然，细胞干细胞，癌细胞，癌症发现），目前支持 对21种NIH资助的研究补助金的研究（NCI的11项）。