LIGANDS OF MOONLIGHTING RIBOSOME ASSEMBLY FACTORS

兼职核糖体组装因子的配体

• 批准号: 7420671
• 负责人: JOHN L. WOOLFORD
• 金额: $ 0.5万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420671
• 关键词: library; proteins; ribosomes

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have identified several essential, conserved proteins that are present in 66S pre-ribosomes and necessary for ribosome assembly. In order to identify neighbors of these proteins in assembling ribosomes or to explore for moonlighting partners, we have begun screening the P. James library for partners of each protein. We would also like to collaborate with the YRC to screen your ordered two-hybrid library, using each of these proteins as bait.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。我们已经确定了几种基本保守的蛋白质，这些蛋白质存在于66S前圆柱体中，并且是核糖体组装所必需的。为了鉴定这些蛋白质的邻居在组装核糖体或探索月光伴侣时，我们已经开始为每种蛋白质的伴侣筛选P. James库。我们还想与YRC合作，以使用这些蛋白质作为诱饵进行筛选您的订购的两个杂交库。