3D Intestinal Tissues

3D 肠道组织

• 批准号: 9312411
• 负责人: DAVID L. KAPLAN
• 金额: $ 30.55万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-07 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9312411
• 关键词: Address; Area; Biocompatible Materials; Biological; Biological Assay; Bioreactors; Cells; Clinic; Coculture Techniques; Colon; Communicable Diseases; Complex; Data; Development; Disease; Engineering; Enteral; Extracellular Matrix; Faculty; Frequencies; Goals; Human; Immune; Individual; Infection; Intestines; Laboratories; Mechanics; Modeling; Modification; Molds; Organoids; Outcome; Oxygen; Perfusion; Peristalsis; Pharmaceutical Preparations; Phase; Physiological; Population; Postdoctoral Fellow; Preparation; Quality Control; Reproducibility; Research; Research Project Grants; Robotics; Scaffolding Protein; Series; Silk; Small Intestines; Smooth Muscle; Source; Supplementation; Supporting Cell; System; Tissue Engineering; Tissue Model; Tissue Sample; Tissues; Training; Validation; Variant; Work; base; cell type; insight; pathogen; response; scaffold; scale up; systems research; tissue support frame

Core B – Summary Core B will utilize the improvements developed for the 3D intestinal systems to support the preparation and utility of the tissue models, including generating the scaffolds and seeded scaffolds/tissues for all projects. The plans are based on our extensive preliminary data where we have demonstrated structurally- and functionally- relevant 3D human intestinal tissues for the study of infectious diseases. The intestinal systems include enteroid primary cell sources, a 3D porous protein scaffolding system with a geometrically-engineered hollow lumen with adaptability to both large and small intestinal tissues. In Aim #1 the focus will be on the preparation of scaffolds and 3D intestinal systems for the three research projects. Here the goal is to provide intestinal tissues to support the three research projects (60 systems per week), obtain primary tissue samples from the clinic, establish early passage organoid material from both the small intestine and the colon, and train individuals in the various projects. In addition, a focus will be on documenting all materials for validation and quality control to assure reproducibility. In Aim #2 the focus will be on optimization of the 3D intestinal tissues. Here the goal is to continue to optimize the currently developed 3D human intestinal tissues generated from organoids for physiological relevance, and develop increasingly complex features to facilitate analysis of intestinal pathogens. These activities will include the incorporation of additional cell types, supplementation of the scaffolds with ECM components, and further development of a bioreactor system to control flow, peristalsis and oxygen levels. A team of faculty, post doctoral fellows and a technician will support the needs of Core B and work closely with the research teams on all phases of the plans.

核心 B – 总结 Core B 将利用为 3D 肠道系统开发的改进来支持准备和 组织模型的实用性，包括为所有项目生成支架和种子支架/组织。 计划基于我们广泛的初步数据，我们已经在结构和功能上证明了 用于传染病研究的相关 3D 人体肠道组织 肠道系统包括 肠样原代细胞来源，具有几何工程中空的 3D 多孔蛋白质支架系统 目标#1 的重点是制备对大肠和小肠组织都具有适应性的管腔。 这三个研究项目的支架和 3D 肠道系统的目标是提供肠道。 组织支持三个研究项目（每周 60 个系统），从 诊所，建立来自小肠和结肠的早期通道类器官材料，并进行训练 此外，重点将是记录所有材料以供验证和使用。 质量控制以确保可重复性 在目标 2 中，重点是 3D 肠道组织的优化。 这里的目标是继续优化目前开发的 3D 人体肠道组织 类器官具有生理相关性，并越来越多地开发复杂的特征以促进分析 这些活动将包括纳入其他细胞类型、补充 具有 ECM 组件的支架，以及进一步开发生物反应器系统来控制流动、蠕动 由教师、博士后研究员和技术人员组成的团队将支持核心 B 的需求。 并与研究团队在计划的各个阶段密切合作。