Biomarkers of Caloric Restriction in Humans: the CALERIE Biorepository

人类热量限制的生物标志物：CALERIE 生物存储库

• 批准号: 9278061
• 负责人: WILLIAM E KRAUS
• 金额: $ 60.78万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9278061
• 关键词: Address; Aging; Ancillary Study; Attention; Award; Biological; Biological Markers; Biological Process; Biology; Biology of Aging; Biopsy Specimen; Blood specimen; Caloric Restriction; Clinical Data; Clinical Research; Collection; Communities; Complex; Controlled Study; Coupling; DNA; DNA Methylation; Data; Databases; Diet; Energy Intake; Epigenetic Process; FGF21 gene; Fatty acid glycerol esters; Funding; Future; Gene Expression; Genetic; Health; Human; Insecta; Intervention; Investigation; Left; Link; Longevity; Maintenance; Mediating; Metabolism; Molecular; Muscle; Nature; Organism; Overweight; Participant; Phase; Physiological; Publications; Randomized; Randomized Controlled Trials; Request for Proposals; Research Personnel; Resources; Rodent; Role; Sampling; Sampling Studies; Science; Site; Skeletal Muscle; Study Subject; Surrogate Markers; Systems Biology; Time; Tissues; Uncontrolled Study; Universities; Urine; Vermont; Weight; Yeasts; biobank; biological systems; cardiometabolism; clinical data warehouse; data warehouse; design; human study; human subject; metabolic profile; nonhuman primate; peripheral blood; public health relevance; repository

 DESCRIPTION (provided by applicant): Aging is a complex biological process, involving all tissues of the body and their interactions through metabolism and genetic biology. It has been known for some time that reducing caloric intake can promote extension of median and maximal lifespan across a broad range of eukaryotic organisms from yeast, through insects, worms, rodents and nonhuman primates. There have been numerous controlled and uncontrolled studies of the effects of caloric restriction on surrogate biomarkers of aging lasting up to six months, but the effects of longer term calorie restriction notably have been absent until the inception of the CALERIE study. The CALERIE study was designed to assess the effects of two years of sustained and significant caloric restriction without enforced changes of dietary composition in human subjects in a randomized controlled trial. The duration of the intervention and the randomized nature of the treatment assignment had never previously been attempted in a human study. These factors, combined with the substantial size of the study, the careful attention to detail in the physiologic assessments, careful collection of extensive clinical data, and the extensive biorepository of samples collected from study participants, make the biorepository an invaluable resource for the investigation of the systematic molecular biological effects of medium term caloric restriction in human subjects. With this award, we will support the following projects for five years: maintenance of the data repository at Duke University, maintenance of the biorepository at the University of Vermont, the ability to link clinical data wih biological samples, and the committee of CALERIE investigators who steward use of the samples. This repository will provide access to collected data and samples to present and future investigators investigating caloric restriction in humans. In addition, we will conduct preliminary systems biological investigations of the effects of caloric restriction using the biorepository samples. The Specific Aims are: 1) Maintenance of the CALERIE Data Repository and Biological Sample Repository; 2) Stewardship of applications for use of the CALERIE Sample Repository; 3) Address secondary questions regarding the biology of calorie restriction in humans. of current CALERIE data; 4) Curation of the CALERIE Database with molecular studies to promote a systems biology approach to the understanding of calorie restriction in humans. At the end of this funding period we will have available a rich biological and clinical data repository available to the scientific community for the investigation of innumerable hypotheses about the role of calorie restriction on the human aging biology.

 描述（由适用提供）：衰老是一个复杂的生物学过程，涉及身体的所有组织及其通过代谢和遗传生物学的相互作用。一段时间以来，人们已经知道减少热量摄入可以促进中位数和最大寿命的延伸，从酵母，昆虫，蠕虫，啮齿动物和非人类隐私。关于热量限制对持续六个月的衰老的替代生物标志物的影响，已经进行了许多受控和不受控制的研究，但是直到卡路里研究开始时，长期卡路里限制的影响才显得鲜明。 Calerie研究旨在评估在随机对照试验中，在人类受试者中执行饮食组成的持续和严重限制的效果。在人类研究中，干预措施的持续时间和治疗分配的随机性质从未尝试过。这些因素，结合了研究的实质性大小，对物理评估中细节的仔细关注，仔细收集了广泛的临床数据以及从研究参与者收集的样本的广泛生物位，使生物座位成为对人类受试者中等学期限制的系统性分子生物学限制的无价资源。通过该奖项，我们将支持以下项目五年：维护杜克大学的数据存储库，维护佛蒙特大学的生物库，可以将临床数据与生物学样本联系起来的能力，以及稳定样品使用样品的Calerie调查人员委员会。该存储库将提供对收集的数据和样本的访问，以介绍和未来的调查人员调查人类热量限制。此外，我们将进行初步 系统生物学投资对使用生物座的样品对热量限制的影响。具体目的是：1）维护热量数据存储库和生物样品存储库； 2）使用Calerie样品存储库的应用程序； 3）解决有关人类卡路里限制生物学的次要问题。当前的热量数据； 4）使用分子研究策划卡利亚数据库，以促进一种系统生物学方法来理解人类卡路里的限制。在此资助期结束时，我们将获得科学界的丰富生物和临床数据存储库，以调查有关卡路里限制对人类衰老生物学的作用的无数假设。