X-ray data analysis in the presence of structural variability

存在结构变异时的 X 射线数据分析

• 批准号: 9552204
• 负责人: WLADEK MINOR
• 金额: $ 33.52万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-22 至 2020-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9552204
• 关键词: Address; Affect; Automation; Behavior; Biological; Biological Process; Cell physiology; Characteristics; Communities; Complex; Complication; Computer software; Crystallization; Data; Data Analyses; Data Collection; Data Quality; Data Set; Descriptor; Dimensions; Disease; Drug Design; Foundations; Health; Individual; Internet; Investigation; Ligand Binding; Ligands; Maps; Measures; Methodology; Methods; Mission; Modeling; Molecular; Morphologic artifacts; Noise; Online Systems; Outcome; Pharmaceutical Preparations; Phase; Procedures; Property; Radiation-Induced Change; Research Design; Research Personnel; Residual state; Roentgen Rays; Sampling; Signal Transduction; Software Tools; Source; Specimen; Speed; Structural Models; Structure; System; Techniques; Temperature; Uncertainty; United States National Institutes of Health; Validation; Weight; Work; X ray diffraction analysis; X-Ray Crystallography; base; combinatorial; computerized data processing; data space; data structure; design; detector; electron density; experience; experimental study; innovation; interest; method development; novel; novel strategies; public health relevance; structural biology; tool

 DESCRIPTION (provided by applicant): The proposal "X-ray data analysis in the presence of structural variability" aims to advance diffraction data analysis methods so that the variability between crystals and within crystals is optimally modeled during data processing in reciprocal space and during structural analysis in real space. The significance of the proposed work results from the importance of the technique, which generates uniquely-detailed information. X-ray structures are used to understand cellular processes at the atomic level directly, to explain and validate results obtain by other techniques, to generate hypotheses for detailed studies of cellular process, and to guide drug design studies - all of which are highly relevant to the NIH mission. Macromolecular crystals are frequently of limited size and crystal lattice order. Both may result in the need for combining data from multiple crystals for successful structure solution, with the limited order generating diffraction artifacts and correlating with non-isomorphism between different specimens. Non-isomorphism hinders the averaging of data sets from multiple crystals, because for successful averaging, data need to be very similar. The problems with averaging are compounded by incompleteness of the data in a single data set, radiation-induced changes in the crystal under investigation, and lack of statistical measures that would inform experimenters regarding whether or not the data analysis is progressing in the right direction. There are also technical challenges associated with averaging multiple data sets that result from the combinatorial complexity of data analysis when a large number of data sets need to be analyzed. Final difficulty appears when the analysis of the structural results obtained from multi-crystal experiments must separate the desired biological signals, e.g. the presence of a ligand or a specific dynamic behavior of the molecules, from the noise. Our proposal addresses these problems by developing and implementing innovative approaches. In Aim 1, new approaches will be developed and implemented for averaging multiple, potentially incomplete data sets resulting from one or more crystals. Owing to our innovative approach to modeling the components of non-isomorphism, we expect that even quite non-isomorphous data sets can be used together to solve challenging structures. In Aim 2, methods that will analyze the results of averaging data sets from multiple crystals in real space will be developed. The descriptors of averaging will be correlated with the outcomes of the structural analysis, so that the contributors to variability in real space can be quantified and interpreted. Finally, in Am 3, a web-based server will be developed in order to provide these methods to the structural biology community.

 描述（由申请人提供）：提案“存在结构变异性的 X 射线数据分析”旨在推进衍射数据分析方法，以便在倒易空间中的数据处理和结构分析过程中对晶体之间和晶体内的变异性进行最佳建模。所提出的工作的重要性源于该技术的重要性，该技术产生独特的详细信息，用于直接理解原子水平的细胞过程，以解释和验证所获得的结果。通过其他技术，为细胞过程的详细研究产生假设，并指导高度药物设计研究 - 所有这些都与 NIH 任务相关。大分子晶体通常具有有限的尺寸和晶格顺序，这两者都可能导致这种需求。用于组合来自多个晶体的数据以获得成功的结构解，具有有限阶生成衍射伪影并与不同样本之间的非同构相关。非同构阻碍了来自多个晶体的数据集的平均，因为为了成功平均，数据。平均的问题因单个数据集中的数据不完整、所研究的晶体中辐射引起的变化以及缺乏可以告知实验者数据分析是否正确的统计措施而变得更加复杂。当需要分析大量数据集时，还存在与平均多个数据集相关的技术挑战，这是由于数据分析的组合复杂性而导致的。多晶实验必须分离出所需的我们的建议通过开发和实施创新方法来解决这些问题，目标 1 将开发和实施新方法来平均多个潜在的信号。由于我们对非同构成分进行建模的创新方法，我们期望即使是完全非同构的数据集也可以一起使用来解决目标 2 中具有挑战性的结构。分析平均结果将开发来自真实空间中多个晶体的数据集，并将其与结构分析的结果相关联，以便可以量化和解释真实空间中变化的因素。将开发基于服务器的服务器，以便向结构生物学界提供这些方法。