INTERDEPENDENCE OF YEAST RIBOSOME ASSEMBLY FACTORS

酵母核糖体组装因素的相互依赖性

• 批准号: 7602107
• 负责人: JOHN L. WOOLFORD
• 金额: $ 0.62万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7602107
• 关键词: Address; Binding; Binding Sites; Biogenesis; C-terminal; Chromosome Segregation; Computer Retrieval of Information on Scientific Projects Database; Dominant-Negative Mutation; Funding; Genome; Grant; Institution; Length; Mitotic Chromosome; Nucleolar Proteins; Pathway interactions; Process; Protein Overexpression; Proteins; Research; Research Personnel; Resources; Ribosomes; Source; United States National Institutes of Health; Yeasts; cell growth; rRNA Precursor; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ytm1, Nop7, and Erb1 are essential, conserved nucleolar proteins necessary for assembly of 60S ribosomal subunits. Each of these three proteins is dependent on the other for their assembly into and stable association with preribosomes. In their absence, processing of 27SA3 pre-rRNA to 27SB pre-rRNA is blocked. Interaction between the central domain of Nop7 with the conserved amino-terminal middle of Erb1 is required for their recruitment into pre-rRNPs. The WD40 motif of Ytm1 then binds to Erb1 amino-terminal to the Nop7 binding site. Consistent with these domains of interaction, expression of truncated Ytm1 or Erb1 constructs containing only these interaction domains enables the proteins to associate with each other, assemble into preribosomes, and cause a dominant negative effect on ribosome biogenesis. What are the functions of the conserved amino-terminal half of Ytm1 or the conserved WD40 motif in the C-terminal half of Erb1, which are missing in these dominant-negative truncations? Erb1 also may function in cell growth and proliferation; overexpression of Erb1 interferes with mitotic chromosome segregation. How does Erb1 participate in this pathway? To address these questions, we are carrying out genome-wide two-hybrid screens using the amino-terminal half of Ytm1, the C-terminal half of Erb1 and full-length Erb1. Interactions of these portions of Erb1 and Ytm1 with other molecules in assembling ribosomes may be critical to establish proper dynamics of ribosome biogenesis.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 YTM1，NOP7和ERB1是必不可少的，保守的核仁蛋白，用于组装60S核糖体亚基。 这三种蛋白质中的每一个都取决于另一个蛋白质，以使其组装成与前体的稳定关联。在不存在的情况下，将27SA3前RRNA处理至27SB前RRNA被阻断。 NOP7的中央结构域与ERB1的保守氨基末端中间之间的相互作用是将其募集到PreRNP中所必需的。然后，YTM1的WD40基序与NOP7结合位点与ERB1氨基末端结合。 与这些相互作用的域一致，仅包含这些相互作用结构域的截短的YTM1或ERB1构建体的表达使蛋白质可以彼此相连，组装成蛋白质体，并对核糖体生物发生产生显着的负面影响。在ERB1的C末端一半中，保守的YTM1的保守氨基末端一半或保守的WD40基序的功能是什么？ ERB1也可能在细胞生长和增殖中发挥作用。 ERB1的过表达会干扰有丝分裂染色体分离。 ERB1如何参与这一途径？ 为了解决这些问题，我们正在使用YTM1的氨基末端半末端，ERB1的C末端和全长ERB1进行全基因组的两个杂交筛选。 ERB1和YTM1与组装核糖体中其他分子的相互作用对于建立适当的核糖体生物发生动力学至关重要。