Cytometry Core

细胞计数核心

基本信息

批准号：
10588840
负责人：
Remi J Creusot
金额：
$ 13.62万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
起止时间：
2002-09-01 至 2027-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588840
关键词：
Address Adipose tissue Alpha Cell Apoptosis Beta Cell CRISPR screen Cancer Center Cell Cycle Cell Proliferation Cell Separation Cell surface Cells Center Core Grants Chimeric Proteins Collection Complex Core Facility Cytometry Dedications Development Diabetes Mellitus Disease model Dyes Eligibility Determination Endocytosis Equipment Flow Cytometry Flow Cytometry Shared Resource Fluorescence-Activated Cell Sorting Frequencies Gene Expression Profiling Genes Grant Hepatocyte Herbert Irving Comprehensive Cancer Center Housing Immune Immunologics In Vitro Individual Institution Insulin-Dependent Diabetes Mellitus Investments Islet Cell Lipids Liver Lymphoid Cell Lymphoid Tissue Lysosomes Measurement Measures Medicine Metabolic Mitochondria Modeling Molecular Monitor Neurons Organelles Organoids Oxidative Stress Pancreas Pathway interactions Phenotype Physiological Ploidies Population Procedures Proliferating Quality Control Reagent Receptor Signaling Reproducibility Research Research Personnel Resolution Role S10 grant Sampling Services Signal Transduction T-Cell Activation Techniques Technology Therapeutic Tissues United States National Institutes of Health Universities animal tissue antibody conjugate base cell type cost cytokine design engineered stem cells epigenetic profiling experience experimental study flexibility fluorescence imaging genetic profiling human tissue humanized mouse implantation in vivo instrument instrumentation mRNA Expression member migration mouse model new technology programs protein protein interaction protein purification release of sequestered calcium ion into cytoplasm stem cells tool transcription factor translational immunology

项目摘要

Flow cytometry is an important tool to quantify and phenotype cells that define pathways relevant to diabetes. Previously limited to the analysis of circulating immune cells, advances in cell extraction from tissues has allowed flow cytometry to expand its capabilities to the study of developmental, metabolic and signaling programs in a wide variety of cell types. The Cytometry & Cell Sorting Core (CCSC) is a vital component of the Columbia University Diabetes Research Center (DRC), as it addresses the research needs of many DRC investigators who study immunological, developmental, and metabolic aspects of diabetes. The Core was created in 2010 in partnership with the Columbia Center for Translational Immunology and the Department of Medicine, with substantial University investment as well as NIH support (S10 and P30 grants) and expanded in 2017 by incorporating the Cancer Center’s flow cytometry core. The core expansion from 5 to 11 instruments (4 coming from the Cancer Center and 2 new spectral flow cytometers) has provided greater choice and flexibility for DRC members. We expect DRC member usage of the core to remain steady and strong, given attractive new technologies afforded by spectral flow cytometry. CCSC will assist investigators in two ways: Aim 1 – To quantify and phenotypically characterize cell populations that contribute to the metabolic, immunological, and developmental programs of diabetes and its complications. CCSC will leverage advanced technologies in fluorescent imaging at the single cell resolution to support the analysis of different cell types in human and animal tissues, including humanized mouse models of Type 1 diabetes. Aim 2 – To purify populations of cells of relevance to diabetes and its complications. Using fluorescence-activated cell sorting, the CCSC will support DRC investigators to purify individual populations of cells for culture, in vivo implantation, or molecular characterization including genetic and transcriptional profiling, protein purification and signaling studies, and for functional analysis including T cell activation, proliferation, and migration studies. To achieve these aims, the CCSC has established standard operating procedures to: (i) assure quality control and reproducibility; (ii) prioritize investigator use; (iii) monitor Core usage; and (iv) adapt to new technologies and to the needs of the Columbia Research Base.

流式细胞仪是定义与糖尿病相关的途径的量化和表型细胞的重要工具。目前仅限于分析循环免疫细胞的分析，从组织中提取细胞的进展允许流动的细胞仪扩大其能力，以研究发育，代谢和信号传导各种细胞类型的程序。哥伦比亚大学糖尿病研究中心（DRC），因为它解决了许多刚果民主共和国的研究需求研究糖尿病的免疫，发育和代谢方面的研究者与哥伦比亚转化免疫学中心合作创建于2010年医学，具有大量的大学投资以及NIH的支持（S10和P30赠款），并扩大了 2017年通过融合了癌症中心的流式细胞计核。（4来自癌症中心和2个新的光流动细胞仪）证明了更大的选择，并且 DRC成员的灵活性。光谱细胞仪提供的有吸引力的新技术将以两种方式帮助调查人员：目标1-量化和表型表征有助于代谢，免疫学和糖尿病的发展计划及其并发症。荧光成像中的技术在单一celle celle celle细胞分辨率分析以分析不同细胞类型中的技术人类和动物组织，包括1型糖尿病的人源化小鼠模型。与糖尿病相关的细胞及其并发症。 CCSC将支持DRC研究人员在体内净化细胞的单个细胞种群以供培养植入或分子表征，包括遗传和转录分析，蛋白质纯化和信号研究，以及用于功能分析，包括T细胞激活，促进性研究。为了实现这些目标，CCSC已建立了标准操作程序：（i）确保质量控制和繁殖；（ii）优先使用研究者；以及满足哥伦比亚研究基础的需求。