Ophthalmology Core Facility

眼科核心设施

基本信息

批准号：
9129695
负责人：
JOHN C MORRISON
金额：
$ 77万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-05-01 至 2020-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9129695
关键词：
Affect Anterior Biometry Cataract Cluster Analysis Collaborations Communities Complex Confocal Microscopy Core Facility DNA Data Data Analyses Development Disease Equipment Eye Faculty Fostering Funding Gene Expression Gene Expression Profile Gene Silencing Gene Transfer Genes Genetic Glaucoma Goals Grant Health Sciences Human Resources Image Immunofluorescence Microscopy Individual Life Macular degeneration Mass Spectrum Analysis Mentors Methods Modeling Molecular Genetics Ophthalmology Optic Nerve Oregon Patients Physiological Proteins Proteomics RNA Interference Research Research Design Research Personnel Resource Sharing Resources Retina Rodent Sampling Scientist Series Services Techniques Tissue Sample Tissues Trabecular meshwork structure Training Transcriptional Regulation Transfection United States National Institutes of Health Universities Uveitis Viral Genes Vision Vision research Work bioimaging career gene discovery genetic variant in vivo imaging inherited retinal degeneration lens mutant overexpression professional atmosphere protein complex protein structure public health relevance ranpirnase response transcription factor

项目摘要

DESCRIPTION (provided by applicant): This P30 Ophthalmology Core Facility renewal application is submitted to provide support for NEI-funded Oregon Health and Science University (OHSU) vision researchers in four Resource cores: Bio-Imaging & Confocal Microscopy (Bio-Imaging); Gene Expression & Manipulation (Gene Manipulation); Molecular Genetics & Biostatistics (Genetics/Biostats); and Proteomics & Mass Spectrometry (Proteomics). These shared resources will provide equipment and personnel that would otherwise not be available to individual researchers working in a diverse array of diseases affecting nearly all parts of the eye, including the anterior segment, trabecular meshwork, lens, retina, and optic nerve. Associated diseases include glaucoma, inherited retinal degenerations, macular degeneration, cataract, uveitis and the genetics of glaucoma and macular degeneration. Bio-Imaging will support immunofluorescence microscopy for identification and localization of proteins within tissues, as well as provide support for a new module using Spectral Domain OCT for in vivo imaging of rodent eyes. Gene manipulation will provide technical support and advice for a range of methods to identify changes in levels of gene expression and their products (proteins) that result either from disease states or their treatment, and for methods by which these responses can be manipulated, such as RNAi silencing, gene overexpression or gene variant/mutant expression via transfection or viral gene transfer agents. Methods for studying transcriptional regulation will also be available. Genetics/Biostats will provide DNA isolation services from patient samples to NEI-funded investigators, and will provide a wide range of biostatistical support, including advice on study design, data analysis, and expert assistance with sophisticated methods such as high dimensional data, advanced clustering analysis and transcription factor prediction from microarray data. The Proteomics core will provide access to advanced techniques for probing protein structure with higher sensitivity, throughput and accuracy. All 4 cores are highly complementary and, in combination with a departmental vision research seminar series, will foster a positive atmosphere of collaborative vision research.

描述（由申请人提供）：提交此 P30 眼科核心设施更新申请的目的是为 NEI 资助的俄勒冈健康与科学大学 (OHSU) 视觉研究人员提供以下四个资源核心的支持：生物成像和共聚焦显微镜（生物成像）；基因表达和操作（基因操作）；以及蛋白质组学和质量；这些共享资源将为研究影响眼睛几乎所有部位（包括眼前节、小梁网、晶状体、视网膜和眼部）的各种疾病的个体研究人员提供无法获得的设备和人员。相关疾病包括青光眼、遗传性视网膜变性、黄斑变性、白内障、葡萄膜炎以及青光眼和黄斑变性的遗传学将支持免疫荧光。用于识别和定位组织内蛋白质的显微镜，以及为使用光谱域 OCT 进行啮齿动物眼睛体内成像的新模块提供支持基因操作将为一系列识别蛋白质水平变化的方法提供技术支持和建议。由疾病状态或其治疗产生的基因表达及其产物（蛋白质），还可以提供用于研究转录调控的方法，例如 RNAi 沉默、基因过表达或通过转染或病毒基因转移剂的基因变异/突变体表达。从患者样本到 NEI 资助的研究人员，并将提供广泛的生物统计支持，包括研究设计建议、数据分析以及使用高维数据、高级聚类分析和转录因子预测等复杂方法的专家协助蛋白质组学核心将提供以更高的灵敏度、通量和准确性探测蛋白质结构的先进技术，所有 4 个核心具有高度互补性，并与部门视觉研究研讨会系列相结合，将营造积极的协作氛围。视觉研究。