Acquisition of rodent metabolic and behavioral phenotyping system

啮齿动物代谢和行为表型系统的获取

• 批准号: 10799014
• 负责人: Kyle Patrick Quinn
• 金额: $ 25万
• 依托单位: UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10799014
• 关键词: Arkansas; Behavioral; Bioenergetics; Biomedical Research; Body Weight; Carbon Dioxide; Cells; Centers of Research Excellence; Consumption; Data; Data Science; Development; Disease; Faculty; Future; Genus Hippocampus; Image; Interdisciplinary Study; Isotopes; Kinetics; Laboratory Animals; Measurement; Metabolic; Metabolism; Molecular; Monitor; Oxygen Consumption; Phase; Production; Research; Research Personnel; Research Project Grants; Resolution; Resources; Rodent; Role; System; Tissues; Universities; behavioral phenotyping; innovation; instrument; laboratory experiment; metabolic phenotype; recruit; repaired; water vapor

PROJECT SUMMARY The Arkansas Integrative Metabolic Research Center (AIMRC) is a Phase I COBRE at the University of Arkansas at Fayetteville (04/01/2021-02/28/2026; PI: Dr. Kyle P. Quinn). The scientific objective of the AIMRC is to investigate the role of cell and tissue metabolism in disease, development, and repair through research involving advanced imaging, bioenergetics, and data science. The long-term objective of the AIMRC is to establish a sustainable interdisciplinary research center that can support biomedical research at the U of A and grow the emerging strengths in metabolic research on campus. The Bioenergetics Core currently assists researchers with measurements of metabolic flux at the cell and isolated tissue levels. This supplement will add complementary new capabilities by enabling non-destructive and automated monitoring of rodents to simultaneously monitor body weight, oxygen consumption, carbon dioxide release, and water vapor formed during the kinetic activities of these laboratory animals through the acquisition of Promethion metabolic cages. The instrument will enable investigators to assess the unusual consumption/accumulation of heavy isotope enriched (13C/15N/2H) metabolites formed under various disease conditions. The data acquired using the Promethion metabolic core is expected to provide valuable information to understand the molecular mechanisms underlying various diseases. The information gained from the Promethion metabolic core can be highly complementary to the data acquired using the other available instrumentational facilities (Seahorse and Oroboros respirometers) available in the AIMRC Bioenergetics Core. The Promethion metabolic cages purchased as part of this supplement request will provide a unique resource to our campus and region. It will be highly beneficial for the research proposed by current research project leaders and recruited new faculty who will be candidates for future advancement to research project leaders.

项目概要 阿肯色州综合代谢研究中心 (AIMRC) 是阿肯色大学的一期 COBRE 在费耶特维尔（04/01/2021-02/28/2026；PI：Kyle P. Quinn 博士）。 AIMRC 的科学目标是 通过涉及的研究调查细胞和组织代谢在疾病、发育和修复中的作用 先进成像、生物能量学和数据科学。 AIMRC 的长期目标是建立一个 可持续的跨学科研究中心，可以支持阿尔伯塔大学的生物医学研究并发展 校园代谢研究的新兴优势。生物能量学核心目前协助研究人员 测量细胞和分离组织水平的代谢通量。此补充将补充补充 通过实现啮齿动物的非破坏性和自动监测来同时监测新功能 体重、耗氧量、二氧化碳释放量以及运动过程中形成的水​​蒸气 通过购买 Promethion 代谢笼对这些实验动物进行研究。仪器将启用 研究人员评估浓缩重同位素 (13C/15N/2H) 的异常消耗/积累 在各种疾病条件下形成的代谢物。使用 Promethion 代谢核心获取的数据 有望提供有价值的信息，以了解各种潜在的分子机制 疾病。从 Promethion 代谢核心获得的信息可以与数据高度互补 使用其他可用的仪器设施（Seahorse 和 Oroboros 呼吸计）获得 在 AIMRC 生物能学核心。作为本补充剂的一部分购买的 Promethion 代谢笼 请求将为我们的校园和地区提供独特的资源。对研究工作将大有裨益 由现任研究项目负责人提出，并招募了新的教师，他们将成为未来的候选人 晋升为研究项目负责人。