Core C

核心C

• 批准号: 7407113
• 负责人: John D Groopman
• 金额: $ 10.58万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7407113
• 关键词: Address; Area; Arts; Bioconductor; Bioinformatics; Biostatistics Core; Core Facility; Data Analyses; Environmental Exposure; Epigenetic Process; Future; Gene Chips; Gene Expression; Genes; Goals; Grant; Human Genome; Instruction; Methods; Molecular Biology; Molecular Genetics; Proteomics; Rattus; Research Infrastructure; Research Personnel; Resources; Technology; Training; functional genomics; genetic analysis; instrumentation; medical schools; member; mouse genome; tool

B3. Molecular Genetics and Epigenetics The Molecular Genetics and Epigenetics component of the IHSFC, formerly called Facility Core C: Microarray and Molecular Biology, has been transformed to address the expressed current and future needs of Center members for instrumentation and expertise support in the areas of gene expression, epigenetics and functional genomics. The goal of this activity is to provide to Center members access to cutting edge technologies in the analysis of gene expression and modulation through access to instrumentation, instruction, assistance in methods and training in the analysis of data. We have added the epigenetics analysis to this iteration of the grant, replacing resources that had been previously directed towards proteomics. This decision was made because the Proteomics Core at Johns Hopkins School of Medicine is (http://www.hopkinsmedicine.org/msf/) now widely available for Center members and we choose not to duplicate these efforts. The objectives of this component of the IHSFC are to: ¿ provide state-of-the-art analysis of gene expression and analysis using the Affymetrix platform for mouse (genome 430A 2.0 array), rat (expression 230 2.0) and human (genome U133 Plus 2.0 array) gene chips that comprise the global gene profiling for these respective species; ¿ provide infrastructure so that an investigator can understand the global gene expression changes modified by environmental exposures that are manifested through epigenetic changes; ¿ integrate with the Bioinformatics/Biostatistics core for using Bioconductor and other tools for the analysis of genetic and epigenetic changes.

B3。分子遗传学和表观遗传学 IHSFC的分子遗传学和表观遗传学成分，以前称为设施核心C： 微阵列和分子生物学已转化，以满足表达的当前和未来需求 基因表达，表观遗传学和专业知识支持的中心成员 功能基因组学。这项活动的目的是为中心成员访问前沿 通过访问仪器，指令，分析基因表达和调节的技术 在数据分析中的方法和培训方面的帮助。我们为此添加了表观遗传学分析 赠款的迭代，取代以前针对蛋白质组学的资源。这个决定 之所以成为约翰·霍普金斯医学院的蛋白质组学核心是 （http://www.hopkinsmedicine.org/msf/）现在广泛用于中心成员，我们选择不 复制这些努力。 IHSFC的该组成部分的目标是： »使用小鼠的affymetrix平台提供基因表达和分析的最新分析 （基因组430a 2.0阵列），大鼠（表达230 2.0）和人（基因组U133加2.0阵列）基因芯片 完成这些相对物种的全球基因分析； �提供基础架构，以便研究人员可以理解全局基因表达变化的变化 通过通过表观遗传变化体现的环境暴露； »与生物信息学/生物统计学的核心集成在一起，用于使用生物导体和其他工具分析 遗传和表观遗传变化。