Flow Cytometery and Cell Sorting Core

流式细胞仪和细胞分选核心

基本信息

批准号：
10659181
负责人：
Steven A Porcelli
金额：
$ 21.09万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-07-05 至 2027-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10659181
关键词：
Address Adopted Antibodies Area Biological Biology Budgets Cancer Biology Cancer Center Support Grant Cell Nucleus Cell Separation Cells Characteristics Complex Core Facility Dedications Development Ensure Environment Epithelium Equipment Faculty Flow Cytometry Fluorescence-Activated Cell Sorting Fluorochrome Funding Gene Expression Goals Human Resources Individual Infrastructure Institution Lead Mesenchymal Metastatic Neoplasm to the Lung Methodology Methods Neoplasm Metastasis Phenotype Population Procedures Property Reporter Research Personnel Resources Services Signal Pathway Signal Transduction Sorting Speed Structure Suspensions Techniques Technology Time Tissues Update Variant Work antibody conjugate cell type cost effective design experimental study high dimensionality innovation instrument interest malignant breast neoplasm neoplastic cell new technology novel strategies programs protein expression recruit single cell analysis single cell technology stem cells stem-like cell technology platform technology validation tumor tumor microenvironment

项目摘要

Core B – Flow Cytometry and Cell Sorting Core (Core Lead: Porcelli) Program Lead: Backer, Jonathan M. ABSTRACT The Flow Cytometry Analysis and Cell Sorting Core (Core B) will provide access to equipment and expertise for a variety of advanced flow cytometry-based technologies for single cell level analysis and isolation. The core will support and maintain a range of advanced instruments for analytic and sorting applications, and will actively guide the program investigators in applying these to their studies on cancer metastasis. These methodologies will be applied for the identification, isolation and detailed profiling of cell types of interest and relevance to the goals of the program project. The resources of the core will be used extensively in all projects comprising this program, and their organization and support under this structure will provide the Program investigators with access and technical support in a cost effective way. The key personnel of the core will have effort dedicated to directly assisting the program PIs in the development and implementation of single cell analysis techniques relevant to their projects, and assist them in innovating new approaches using the core technologies. By providing an organizing nucleus for a portfolio of flow cytometry-based single cell analysis and isolation methods, Core B will create an infrastructure and environment for adapting these evolving technologies to the needs of the PPG investigators. All of the essential equipment and expertise required for this core are already in place and available at our institution. Key personnel with the necessary background and expertise have been recruited from existing faculty and staff, and these individuals have been designated appropriate levels of effort to ensure proper functioning of Core B. While some of the equipment and personnel associated with the core are physically housed and partially supported within our NCI CCSG (P30) funded Flow Cytometry Core Facility, the creation of Core B is essential to the successful accomplishment of the goals of the PPG, as it will enable the implementation of these technologies for new and innovative applications that specifically address the studies of cancer metastasis that form the theme of the current program application. PHS 398/2590 (Rev. 11/07) Page

核心 B – 流式细胞术和细胞分选核心（核心负责人：Porcelli）项目负责人：Backer, Jonathan M. 抽象的流式细胞术分析和细胞分选核心（核心 B）将提供设备和专业知识用于单细胞水平分析和分离的各种基于流式细胞术的先进技术。 core 将支持和维护一系列用于分析和分类应用的先进仪器，并将积极指导项目研究人员将这些应用到癌症转移的研究中。方法将用于感兴趣的细胞类型的识别、分离和详细分析核心资源将主要用于所有项目。包括该计划，以及他们在此结构下的组织和支持将为该计划提供核心关键人员将以经济有效的方式获得访问权和技术支持。致力于直接协助项目 PI 开发和实施单一项目与他们的项目相关的细胞分析技术，并帮助他们利用通过为基于流式细胞术的单细胞组合提供组织核。分析和隔离方法，Core B 将创建一个基础设施和环境来适应这些不断发展的技术以满足 PPG 研究人员的需求。我们的机构已经配备了该核心所需的关键人员和必要的人员。背景和专业知识是从现有的教职员工中招募的，这些人已指定适当水平的努力，以确保核心 B 的正常运作。与核心相关的设备和人员实际安置在我们的内部并得到部分支持 NCI CCSG (P30) 资助的流式细胞术核心设施，核心 B 的创建对于成功至关重要实现 PPG 的目标，因为它将能够在新的领域实施这些技术以及专门针对癌症转移研究的创新应用程序，这些研究构成了主题当前程序应用程序。 PHS 398/2590（修订版。11/07）页码