MONOCLONAL ANTIBODY PRODUCTION

单克隆抗体生产

• 批准号: 8172729
• 负责人: KAREN S RICE
• 金额: $ 0.21万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172729
• 关键词: Animals; Antibodies; Biological Assay; Clinical Trials; Computer Retrieval of Information on Scientific Projects Database; Enzyme-Linked Immunosorbent Assay; Funding; Goals; Grant; Hemorrhage; Human; Immune Sera; Institution; Research; Research Personnel; Resources; Running; Serum; Source; Subcutaneous Injections; Techniques; Testing; United States National Institutes of Health; improved; monoclonal antibody production; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of this study was to produce antisera of an antibody testing in a clinical trial. To improve the assay to determine if anti-Abs are being produced, positive control material needed to be made. The antibody has been well tolerated, with only a few anti-Ab responses to date in human, not enough serum was produced to use as a positive control for all the assays run during the clinical trials. Previously used animal was used once again because of known antibodies and received weekly subcutaneous injections. When enough antibodies were present in the serum (as determined by ELISA techniques) a single large volume bleed was collected.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这项研究的目的是在临床试验中产生抗体测试的抗血清。 为了改善测定法以确定是否正在产生抗ABS，需要制作阳性对照材料。 抗体的耐受性很好，迄今为止在人类中只有少量抗AB反应，没有足够的血清来用作临床试验期间所有测定的阳性对照。由于已知的抗体，再次使用了先前使用的动物，并每周接受皮下注射。当血清中存在足够的抗体（按ELISA技术确定）时，收集了单个大体积出血。