Single Cell Spatial Analysis in Tissue

组织中的单细胞空间分析

• 批准号: 10044479
• 负责人: Jared Kyle Burks
• 金额: $ 25.84万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-09 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10044479
• 关键词: Biological Assay; Biological Sciences; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cell Communication; Cell Separation; Cells; Collaborations; Complex; Comprehensive Cancer Center; Consultations; Core Facility; Cytometry; Education; Ensure; Equipment; Experimental Designs; Faculty; Flow Cytometry; Growth; Hour; Image; Immune; Industry; Methodology; Microscope; Modeling; Process; Proteomics; Publications; Reporting; Research; Research Personnel; Services; Source; Standardization; Suspensions; Technical Expertise; Technology; Testing; Tissues; Training; University of Texas M D Anderson Cancer Center; anticancer research; cancer genomics; cellular imaging; flexibility; imaging facilities; imaging system; instrumentation; liquid crystal polymer; member; neoplastic cell; new technology; novel; programs; single cell technology; stem; success; tool

Project Summary/Abstract Flow Cytometry, Mass Cytometry, Cell Sorting, and Imaging are essential tools for a wide range of methodologies aimed at understanding complex single cell proteomic and genomic cancer research. The instrumentation involved is large, complex, and can be difficult to maintain and operate. As such, a targeted core facility is critical for a Comprehensive Cancer Center such as MD Anderson. The FCCIF, est. 1982, provides these services. Since the last CCSG renewal (2012) 404 cancer center members from all 16 cancer center programs have used the facility and produced 604 publications. Many of the hot topics in cancer biology currently stem from immune cell interaction with the tumor cell; immunephenotyping is an in demand field of research that requiring a unique high plex assay. This requires a specialized imaging or cytometry approach and the FCCIF has specifically acquired two different platforms because of their diversity and flexibility. These platforms are the Vectra Multispectral imaging system and the CyTOF (suspension and imaging) Mass Cytometers. The FCCIF provides a common source of state-of-the-art expertise and technical skills to support the varied research efforts of MD Anderson investigators. The FCCIF provides a comprehensive service to all of the institutional investigators regardless of prior expertise. Dr. Jared K. Burks (author of this R50, co-Director of the FCCIF-NC) provides critical services including: 1) education, 2) consultation on needed technological approach, 3) training on equipment use, analytics, and proper experimental design. I identify and acquire novel technologies for use by MDACC investigators and I provide support in a manner to ensure success and mitigate pitfalls. I am expected to develop new strategies and technologies to meet the research needs of our faculty. A few examples of what I have currently been working on is the Vectra Polaris Multispectral Microscope, Helios and Hyperion Suspension and Imaging CyTOFs. The Polaris has operated on average 13.5 hours per day for the last year. The CyTOFs are reporting 5090% growth since the last CCSG renewal. These results stem from mitigating the pitfalls, making the technologies approachable and affordable, and supporting the researchers throughout the process all the way to publication. Collaborations, both academic and industry, have resulted to develop or test novel applications. The models we have created to support these technologies have and will continue to be shared with others operating the technologies. We are highly involved with both developing and standardizing new technologies for use at MD Anderson and with our facilities and researchers implementing them worldwide.

项目摘要/摘要 流式细胞仪，质量细胞术，细胞分类和成像是多种方法的必不可少的工具 旨在了解复杂的单细胞蛋白质组学和基因组癌研究。仪器 涉及的很大，复杂，很难维护和操作。因此，有针对性的核心设施至关重要 对于MD Anderson等综合癌症中心。 FCCIF，Est。1982提供了这些服务。 自上次CCSG续订以来（2012年）404位来自所有16个癌症中心计划的癌症中心成员 该设施并生产了604个出版物。癌症生物学的许多热门主题目前来自免疫 与肿瘤细胞相互作用；免疫原子型是一个需求领域，需要独特 高PLEX分析。这需要专门的成像或细胞仪方法，FCCIF具有专门的 由于它们的多样性和灵活性，因此获得了两个不同的平台。这些平台是vectra 多光谱成像系统和细胞（悬架和成像）质量细胞仪。 FCCIF提供的 最先进的专业知识和技术技能的共同来源，以支持医学博士的各种研究工作 安德森调查人员。 FCCIF为所有机构调查人员提供全面的服务 不管先前的专业知识如何。 Jared K. Burks博士（该R50的作者，FCCIF-NC的联合主任）提供 关键服务包括：1）教育，2）就所需的技术方法进行咨询，3）培训 设备使用，分析和适当的实验设计。我识别并获取了新颖的技术，以供使用 我和MDACC的调查人员提供了一种支持，以确保成功并减轻陷阱。我是期望的 制定新的策略和技术以满足我们教师的研究需求。几个例子 我目前一直在研究Vectra Polaris多光谱显微镜，Helios和Hyperion悬架 和成像细胞。去年，北极星平均每天运行13.5小时。细胞 自上次CCSG续签以来，报告的增长率为5090％。这些结果源于减轻陷阱，使 这些技术平易近人和负担得起，并在整个过程中支持研究人员 出版方式。学术和行业的合作已导致开发或测试小说 申请。我们为支持这些技术所创建的模型而将继续共享 与其他人一起运营技术。我们高度参与开发和标准化新的 MD Anderson以及我们的设施和研究人员在全球实施它们的技术。