Biomarkers of Caloric Restriction in Humans: the CALERIE Biorepository

人类热量限制的生物标志物：CALERIE 生物存储库

• 批准号: 9278061
• 负责人: WILLIAM E KRAUS
• 金额: $ 60.78万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9278061
• 关键词: Address; Aging; Ancillary Study; Attention; Award; Biological; Biological Markers; Biological Process; Biology; Biology of Aging; Biopsy Specimen; Blood specimen; Caloric Restriction; Clinical Data; Clinical Research; Collection; Communities; Complex; Controlled Study; Coupling; DNA; DNA Methylation; Data; Databases; Diet; Energy Intake; Epigenetic Process; FGF21 gene; Fatty acid glycerol esters; Funding; Future; Gene Expression; Genetic; Health; Human; Insecta; Intervention; Investigation; Left; Link; Longevity; Maintenance; Mediating; Metabolism; Molecular; Muscle; Nature; Organism; Overweight; Participant; Phase; Physiological; Publications; Randomized; Randomized Controlled Trials; Request for Proposals; Research Personnel; Resources; Rodent; Role; Sampling; Sampling Studies; Science; Site; Skeletal Muscle; Study Subject; Surrogate Markers; Systems Biology; Time; Tissues; Uncontrolled Study; Universities; Urine; Vermont; Weight; Yeasts; biobank; biological systems; cardiometabolism; clinical data warehouse; data warehouse; design; human study; human subject; metabolic profile; nonhuman primate; peripheral blood; public health relevance; repository

 DESCRIPTION (provided by applicant): Aging is a complex biological process, involving all tissues of the body and their interactions through metabolism and genetic biology. It has been known for some time that reducing caloric intake can promote extension of median and maximal lifespan across a broad range of eukaryotic organisms from yeast, through insects, worms, rodents and nonhuman primates. There have been numerous controlled and uncontrolled studies of the effects of caloric restriction on surrogate biomarkers of aging lasting up to six months, but the effects of longer term calorie restriction notably have been absent until the inception of the CALERIE study. The CALERIE study was designed to assess the effects of two years of sustained and significant caloric restriction without enforced changes of dietary composition in human subjects in a randomized controlled trial. The duration of the intervention and the randomized nature of the treatment assignment had never previously been attempted in a human study. These factors, combined with the substantial size of the study, the careful attention to detail in the physiologic assessments, careful collection of extensive clinical data, and the extensive biorepository of samples collected from study participants, make the biorepository an invaluable resource for the investigation of the systematic molecular biological effects of medium term caloric restriction in human subjects. With this award, we will support the following projects for five years: maintenance of the data repository at Duke University, maintenance of the biorepository at the University of Vermont, the ability to link clinical data wih biological samples, and the committee of CALERIE investigators who steward use of the samples. This repository will provide access to collected data and samples to present and future investigators investigating caloric restriction in humans. In addition, we will conduct preliminary systems biological investigations of the effects of caloric restriction using the biorepository samples. The Specific Aims are: 1) Maintenance of the CALERIE Data Repository and Biological Sample Repository; 2) Stewardship of applications for use of the CALERIE Sample Repository; 3) Address secondary questions regarding the biology of calorie restriction in humans. of current CALERIE data; 4) Curation of the CALERIE Database with molecular studies to promote a systems biology approach to the understanding of calorie restriction in humans. At the end of this funding period we will have available a rich biological and clinical data repository available to the scientific community for the investigation of innumerable hypotheses about the role of calorie restriction on the human aging biology.

 描述（由申请人提供）：衰老是一个复杂的生物过程，涉及身体的所有组织及其通过新陈代谢和遗传生物学的相互作用，一段时间以来，人们已经知道减少热量摄入可以促进中位寿命和最大寿命的延长。从酵母到昆虫、蠕虫、啮齿动物和非人类灵长类动物，范围广泛的真核生物中，已经有许多关于热量限制对衰老替代生物标志物的影响的受控和非受控研究。六个月，但直到 CALERIE 研究开始之前，长期热量限制的显着效果一直不存在。 CALERIE 研究旨在评估在不强制改变膳食成分的情况下持续两年的显着热量限制对人类受试者的影响。在一项随机对照试验中，干预的持续时间和治疗分配的随机性质以前从未在人体研究中尝试过，再加上研究的规模很大，以及对生理评估细节的仔细关注。 ，精心收集大量临床资料数据以及从研究参与者收集的广泛生物样本库，使该生物样本库成为研究人类受试者中期热量限制的系统性分子生物学效应的宝贵资源。凭借这一奖项，我们将在五年内支持以下项目。 ：杜克大学数据存储库的维护、佛蒙特大学生物存储库的维护、将临床数据与生物样本联系起来的能力以及管理样本使用的 CALERIE 研究人员委员会将提供访问权限。为当前和未来研究人类热量限制的研究人员收集数据和样本。此外，我们还将进行初步研究。 使用生物样本库对热量限制的影响进行系统生物学研究。 具体目标是： 1) 维护 CALERIE 数据存储库和生物样本存储库； 2) 管理 CALERIE 样本存储库的使用申请； 3) 解决次要问题。关于人类热量限制的生物学；4) 通过分子研究整理 CALERIE 数据库，以促进系统生物学方法的理解。在此资助期结束时，我们将为科学界提供丰富的生物学和临床数据存储库，用于调查有关热量限制对人类衰老生物学的作用的无数假设。