Flow Cytometery and Cell Sorting Core

流式细胞仪和细胞分选核心

基本信息

批准号：
10408970
负责人：
Steven A Porcelli
金额：
$ 21.52万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-05 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10408970
关键词：
Address Adopted Antibodies Area Biological Biology Budgets Cancer Biology Cancer Center Support Grant Cell Nucleus Cell Separation Cells Characteristics Complex Core Facility Development Ensure Environment Epithelial Equipment Faculty Flow Cytometry Fluorescence-Activated Cell Sorting Fluorochrome Funding Gene Expression Goals Human Resources Individual Infrastructure Institution Lead Mesenchymal Metastatic Neoplasm to the Lung Methodology Methods Neoplasm Metastasis Phenotype Population Procedures Property Reporter Research Personnel Resources Seeds Services Signal Pathway Signal Transduction Sorting - Cell Movement Speed Structure Suspensions Techniques Technology Time Tissues Update Variant Work antibody conjugate base cell preparation cell type cost effective design experimental study high dimensionality innovation instrument interest malignant breast neoplasm neoplastic cell new technology novel strategies programs protein expression ranpirnase recruit single cell analysis single cell technology stem cells stem-like cell technology validation tumor tumor microenvironment

项目摘要

Core B – Flow Cytometry and Cell Sorting Core (Core Lead: Porcelli) Program Lead: Backer, Jonathan M. ABSTRACT The Flow Cytometry Analysis and Cell Sorting Core (Core B) will provide access to equipment and expertise for a variety of advanced flow cytometry-based technologies for single cell level analysis and isolation. The core will support and maintain a range of advanced instruments for analytic and sorting applications, and will actively guide the program investigators in applying these to their studies on cancer metastasis. These methodologies will be applied for the identification, isolation and detailed profiling of cell types of interest and relevance to the goals of the program project. The resources of the core will be used extensively in all projects comprising this program, and their organization and support under this structure will provide the Program investigators with access and technical support in a cost effective way. The key personnel of the core will have effort dedicated to directly assisting the program PIs in the development and implementation of single cell analysis techniques relevant to their projects, and assist them in innovating new approaches using the core technologies. By providing an organizing nucleus for a portfolio of flow cytometry-based single cell analysis and isolation methods, Core B will create an infrastructure and environment for adapting these evolving technologies to the needs of the PPG investigators. All of the essential equipment and expertise required for this core are already in place and available at our institution. Key personnel with the necessary background and expertise have been recruited from existing faculty and staff, and these individuals have been designated appropriate levels of effort to ensure proper functioning of Core B. While some of the equipment and personnel associated with the core are physically housed and partially supported within our NCI CCSG (P30) funded Flow Cytometry Core Facility, the creation of Core B is essential to the successful accomplishment of the goals of the PPG, as it will enable the implementation of these technologies for new and innovative applications that specifically address the studies of cancer metastasis that form the theme of the current program application. PHS 398/2590 (Rev. 11/07) Page

核心B - 流式细胞术和细胞分选核心（核心铅：猪）程序铅：支持者，乔纳森·M。抽象的流式细胞仪分析和细胞排序核心（核心B）将提供对设备和专业知识的访问用于各种基于流式细胞仪的技术，用于单细胞水平分析和隔离。这核心将支持并维护一系列用于分析和分类应用程序的高级工具，并将积极指导该计划的研究人员将这些应用于其对癌症转移的研究。这些方法将用于识别，隔离和详细分析细胞类型的感兴趣和与计划项目的目标相关。核心的资源将在所有项目中广泛使用完成该计划及其在此结构下的组织和支持将提供该计划具有成本效益的访问和技术支持的调查人员。核心的关键人员将致力于直接协助PIS开发和实施单一的计划与其项目相关的细胞分析技术，并协助他们使用该技术来创新新方法核心技术。通过为基于流式细胞术的单细胞组合提供组织核心分析和隔离方法，核心B将创建一个基础架构和环境，以适应这些在PPG研究人员的需求中不断发展的技术。所有基本设备和专业知识该核心的要求已经到位，并在我们的机构提供。有必要的关键人员背景和专业知识是从现有教职员工招募的，这些人已经被设计了适当的努力，以确保核心B的正常运作。与核心相关的设备和人员在我们的内部有物理内置并部分支持 NCI CCSG（P30）资助的流式细胞仪核心设施，核心B的创建对于成功实现PPG的目标，因为这将使这些技术实施新的新技术以及专门针对构成癌症转移研究的研究的创新应用当前的程序申请。 PHS 398/2590（Rev. 11/07）页面