Ophthalmology Core Facility

眼科核心设施

基本信息

批准号：
10707492
负责人：
JOHN C MORRISON
金额：
$ 76.75万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-05-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10707492
关键词：
Academic support Age Angiography Animal Model Bioinformatics Biological Biometry Biostatistics Core Blood specimen CRISPR/Cas technology Cataract Cell Culture Techniques Cells Cellular biology Childhood Clinical Clinical Research Clinical Trials Collaborations Communication Communities Complex Confocal Microscopy Core Facility Core Grant DNA Data Analyses Data Set Development Diabetic Retinopathy Disease Ensure Equipment Eye Eye diseases Faculty Family suidae Fibroblasts Funding Gene Chips Gene Expression Gene Mutation Gene Proteins Genes Genetic Genetic Models Genotype Glaucoma Goals Grant Health Human Human Resources Image Imaging technology Individual Inherited Macular degeneration Maintenance Measures Mentored Clinical Scientist Development Program Mentors Methods Modeling Modification Molecular Molecular Genetics Mus Ophthalmology Optical Coherence Tomography Oregon Organ Culture Techniques Patients Phenotype Physiology Population Primary Cell Cultures Primates Proteins Proteomics RNA Interference Rattus Research Research Design Research Infrastructure Research Personnel Resolution Resource Sharing Resources Retinal Diseases Rodent Saliva Sampling Science Scientist Services Techniques Tissue Sample Tissues Training Universities Uveitis Vision Vision research Work animal imaging bench to bedside bioimaging career design detection method gene discovery genetic manipulation in vivo imaging instrumentation microscopic imaging nonhuman primate overexpression patient population precision medicine programs protein folding research study response retinal imaging tissue culture transcriptome sequencing

项目摘要

OVERALL CORE PROJECT SUMMARY This P30 Ophthalmology Core Facility provides ongoing support for NEI-funded Oregon Health and Science University (OHSU) and Casey Eye Institute vision researchers. The four resource cores are: Bioimaging & Confocal Microscopy; Gene Expression & Manipulation; Genetic Models of Ocular Disease & Biostatistics; and Proteomics. These shared resources will provide equipment and personnel otherwise not available to individual researchers working in a wide range of vision-threatening diseases, including cataracts, glaucoma, macular degeneration, diabetic retinopathy, uveitis, pediatric eye disease, the physiology of vision and the genetics of glaucoma, macular degeneration, uveitis and inherited retinal diseases. The Bioimaging & Confocal Microscopy core will support confocal microscopy studies using state-of-the-art instrumentation for identification and high- resolution localization of proteins. In addition, this core will continue to support small animal imaging through maintenance of a Micron IV Retinal Imaging Microscope for in vivo imaging of rodent eyes. The Gene Expression & Manipulation core will provide instrumentation and technical support for a range of molecular methods to identify changes in levels of gene expression and proteins, and for methods by which these responses can be manipulated, such as RNAi silencing, gene overexpression or gene editing by CRISPR/Cas9. The Genetic Models of Ocular Disease & Biostatistics core (formerly Molecular Genetics & Biostatistics) will continue to provide DNA isolation services from patient blood samples, saliva and tissue and provide access for NEI investigators to advanced statistical techniques to ensure use of appropriate methods both in study design and for data analysis. Biostatistical services include analysis of complex gene expression arrays and RNA-seq datasets, large proteomics studies, optical coherence tomography (OCT) and OCT angiography studies and large patient population data sets from bioinformatics and clinical research studies. Two new services will provide (1) genotyping of cell, tissue and biological samples from human, non-human primate, rat, mouse and pig tissues and (2) provide assistance in establishing primary cell cultures from ocular tissues, as well as fibroblasts from patients with ocular disease. Offering these new services will enable functional genotype-phenotype studies for ocular disease, a critical enhancement in the current age of precision medicine. The Proteomics core will provide access to advanced, high-throughput techniques for measuring changes in protein abundance and modification with disease, determining how proteins fold and interact with one another, and how they regulate development. All four cores are highly complementary and, in combination with new programs designed to encourage communication between clinicians and basic scientists, will increase discoveries with greater direct benefit to patients.

核心项目总体概要这个 P30 眼科核心设施为 NEI 资助的俄勒冈州健康与科学提供持续支持大学 (OHSU) 和凯西眼科研究所的视力研究人员。四个资源核心是：生物成像和共焦显微镜；基因表达与操作；眼部疾病遗传模型与生物统计学；和蛋白质组学。这些共享资源将提供个人无法获得的设备和人员研究人员致力于研究各种威胁视力的疾病，包括白内障、青光眼、黄斑退化、糖尿病视网膜病变、葡萄膜炎、小儿眼病、视觉生理学和遗传学青光眼、黄斑变性、葡萄膜炎和遗传性视网膜疾病。生物成像和共焦显微镜核心将支持使用最先进的仪器进行共焦显微镜研究，以进行识别和高蛋白质的分辨率定位。此外，该核心将继续支持小动物成像维护 Micron IV 视网膜成像显微镜，用于啮齿动物眼睛的体内成像。基因表达 & Manipulation core将为一系列分子方法提供仪器和技术支持识别基因表达和蛋白质水平的变化，以及可用于这些反应的方法操纵，例如 RNAi 沉默、基因过表达或通过 CRISPR/Cas9 进行基因编辑。遗传基因眼部疾病模型和生物统计学核心（以前的分子遗传学和生物统计学）将继续提供从患者血液样本、唾液和组织中分离 DNA 的服务，并为 NEI 提供访问权限研究人员采用先进的统计技术，以确保在研究设计和研究中使用适当的方法用于数据分析。生物统计服务包括复杂基因表达阵列和 RNA-seq 的分析数据集、大型蛋白质组学研究、光学相干断层扫描 (OCT) 和 OCT 血管造影研究以及来自生物信息学和临床研究的大量患者群体数据集。将提供两项新服务 (1) 对人类、非人灵长类动物、大鼠、小鼠和猪组织的细胞、组织和生物样品进行基因分型 (2) 协助建立眼组织的原代细胞培养物以及来自眼组织的成纤维细胞患有眼部疾病的患者。提供这些新服务将使功能基因型-表型研究成为可能眼部疾病是当今精准医学时代的一个重要进步。蛋白质组学核心将提供获得先进的高通量技术来测量蛋白质丰度和修饰的变化与疾病相关，确定蛋白质如何折叠和相互作用，以及它们如何调节发育。所有四个核心都具有高度互补性，并与旨在鼓励临床医生和基础科学家之间的交流将增加发现，从而为人类带来更大的直接利益患者。

项目成果

期刊论文数量（768）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

KHARON1 mediates flagellar targeting of a glucose transporter in Leishmania mexicana and is critical for viability of infectious intracellular amastigotes.

KHARON1 介导墨西哥利什曼原虫中葡萄糖转运蛋白的鞭毛靶向，对于感染性细胞内无鞭毛体的活力至关重要。

DOI：
10.1074/jbc.m113.483461
发表时间：
2013
期刊：
The Journal of biological chemistry
影响因子：
0
作者：
Tran,KhoaD;Rodriguez-Contreras,Dayana;Vieira,DanielleP;Yates,PhillipA;David,Larry;Beatty,Wandy;Elferich,Johannes;Landfear,ScottM
通讯作者：
Landfear,ScottM

PSEUDOPANUVEITIS AS A HARBINGER FOR SYSTEMIC LEUKEMIA RECURRENCE.

假性泛葡萄膜炎是系统性白血病复发的先兆。

DOI：
10.1097/icb.0000000000001172
发表时间：
2023
期刊：
Retinal cases & brief reports
影响因子：
0
作者：
Altman,MarcusT;Staubach,Zane;Dunlap,Jennifer;Zaki,Amr;Skalet,AlisonH;Leonard,Jessica;Lin,Phoebe
通讯作者：
Lin,Phoebe

Decreased Vision and Eye Pain in a 55-Year-Old Man.

55 岁男性视力下降和眼睛疼痛。

DOI：
10.1001/jamaophthalmol.2021.4447
发表时间：
2022
期刊：
JAMA ophthalmology
影响因子：
8.1
作者：
Yee,Claudine;Chamberlain,Winston;Thomas,Merina
通讯作者：
Thomas,Merina

Impact of the COVID-19 pandemic on uveitis patient care.

COVID-19 大流行对葡萄膜炎患者护理的影响。

DOI：
10.1136/bjophthalmol-2021-320368
发表时间：
2023
期刊：
The British journal of ophthalmology
影响因子：
0
作者：
Vu,AlexanderF;Kodati,Shilpa;Lin,Phoebe;Bodaghi,Bahram;Emami-Naeini,Parisa;COVID-19PracticePatternsStudyGroup
通讯作者：
COVID-19PracticePatternsStudyGroup

Incidence of retinal detachment after fellow-performed primary pars plana vitrectomy.