RIBOSOME ASSEMBLY

核糖体组装

• 批准号: 7420754
• 负责人: JOHN L. WOOLFORD
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420754
• 关键词: proteins; ribosomal proteins; ribosomes

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In eukaryotes, 80 ribosomal proteins and more than 170 ribosome assembly factors must interact with each other and with rRNA in a coordinated fashion to produce functional ribosomal subunits. However, in most cases, which of these proteins function together and the substrates upon which they act have not been identified. It is now important to establish interactions between these ribosomal proteins or assembly factors and other proteins. We have identified two subcomplexes present in assembling ribosomes--the Nop7/Ytm1/Erb1 subcomplex and the Rpf2/Rrs1/rpL5/rpL11/5SrRNA subcomplex. Interestingly, Ytm1,Erb1, and Nop7 also have been implicated in other cellular processes, including DNA replication and microtubule function. To better understand the roles of these proteins in ribosome biogenesis and in other pathways, we are using each protein or domains within each protein, as baits in a systematic two-hybrid screen to identify interacting proteins.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。在真核生物中，80个核糖体蛋白和170多个核糖体组装因子必须相互相互作用，并以协调的方式与rRNA相互作用，以产生功能性核糖体亚基。但是，在大多数情况下，这些蛋白质中的哪一种以及它们作用的底物尚未鉴定出来。现在，重要的是在这些核糖体蛋白或组装因子和其他蛋白质之间建立相互作用。我们已经确定了组装核糖体中存在的两个子复合物：NOP7/YTM1/ERB1子复合物和RPF2/RRS1/RPL5/RPL5/RPL11/5SRRRNA子复合物。有趣的是，YTM1，ERB1和NOP7也与其他细胞过程有关，包括DNA复制和微管功能。为了更好地理解这些蛋白质在核糖体生物发生和其他途径中的作用，我们正在使用每种蛋白质中的每种蛋白质或结构域，作为系统的两个杂交筛选中的诱饵来识别相互作用的蛋白质。