Acquisition of multi-mode, high-resolution, high-sensitivity imaging platform

购置多模式、高分辨率、高灵敏度成像平台

• 批准号: 10629563
• 负责人: Annette H Erbse
• 金额: $ 15.28万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10629563
• 关键词: Aging; Basic Science; Binding; Biochemistry; Biological Assay; Biomedical Research; Color; Colorado; Communities; Complex; Core Facility; DNA; Data; Data Analyses; Data Collection; Densitometry; Detection; Drug Delivery Systems; Experimental Designs; Financial Hardship; Fluorescence; Funding; Future; Gel; Gene Expression Regulation; Health; Human; Image; Individual; Label; Life; Membrane; Membrane Biology; Methods; Noise; Pilot Projects; Proteins; Quality Control; RNA; Radioisotopes; Request for Applications; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Signal Transduction; Structure; Testing; Training; Translational Research; United States National Institutes of Health; Universities; antimicrobial; empowerment; experimental study; high resolution imaging; imager; imaging capabilities; imaging platform; improved; innovation; instrument; instrumentation; new therapeutic target; novel therapeutics; programs; small molecule; small molecule therapeutics; stoichiometry; success

Project Summary/Abstract This application requests funds to acquire a Typhoon 5 imaging platform, urgently needed to replace and upgrade the outdated and no longer factory-supported Typhoon 9500 in the Shared Instruments Pool (SIP) core facility in the Department of Biochemistry, University of Colorado Boulder. The Typhoon 5 is a high-sensitivity, high-resolution imaging platform for the imaging, characterization, and precise quantification of proteins, RNA, DNA, small molecules, and complexes. The multimode imager includes phospho imaging for radioisotope labeled samples, multi-color fluorescence detection (red, green, blue, and two near-infrared channels), calorimetric densitometry, and limited chemiluminescence. It can image in many different targets, including phospho storage screens, gels, membranes, TLC plates, multiwell plates, and microarrays. The extensive biomolecular imaging capabilities are essential for many biomedical research programs at CU Boulder. SIP provides a combination of cutting-edge instrumentation and well-maintained basic research instrumentation to the CU research community. The core facility widely and positively impacts many biomedical research programs at CU-Boulder, but this requires acquiring and maintaining advanced, state-of-the-art instrumentation. The current Typhoon 9500 in SIP has over the last five years served 182 individual users from 31 research groups in 5 departments and institutes at CU Boulder. A core group of 12 research labs - including 10 NIH-funded groups (a total of 15 ongoing NIH-funded projects) and two early-stage investigators from three departments - is currently relying heavily on the imaging capabilities of the Typhoon. The imager is used to collect publishable data (e.g., activity assays, binding stoichiometry, sequencing gels, and much more) and to quality control and optimize samples for downstream experiments. The new Typhoon 5 will serve existing biomedical research projects and will add new capabilities with two additional fluorescence channels in the NIR for increased sensitivity and improved signal-to-noise for challenging samples. The new Typhoon 5 in SIP will be available to all research groups at CU Boulder supported by the well- established management and financial structure that has served SIP and its research community well for the last 10 years. SIP staff will offer expertise and aid from experiment design to training, data collection, troubleshooting, and data analysis. This strong support structure empowers new and existing users to conduct pilot studies to test new experiments and research projects. It increases the success rate and minimizes the financial burden for research groups. This is essential for the future of biomedical research and will positively impact many new research programs at CU Boulder from innovative, basic research seeking to understand the fundamental mechanisms that govern human life and health (e.g. gene regulation, cell signaling, membrane biology, aging, and more) to translational research, for example, developing new drug targets, new therapeutic small molecules, antimicrobials, and drug delivery methods.

项目概要/摘要 本申请请求资金购买台风 5 号成像平台，急需更换和 升级共享仪器池 (SIP) 核心中过时且不再受工厂支持的 Typhoon 9500 科罗拉多大学博尔德分校生物化学系的设施。 Typhoon 5 是一款高灵敏度、 高分辨率成像平台，用于蛋白质、RNA、 DNA、小分子和复合物。多模式成像仪包括放射性同位素磷成像 标记样品，多色荧光检测（红、绿、蓝和两个近红外通道）， 量热密度测定法和有限化学发光法。它可以在许多不同的目标中成像，包括 磷酸储存屏、凝胶、膜、TLC 板、多孔板和微阵列。广泛的 生物分子成像能力对于科罗拉多大学博尔德分校的许多生物医学研究项目至关重要。 SIP 提供尖端仪器和维护良好的基础研究的结合 CU 研究界的仪器。核心设施对许多生物医学领域产生了广泛而积极的影响 科罗拉多大学博尔德分校的研究项目，但这需要获取和维护先进的、最先进的 仪器仪表。 SIP 现有的 Typhoon 9500 在过去五年中已为 182 名个人用户提供服务 科罗拉多大学博尔德分校 5 个系和研究所的 31 个研究小组。由 12 个研究实验室组成的核心小组 - 包括 10 个 NIH 资助的小组（总共 15 个正在进行的 NIH 资助项目）和来自三个研究小组的两名早期研究人员 部门 - 目前严重依赖台风的成像能力。成像仪用于 收集可发布的数据（例如，活性测定、结合化学计量、测序凝胶等）并 质量控制和优化下游实验的样品。新的台风 5 号将服务于现有的 生物医学研究项目，并将通过 NIR 中的两个额外荧光通道添加新功能 针对具有挑战性的样品提高灵敏度并改善信噪比。 苏州工业园区的新 Typhoon 5 将提供给 CU Boulder 的所有研究小组，并得到良好的支持。 建立的管理和财务结构为苏州工业园区及其研究界提供了良好的服务 10年。 SIP 工作人员将提供从实验设计到培训、数据收集、故障排除的专业知识和帮助， 和数据分析。这种强大的支持结构使新用户和现有用户能够进行试点研究 测试新的实验和研究项目。它提高了成功率并最大限度地减少了经济负担 对于研究小组。这对于生物医学研究的未来至关重要，并将对许多新的领域产生积极影响 科罗拉多大学博尔德分校的研究项目来自创新的基础研究，旨在了解基本原理 控制人类生命和健康的机制（例如基因调控、细胞信号传导、膜生物学、衰老、 等等）到转化研究，例如开发新的药物靶点、新的治疗小分子， 抗菌剂和药物输送方法。