Tissue Engineering Resource Center

组织工程资源中心

基本信息

批准号：
10213714
负责人：
DAVID L. KAPLAN
金额：
$ 32.78万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-16 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10213714
关键词：
3D Print Biocompatible Materials Biological Biological Process Biology Biomedical Engineering Biopolymers Biotin Carbodiimides Cells Chemistry Cicatrix Collaborations Collagen Complex Coupling Crystallization Deposition Devices Disease model Elastin Environment Enzymes Extracellular Matrix Fiber Fibrosis Free Radicals Freeze Drying Gel Genetic Engineering Goals Higher Order Chromatin Structure Hyaluronic Acid Immune response In Vitro Inflammation Light Mechanics Mediating Microfluidics Modification Natural regeneration Outcome Oxygen Polymers Polysaccharides Property Reaction Reactive Oxygen Species Regenerative Medicine Resources Series Signal Transduction Silk Site Source Stimulus Streptavidin Structural Protein Structure Study models System Temperature Therapeutic Time Tissue Engineering Tissue Model Tissues Variant Vascularization aqueous base biomaterial compatibility copolymer crosslink cytokine design dynamic system electric field experience functional restoration improved in vivo mechanical properties monomer nerve supply preservation public health relevance regeneration model response restoration scaffold self assembly tissue regeneration tissue support frame

项目摘要

SUMMARY TRD1 will focus on the design and implementation of adaptive-responsive biomaterials to meet the goals of the P41. These are biomaterials that can sense and actuate cells or respond to the local environment to drive the functional restoration of complex tissue structures, in vitro and in vivo. The hypothesis is that biomaterial systems with integrated features for activation/response can provide specific benefits for designing scaffolds for restoration of tissue structure and function. These new material systems will be integrated with the needs and goals of the other TRDs to optimize tissue outcomes at both the fundamental and translational levels. The ultimate goal is to develop biomaterial systems for functional restoration of complex tissue structures based on the response to changes in the local environment (intrinsic signaling) or via applied changes (extrinsic signaling). The plans are to develop materials that provide adaptive responses to changes in local biology and conditions (e.g., pH, temperature reactive oxygen, enzymes) or to external signals (e.g., light, electric fields) to effect a change to improve tissue function or regeneration goals. Control of mechanical properties, degradation and release of bioactive factors to respond to local changes are examples of dynamic response-control goals. The core biomaterials will be based on biopolymers (e.g., elastins, collagens, silks, hyaluronic acid, others) as bioengineered variants and composites. Three specific aims will be pursued. Aim 1: Biomaterials that dynamically change properties in response to local signals. These biopolymer systems will provide core functions for sensing-response using silk-elastin chemistry and composite designs with hyaluronic acid and collagen, for a broad range of utility for different cell and tissue needs. Aim 2: Biomaterials that change properties on demand, in response to external stimuli. The focus will be on light activation systems (via specific chemistries) or electric field-mediated changes (via incorporated conductive components). The goal is to control the material volume, mechanics, and delivery of bioactive factors in an on-demand mode, using external sources (light, electric field), to control cell and tissue outcomes. Aim 3: “Smart” scaffolds for tissue regeneration and modeling of disease. Scaffold designs based on the materials from Aims 1 and 2 will be utilized to generate functional devices and tissue models for studies in TRD1, 2 and 3, in vitro and in vivo.

概括 TRD1将专注于自适应响应生物材料的设计和实施，以满足 P41 的目标是这些生物材料可以感知和驱动细胞或对局部做出反应。驱动体外和体内复杂组织结构功能恢复的环境。假设具有激活/响应集成特征的生物材料系统可以提供这些新功能对于设计用于恢复组织结构和功能的支架具有特殊的好处。材料系统将与其他 TRD 的需求和目标相结合，以优化组织最终目标是开发生物材料。基于对变化的响应来恢复复杂组织结构的功能的系统本地环境（内在信号）或通过应用变化（外在信号）。开发能够对当地生物学和条件（例如 pH、pH、温度、活性氧、酶）或外部信号（例如光、电场）来影响改变以改善组织功能或再生目标控制机械性能、降解。和释放生物活性因子以响应局部变化是动态响应控制的例子核心生物材料将基于生物聚合物（例如弹性蛋白、胶原蛋白、丝、透明质酸）。酸、其他）作为生物工程变体和复合材料将实现三个具体目标：这些生物聚合物可根据局部信号动态改变特性。系统将利用丝弹性蛋白化学和复合材料提供传感响应的核心功能采用透明质酸和胶原蛋白设计，可满足不同细胞和组织的需求。目标 2：生物材料能够根据需要改变特性，以响应外部刺激。重点将放在光激活系统（通过特定化学物质）或电场介导的变化（通过合并的导电组件）的目标是控制材料体积、力学和使用外部来源（光、电场）以按需模式输送生物活性因子，以目标 3：用于组织再生和建模的“智能”支架。基于目标 1 和 2 的材料的支架设计将用于生成。用于 TRD1、2 和 3 体外和体内研究的功能装置和组织模型。